SENP7 (NM_001077203) Human Tagged ORF Clone

CAT#: RC221415

SENP7 (Myc-DDK-tagged)-Human SUMO1/sentrin specific peptidase 7 (SENP7), transcript variant 2

Product Images

Circular map for RC221415

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SENP7
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC221415 representing NM_001077203
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACAAGAGAAAGCTCGGGCGACGGCCATCTTCATCCGAAATCATCACAGAAGGAAAAAGGAAAAAGT
  CATCTTCTGATTTATCGGAGATAAGAAAGATGTTAAATGCAAAACCAGAGGATGTCCATGTTCAATCACC
  ACTGTCCAAATTCAGAAGCTCAGAACGCTGGACTCTCCCTTTGCAGTGGGAAAGAAGCCTAAGGAATAAA
  GTCATCTCTCTAGACCATAAAAATAAAAAACATATCCGAGGGTGTCCTGTTACTTCCAAGTCATCACCAG
  AAAGGCAACTCAAAGTTATGTTGACGAATGTCCTATGGACGGATTTAGGACGAAAATTCAGAAAGACCCT
  ACCTAGAAACGATGCTAATTTATGTGATGCCAACAAGGTGCAATCAGACTCATTGCCTTCGACATCTGTT
  GACAGCCTAGAGACATGTCAAAAATTAGAACCTCTTCGCCAAAGCCTTAATTTATCTGAAAGGGGCTCAC
  AACGAAGTAAGACAGTAGATGACAATTCTGCAAAGCAGACTGCGCACAATAAAGAAAAACGAAGAAAGGA
  TGATGGCATTTCTCTTTTAATATCTGATACTCAGCCTGAAGACCTTAACAGTGGAAGTAGAGGTTGTGAT
  CATCTCGAACAGGAAAGCAGAAACAAGGATGTTAAATATTCTGATTCAAAAGTGGAACTCACTCTGATTT
  CCAGGAAGACAAAGAGAAGGCTTAGAAATAATTTACCTGATTCTCAATATTGTACTTCTTTGGATAAGTC
  AACAGAACAGACAAAAAAACAAGAAGATGACTCAACAATATCCACTGAGTTTGAAAAGCCAAGTGAAAAC
  TATCATCAGGATCCAAAACTGCCTGAAGAAATTACAACTAAACCTACAAAAAGTGATTTTACTAAACTAT
  CCTCACTTAACAGTCAGGAGTTGACTTTGAGTAATGCCACCAAAAGTGCCTCTGCCGGTTCAACCACTGA
  AACCGTTGAGAACTCTAATTCCATTGATATTGTGGGGATTTCTTCCCTGGTTGAGAAGGATGAGAATGAG
  TTGAATACCATAGAAAAGCCTATTCTAAGAGGACATAATGAAGGGAACCAATCACTGATCTCAGCTGAAC
  CAATTGTTGTTTCCAGTGATGAAGAAGGACCTGTTGAACATAAAAGTTCAGAAATTCTTAAGTTACAATC
  TAAGCAAGACCGTGAGACAACTAATGAAAATGAGAGTACTTCTGAATCAGCATTGTTAGAACTACCATTG
  ATTACATGTGAATCTGTACAGATGTCATCTGAATTATGCCCATATAATCCTGTCATGGAGAACATTTCCA
  GTATTATGCCTAGTAATGAGATGGATCTACAACTGGATTTTATATTTACTTCTGTTTATATTGGTAAAAT
  AAAAGGAGCTTCTAAAGGTTGTGTTACAATCACAAAAAAATATATTAAGATCCCATTTCAAGTGTCCCTG
  AATGAGATTTCATTGCTAGTGGATACCACACATTTAAAGCGGTTTGGGTTATGGAAAAGTAAGGATGATA
  ATCACAGTAAAAGGAGTCATGCTATTCTTTTCTTCTGGGTCTCTTCAGATTATCTTCAAGAGATTCAGAC
  CCAATTAGAACACTCTGTATTAAGCCAGCAATCAAAATCTAGTGAATTCATTTTCCTTGAACTACACAAT
  CCTGTTTCACAAAGAGAAGAATTGAAGCTGAAAGATATTATGACGGAAATAAGTATAATCAGTGGAGAAT
  TAGAGCTTTCTTACCCGTTGTCTTGGGTTCAGGCATTTCCTTTGTTTCAGAACCTCTCTTCAAAAGAAAG
  TTCTTTTATTCATTATTACTGTGTTTCAACTTGTTCTTTCCCTGCTGGTGTTGCTGTTGCTGAAGAAATG
  AAGCTGAAATCAGTATCTCAGCCCTCAAACACAGATGCGGCCAAGCCTACTTACACCTTCCTGCAGAAGC
  AAAGTAGCGGTTGCTACTCCCTTTCTATTACATCTAATCCAGATGAAGAATGGCGAGAAGTCAGGCACAC
  TGGACTTGTTCAGAAGTTGATTGTATATCCTCCACCACCTACTAAGGGGGGATTAGGAGTAACTAATGAA
  GATCTGGAGTGTTTAGAAGAAGGAGAGTTTCTTAATGATGTAATCATTGATTTTTACCTTAAGTATCTTA
  TATTGGAGAAGGCATCAGATGAACTTGTTGAACGAAGTCACATTTTTAGTAGCTTTTTCTATAAATGCTT
  GACAAGAAAGGAAAATAATTTAACAGAAGATAATCCAAATCTTTCAATGGCACAGAGAAGACATAAAAGA
  GTAAGAACATGGACTCGTCACATAAACATTTTTAATAAAGATTACATCTTTGTACCTGTAAATGAGTCGT
  CTCACTGGTATCTCGCAGTCATTTGTTTTCCATGGTTAGAAGAAGCTGTGTATGAAGATTTTCCACAAAC
  TGTATCCCAGCAGTCCCAGGCTCAGCAGTCCCAAAATGACAACAAAACAATAGATAATGATCTACGTACT
  ACTTCGACACTGTCTTTGAGTGCAGAGGATTCCCAAAGTACCGAGTCGAATATGTCAGTACCAAAGAAAA
  TGTGTAAAAGGCCATGTATTCTTATACTAGACTCCTTGAAAGCTGCTTCTGTACAAAACACAGTTCAGAA
  TTTACGAGAGTATTTAGAGGTAGAGTGGGAAGTTAAACTAAAAACTCATCGTCAATTCAGCAAAACAAAC
  ATGGTGGATCTATGCCCTAAAGTTCCTAAACAGGACAATAGCAGTGATTGTGGAGTATATTTATTGCAGT
  ATGTGGAAAGCTTCTTCAAGGATCCTATTGTTAACTTTGAACTTCCAATTCATTTGGAGAAGTGGTTTCC
  TCGTCATGTAATAAAGACCAAACGGGAAGATATTCGAGAGCTCATCTTGAAACTTCATTTACAGCAACAG
  AAGGGCAGCAGTAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC221415 representing NM_001077203
  Red=Cloning site Green=Tags(s)
  
  MDKRKLGRRPSSSEIITEGKRKKSSSDLSEIRKMLNAKPEDVHVQSPLSKFRSSERWTLPLQWERSLRNK
  VISLDHKNKKHIRGCPVTSKSSPERQLKVMLTNVLWTDLGRKFRKTLPRNDANLCDANKVQSDSLPSTSV
  DSLETCQKLEPLRQSLNLSERGSQRSKTVDDNSAKQTAHNKEKRRKDDGISLLISDTQPEDLNSGSRGCD
  HLEQESRNKDVKYSDSKVELTLISRKTKRRLRNNLPDSQYCTSLDKSTEQTKKQEDDSTISTEFEKPSEN
  YHQDPKLPEEITTKPTKSDFTKLSSLNSQELTLSNATKSASAGSTTETVENSNSIDIVGISSLVEKDENE
  LNTIEKPILRGHNEGNQSLISAEPIVVSSDEEGPVEHKSSEILKLQSKQDRETTNENESTSESALLELPL
  ITCESVQMSSELCPYNPVMENISSIMPSNEMDLQLDFIFTSVYIGKIKGASKGCVTITKKYIKIPFQVSL
  NEISLLVDTTHLKRFGLWKSKDDNHSKRSHAILFFWVSSDYLQEIQTQLEHSVLSQQSKSSEFIFLELHN
  PVSQREELKLKDIMTEISIISGELELSYPLSWVQAFPLFQNLSSKESSFIHYYCVSTCSFPAGVAVAEEM
  KLKSVSQPSNTDAAKPTYTFLQKQSSGCYSLSITSNPDEEWREVRHTGLVQKLIVYPPPPTKGGLGVTNE
  DLECLEEGEFLNDVIIDFYLKYLILEKASDELVERSHIFSSFFYKCLTRKENNLTEDNPNLSMAQRRHKR
  VRTWTRHINIFNKDYIFVPVNESSHWYLAVICFPWLEEAVYEDFPQTVSQQSQAQQSQNDNKTIDNDLRT
  TSTLSLSAEDSQSTESNMSVPKKMCKRPCILILDSLKAASVQNTVQNLREYLEVEWEVKLKTHRQFSKTN
  MVDLCPKVPKQDNSSDCGVYLLQYVESFFKDPIVNFELPIHLEKWFPRHVIKTKREDIRELILKLHLQQQ
  KGSSS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001077203
• ORF Size: 2955 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001077203.3
• RefSeq Size: 4739 bp
• RefSeq ORF: 2958 bp
• Locus ID: 57337
• UniProt ID: Q9BQF6
• Cytogenetics: 3q12.3
• Protein Families: Druggable Genome, Protease
• MW: 112.3 kDa
• Gene Summary: The reversible posttranslational modification of proteins by the addition of small ubiquitin-like SUMO proteins (see SUMO1; MIM 601912) is required for many cellular processes. SUMO-specific proteases, such as SENP7, process SUMO precursors to generate a C-terminal diglycine motif required for the conjugation reaction. They also display isopeptidase activity for deconjugation of SUMO-conjugated substrates (Lima and Reverter, 2008 [PubMed 18799455]).[supplied by OMIM, Jun 2009]