A1BG (NM_130786) Human Tagged ORF Clone

CAT#: RC221406

A1BG (Myc-DDK-tagged)-Human alpha-1-B glycoprotein (A1BG)

Product Images

Circular map for RC221406

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  Western blot validation of overexpression lysate (Cat# LY408945) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC221406 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified A1BG protein (Cat# TP321406). The protein was produced from HEK293T cells transfected with A1BG cDNA clone (Cat# RC221406) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: A1BG
• Synonyms: A1B; ABG; GAB; HYST2477
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC221406 representing NM_130786
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCATGCTCGTGGTCTTTCTCTTGCTGTGGGGTGTCACCTGGGGCCCAGTGACAGAAGCAGCCATAT
  TTTATGAGACGCAGCCCAGCCTGTGGGCAGAGTCCGAATCACTGCTGAAACCCTTGGCCAATGTGACGCT
  GACGTGCCAGGCCCACCTGGAGACTCCAGACTTCCAGCTGTTCAAGAATGGGGTGGCCCAGGAGCCTGTG
  CACCTTGACTCACCTGCCATCAAGCACCAGTTCCTGCTGACGGGTGACACCCAGGGCCGCTACCGCTGCC
  GCTCGGGCTTGTCCACAGGATGGACCCAGCTGAGCAAGCTCCTGGAGCTGACAGGGCCAAAGTCCTTGCC
  TGCTCCCTGGCTCTCGATGGCGCCAGTGTCCTGGATCACCCCCGGCCTGAAAACAACAGCAGTGTGCCGA
  GGTGTGCTGCGGGGTGTGACTTTTCTGCTGAGGCGGGAGGGCGACCATGAGTTTCTGGAGGTGCCTGAGG
  CCCAGGAGGATGTGGAGGCCACCTTTCCAGTCCATCAGCCTGGCAACTACAGCTGCAGCTACCGGACCGA
  TGGGGAAGGCGCCCTCTCTGAGCCCAGCGCTACTGTGACCATTGAGGAGCTCGCTGCACCACCACCGCCT
  GTGCTGATGCACCATGGAGAGTCCTCCCAGGTCCTGCACCCTGGCAACAAGGTGACCCTCACCTGCGTGG
  CTCCCCTGAGTGGAGTGGACTTCCAGCTACGGCGCGGGGAGAAAGAGCTGCTGGTACCCAGGAGCAGCAC
  CAGCCCAGATCGCATCTTCTTTCACCTGAACGCGGTGGCCCTGGGGGATGGAGGTCACTACACCTGCCGC
  TACCGGCTGCATGACAACCAAAACGGCTGGTCCGGGGACAGCGCGCCGGTCGAGCTGATTCTGAGCGATG
  AGACGCTGCCCGCGCCGGAGTTCTCCCCGGAGCCGGAGTCCGGCAGGGCCTTGCGGCTGCGGTGCCTGGC
  GCCCCTGGAGGGCGCGCGCTTCGCCCTGGTGCGCGAGGACAGGGGCGGGCGCCGCGTGCACCGTTTCCAG
  AGCCCCGCTGGGACCGAGGCGCTCTTCGAGCTGCACAACATTTCCGTGGCTGACTCCGCCAACTACAGCT
  GCGTCTACGTGGACCTGAAGCCGCCTTTCGGGGGCTCCGCGCCCAGCGAGCGCTTGGAGCTGCACGTGGA
  CGGACCCCCTCCCAGGCCTCAGCTCCGGGCGACGTGGAGTGGGGCGGTCCTGGCGGGCCGAGATGCCGTC
  CTGCGCTGCGAGGGACCCATCCCCGACGTCACCTTCGAGCTGCTGCGCGAGGGCGAGACGAAGGCCGTGA
  AGACGGTCCGCACCCCCGGGGCCGCGGCGAACCTCGAGCTGATCTTCGTGGGGCCCCAGCACGCCGGCAA
  CTACAGGTGCCGCTACCGCTCCTGGGTGCCCCACACCTTCGAATCGGAGCTCAGCGACCCTGTGGAGCTC
  CTGGTGGCAGAAAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC221406 representing NM_130786
  Red=Cloning site Green=Tags(s)
  
  MSMLVVFLLLWGVTWGPVTEAAIFYETQPSLWAESESLLKPLANVTLTCQAHLETPDFQLFKNGVAQEPV
  HLDSPAIKHQFLLTGDTQGRYRCRSGLSTGWTQLSKLLELTGPKSLPAPWLSMAPVSWITPGLKTTAVCR
  GVLRGVTFLLRREGDHEFLEVPEAQEDVEATFPVHQPGNYSCSYRTDGEGALSEPSATVTIEELAAPPPP
  VLMHHGESSQVLHPGNKVTLTCVAPLSGVDFQLRRGEKELLVPRSSTSPDRIFFHLNAVALGDGGHYTCR
  YRLHDNQNGWSGDSAPVELILSDETLPAPEFSPEPESGRALRLRCLAPLEGARFALVREDRGGRRVHRFQ
  SPAGTEALFELHNISVADSANYSCVYVDLKPPFGGSAPSERLELHVDGPPPRPQLRATWSGAVLAGRDAV
  LRCEGPIPDVTFELLREGETKAVKTVRTPGAAANLELIFVGPQHAGNYRCRYRSWVPHTFESELSDPVEL
  LVAES
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_130786
• ORF Size: 1485 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_130786.4
• RefSeq Size: 3386 bp
• RefSeq ORF: 1488 bp
• Locus ID: 1
• UniProt ID: P04217
• Cytogenetics: 19q13.43
• Domains: ig, IGc2, IG
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein
• MW: 51.9 kDa
• Gene Summary: The protein encoded by this gene is a plasma glycoprotein of unknown function. The protein shows sequence similarity to the variable regions of some immunoglobulin supergene family member proteins. [provided by RefSeq, Jul 2008]