ACCSL (NM_001031854) Human Tagged ORF Clone

CAT#: RC221449

ACCSL (Myc-DDK-tagged)-Human 1-aminocyclopropane-1-carboxylate synthase homolog (Arabidopsis)(non-functional)-like (ACCSL)

Product Images

Circular map for RC221449

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  Western blot validation of overexpression lysate (Cat# LY422209) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC221449 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified ACCSL protein (Cat# TP321449). The protein was produced from HEK293T cells transfected with ACCSL cDNA clone (Cat# RC221449) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ACCSL
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC221449 representing NM_001031854
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTCATCGGTCAGACACCCTTCCTGTGCCCTCTGGTCAGAGGAGAGGCCGGGTCCCCAGAGACCACA
  GCATCTATACCCAGCTGTTGGAGATAACGCTGCACTTGCAGCAGGCCATGACGGAGCACTTCGTGCAGCT
  GACGAGCAGACAGGGCCTGTCGCTGGAGGAAAGGAGGCACACTGAGGCCATCTGTGAGCATGAAGCCCTT
  CTGAGTCGCTTAATATGCCGGATGATCAACCTCCTACAGTCTGGGGCCGCGAGTGGCCTGGAGCTCCAAG
  TGCCTCTTCCTTCTGAGGACTCTAGGGGTGATGTCAGATATGGGCAGAGGGCCCAACTCTCTGGGCAGCC
  TGATCCAGTTCCCCAACTGAGTGATTGTGAAGCTGCCTTTGTCAACCGCGACCTATCCATCCGTGGGATT
  GACATCTCTGTCTTTTATCAGTCGAGCTTCCAGGACTACAATGCCTACCAAAAAGATAAATATCATAAGG
  ACAAGAACACCTTGGGTTTCATTAACCTTGGCACCAGTGAGAACAAGCTCTGCATGGATCTGATGACTGA
  AAGATTGCAAGAAAGTGACATGAACTGCATTGAGGACACCTTGCTTCAGTACCCTGATTGGAGAGGGCAG
  CCATTCCTGCGGGAAGAAGTGGCCCGGTTCCTGACCTACTACTGCAGGGCACCTACCCGACTTGACCCAG
  AAAATGTGGTGGTTCTAAATGGCTGCTGCTCTGTCTTCTGTGCCCTGGCCATGGTTCTGTGTGATCCAGG
  CGAGGCCTTCCTGGTCCCTGCTCCCTTCTATGGTGGCTTTGCCTTTAGCTCCCGCCTGTATGCAAAGGTT
  GAGTTGATTCCTGTCCACCTGGAGAGTGAGGTCACTGTTACAAACACCCATCCTTTCCAGCTCACTGTGG
  ACAAGTTAGAGGAAGCCCTGCTTGAAGCTAGGCTTGAGGGGAAAAAGGTCCGAGGCCTTGTGCTAATCAA
  CCCTCAGAATCCTCTGGGTGACATCTACTCCCCAGACTCACTGATGAAATACCTGGAATTTGCCAAGAGG
  TATAACCTACATGTGATCATAGATGAGATTTACATGCTGTCTGTGTTTGATGAATCCATCACATTCCACA
  GCATTCTGAGCATGAAAAGTTTGCCTGATAGCAACAGGACCCATGTGATCTGGGGTACCAGTAAGGATTT
  TGGCATCTCTGGCTTCCGCTTTGGTGCTCTGTATACCCACAACAAGGAGGTGGCCTCTGCTGTGAGTGCC
  TTTGGCTACCTCCACAGTATTTCTGGCATCACCCAGCACAAGCTGTGTCAACTGCTCCAGAACACAGAAT
  GGATTGACAAAGTATACCTACCCACCAATTGCTACCGGCTCCGGGAAGCTCACAAGTACATCACTGCTGA
  GCTGAAGGCATTGGAGATCCCTTTTCACAACCGCAGCTCTGGCCTCTATGTCTGGATCAACTTGAAAAAG
  TACCTGGATCCCTGTACATTTGAAGAAGAACGGCTCCTCTATTGCCGCTTCCTGGACAACAAGCTATTGT
  TATCCCGTGGCAAAACCTACATGTGTAAGGAGCCAGGCTGGTTCTGCCTCATCTTTGCAGATGAGCTCCC
  CCGGCTAAAATTGGCTATGCGTCGGTTCTGTGATGTGCTGCAGGAGCAGAAGGAGGCTTTGATAGTGAAG
  CAGTTGGAGGATGCAATGAGGGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC221449 representing NM_001031854
  Red=Cloning site Green=Tags(s)
  
  MSHRSDTLPVPSGQRRGRVPRDHSIYTQLLEITLHLQQAMTEHFVQLTSRQGLSLEERRHTEAICEHEAL
  LSRLICRMINLLQSGAASGLELQVPLPSEDSRGDVRYGQRAQLSGQPDPVPQLSDCEAAFVNRDLSIRGI
  DISVFYQSSFQDYNAYQKDKYHKDKNTLGFINLGTSENKLCMDLMTERLQESDMNCIEDTLLQYPDWRGQ
  PFLREEVARFLTYYCRAPTRLDPENVVVLNGCCSVFCALAMVLCDPGEAFLVPAPFYGGFAFSSRLYAKV
  ELIPVHLESEVTVTNTHPFQLTVDKLEEALLEARLEGKKVRGLVLINPQNPLGDIYSPDSLMKYLEFAKR
  YNLHVIIDEIYMLSVFDESITFHSILSMKSLPDSNRTHVIWGTSKDFGISGFRFGALYTHNKEVASAVSA
  FGYLHSISGITQHKLCQLLQNTEWIDKVYLPTNCYRLREAHKYITAELKALEIPFHNRSSGLYVWINLKK
  YLDPCTFEEERLLYCRFLDNKLLLSRGKTYMCKEPGWFCLIFADELPRLKLAMRRFCDVLQEQKEALIVK
  QLEDAMRE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001031854
• ORF Size: 1704 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001031854.2, NP_001027025.2
• RefSeq Size: 1820 bp
• RefSeq ORF: 1707 bp
• Locus ID: 390110
• UniProt ID: Q4AC99
• Cytogenetics: 11p11.2
• MW: 65.1 kDa