GEN1 (NM_182625) Human Tagged ORF Clone

CAT#: RC221451

GEN1 (Myc-DDK-tagged)-Human Gen homolog 1, endonuclease (Drosophila) (GEN1), transcript variant 1

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USD 629.00


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Size
    • 10 ug

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Specifications

Product Data
Product Name GEN1 (NM_182625) Human Tagged ORF Clone
Symbol GEN1
Synonyms Gen; Gen
Vector pCMV6-Entry
Sequence Data
ORF Nucleotide Sequence
>RC221451 representing NM_182625
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGAGTGAATGACTTGTGGCAAATTTTGGAGCCTGTTAAGCAACACATCCCCTTGCGTAATCTTGGTG
GGAAAACCATTGCAGTTGATCTGAGTCTCTGGGTGTGTGAGGCACAGACAGTCAAAAAAATGATGGGCAG
CGTCATGAAGCCCCACCTCAGGAACTTATTTTTTCGTATCTCATATTTAACACAAATGGATGTAAAACTG
GTATTTGTTATGGAAGGGGAACCACCAAAGCTGAAAGCTGATGTCATAAGCAAGAGGAATCAGACTCGGT
ATGGGTCTTCTGGAAAATCGTGGTCTCAGAAAACAGGGAGATCACATTTTAAATCAGTCTTAAGAGAGTG
CCTCCATATGCTCGAATGCTTAGGAATCCCCTGGGTTCAGGCTGCTGGGGAAGCTGAAGCCATGTGTGCT
TATCTCAATGCTGGTGGTCATGTCGATGGCTGCCTCACCAATGATGGAGATACTTTCCTTTATGGGGCCC
AGACTGTTTACAGGAATTTCACTATGAATACAAAGGACCCACATGTTGACTGTTACACAATGTCATCTAT
CAAGAGTAAACTAGGTTTGGATAGAGATGCTCTGGTTGGATTAGCAATACTTCTTGGCTGTGATTATCTC
CCAAAGGGAGTCCCTGGAGTTGGAAAAGAGCAAGCATTAAAACTTATACAGATTTTGAAAGGGCAAAGTT
TACTTCAGAGGTTTAATCGGTGGAATGAAACATCTTGTAACTCTAGTCCACAACTGCTAGTCACTAAAAA
ACTGGCTCATTGTTCCGTATGTTCCCATCCAGGTTCACCTAAGGATCATGAACGTAATGGATGCAGATTA
TGTAAAAGTGATAAATATTGTGAGCCACATGACTATGAATACTGCTGTCCTTGTGAGTGGCACCGTACAG
AACATGATAGGCAACTCAATGAAGTAGAGAACAATATTAAGAAGAAAGCTTGCTGTTGTGAGGGATTCCC
ATTCCATGAGGTTATTCAAGAATTCCTTTTAAACAAGGATAAATTGGTGAAGGTTATCAGGTACCAAAGA
CCTGATTTGTTATTGTTTCAGAGATTTACTCTTGAAAAAATGGAGTGGCCCAATCACTATGCATGTGAGA
AATTGCTGGTACTTTTGACCCATTATGACATGATAGAAAGAAAGCTTGGTAGCAGAAACTCTAATCAACT
ACAGCCAATTCGAATTGTTAAGACTCGAATCAGAAATGGAGTTCATTGTTTTGAAATAGAATGGGAAAAG
CCTGAACATTATGCTATGGAAGATAAACAACATGGAGAATTTGCTTTATTAACAATTGAGGAAGAATCAT
TGTTTGAAGCAGCATATCCTGAGATCGTTGCTGTTTACCAAAAACAAAAGTTAGAAATTAAAGGGAAGAA
ACAAAAACGTATTAAGCCTAAAGAAAACAATTTGCCAGAACCAGATGAAGTAATGAGCTTTCAGTCACAC
ATGACTTTAAAACCCACATGTGAAATCTTTCATAAGCAGAATTCCAAGTTAAATTCGGGGATTTCCCCTG
ATCCTACATTACCACAGGAATCTATTTCTGCCTCATTGAATAGCTTGCTTTTACCTAAAAATACTCCATG
TTTGAATGCACAAGAACAGTTCATGTCTTCTCTAAGACCTTTGGCTATACAGCAAATTAAAGCTGTCAGT
AAGTCTCTAATTTCAGAATCTAGTCAACCCAATACCTCATCTCATAATATATCCGTGATTGCTGATCTAC
ACTTGAGCACTATTGACTGGGAAGGTACTTCTTTTAGTAATTCTCCAGCTATTCAAAGGAATACTTTTTC
TCATGATTTAAAATCAGAAGTTGAATCAGAGCTATCAGCCATCCCTGATGGCTTTGAAAATATCCCAGAA
CAACTGTCCTGTGAATCAGAAAGGTACACTGCAAACATAAAGAAAGTGTTGGATGAGGATTCTGATGGGA
TTAGTCCTGAAGAGCATCTACTTTCTGGCATTACTGATTTATGTCTTCAGGATTTGCCTTTAAAGGAACG
AATATTTACAAAATTATCATATCCTCAGGATAATCTACAACCAGATGTCAACCTGAAAACTTTGTCCATA
CTTAGTGTAAAAGAATCTTGTATTGCTAACAGTGGTTCTGATTGTACATCACATCTTTCAAAGGATCTTC
CAGGAATTCCCTTGCAAAATGAATCCAGAGACTCTAAAATTCTAAAAGGAGACCAGCTGCTTCAAGAAGA
CTATAAAGTCAATACTTCTGTCCCTTATTCTGTCAGTAACACAGTGGTAAAGACCTGCAATGTTAGACCA
CCAAATACTGCTTTAGATCATAGTAGAAAAGTTGATATGCAAACCACTCGGAAAATTTTAATGAAGAAGA
GTGTTTGCCTTGACAGACATTCCTCTGATGAACAAAGTGCCCCAGTGTTTGGGAAAGCTAAGTACACAAC
TCAAAGAATGAAGCACAGTTCTCAAAAGCATAATTCATCCCATTTCAAAGAAAGTGGCCATAACAAGTTG
AGTAGCCCTAAGATACATATTAAAGAAACTGAACAGTGTGTCAGATCTTATGAAACAGCTGAAAATGAAG
AAAGCTGTTTCCCAGATTCAACAAAAAGTTCTCTGAGTTCTCTACAATGTCATAAGAAAGAAAACAACTC
TGGTACTTGTTTGGATAGCCCTCTTCCTTTACGCCAGAGATTAAAACTAAGATTCCAAAGCACT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC221451 representing NM_182625
Red=Cloning site Green=Tags(s)

MGVNDLWQILEPVKQHIPLRNLGGKTIAVDLSLWVCEAQTVKKMMGSVMKPHLRNLFFRISYLTQMDVKL
VFVMEGEPPKLKADVISKRNQTRYGSSGKSWSQKTGRSHFKSVLRECLHMLECLGIPWVQAAGEAEAMCA
YLNAGGHVDGCLTNDGDTFLYGAQTVYRNFTMNTKDPHVDCYTMSSIKSKLGLDRDALVGLAILLGCDYL
PKGVPGVGKEQALKLIQILKGQSLLQRFNRWNETSCNSSPQLLVTKKLAHCSVCSHPGSPKDHERNGCRL
CKSDKYCEPHDYEYCCPCEWHRTEHDRQLNEVENNIKKKACCCEGFPFHEVIQEFLLNKDKLVKVIRYQR
PDLLLFQRFTLEKMEWPNHYACEKLLVLLTHYDMIERKLGSRNSNQLQPIRIVKTRIRNGVHCFEIEWEK
PEHYAMEDKQHGEFALLTIEEESLFEAAYPEIVAVYQKQKLEIKGKKQKRIKPKENNLPEPDEVMSFQSH
MTLKPTCEIFHKQNSKLNSGISPDPTLPQESISASLNSLLLPKNTPCLNAQEQFMSSLRPLAIQQIKAVS
KSLISESSQPNTSSHNISVIADLHLSTIDWEGTSFSNSPAIQRNTFSHDLKSEVESELSAIPDGFENIPE
QLSCESERYTANIKKVLDEDSDGISPEEHLLSGITDLCLQDLPLKERIFTKLSYPQDNLQPDVNLKTLSI
LSVKESCIANSGSDCTSHLSKDLPGIPLQNESRDSKILKGDQLLQEDYKVNTSVPYSVSNTVVKTCNVRP
PNTALDHSRKVDMQTTRKILMKKSVCLDRHSSDEQSAPVFGKAKYTTQRMKHSSQKHNSSHFKESGHNKL
SSPKIHIKETEQCVRSYETAENEESCFPDSTKSSLSSLQCHKKENNSGTCLDSPLPLRQRLKLRFQST

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
Tag Myc-DDK
ACCN NM_182625
ORF Size 2724 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reference Data
RefSeq NM_182625.2, NM_182625.3, NM_182625.4, NP_872431
RefSeq Size 3024
RefSeq ORF 2727
Locus ID 348654
Cytogenetics 2p24.2
MW 102.7 kDa
Gene Summary This gene encodes a member of the Rad2/xeroderma pigmentosum group G nuclease family, whose members are characterized by N-terminal and internal xeroderma pigmentosum group G nuclease domains followed by helix-hairpin-helix domains and disordered C-terminal domains. The protein encoded by this gene is involved in resolution of Holliday junctions, which are intermediate four-way structures that covalently link DNA during homologous recombination and double-strand break repair. The protein resolves Holliday junctions by creating dual incisions across the junction to produce nicked duplex products that can be ligated. In addition, this protein has been found to localize to centrosomes where it has been implicated in regulation of centrosome integrity. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2016].
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Citations