MIB1 (NM_020774) Human Tagged ORF Clone

CAT#: RC221377

MIB1 (Myc-DDK-tagged)-Human mindbomb homolog 1 (Drosophila) (MIB1)

Product Images

Circular map for RC221377

• 

  Western blot validation of overexpression lysate (Cat# LY412329) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC221377 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified MIB1 protein (Cat# TP321377). The protein was produced from HEK293T cells transfected with MIB1 cDNA clone (Cat# RC221377) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MIB1
• Synonyms: DIP-1; DIP1; LVNC7; MIB; ZZANK2; ZZZ6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC221377 representing NM_020774
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTAACTCCCGGAATAACCGGGTGATGGTGGAAGGGGTTGGCGCTCGGGTAGTGCGCGGCCCGGACT
  GGAAGTGGGGGAAGCAGGACGGCGGCGAGGGCCATGTGGGCACCGTCCGGAGCTTCGAGAGCCCCGAGGA
  GGTGGTGGTAGTGTGGGACAACGGCACAGCTGCCAACTACCGCTGCTCCGGGGCTTACGACCTCCGCATC
  CTGGACAGCGCGCCCACCGGCATCAAGCATGATGGAACCATGTGTGATACCTGCCGCCAGCAACCAATCA
  TTGGCATTCGATGGAAGTGTGCAGAGTGTACAAATTATGATTTGTGCACAGTGTGTTATCATGGAGATAA
  ACATCATTTAAGACATCGCTTTTACCGAATTACTACACCGGGAAGTGAGAGGGTTCTGTTAGAGTCTCGT
  AGGAAATCTAAGAAGATTACAGCCAGAGGAATCTTTGCAGGTGCCAGAGTGGTGCGAGGAGTGGACTGGC
  AGTGGGAAGATCAAGATGGAGGAAATGGACGTAGGGGAAAGGTAACAGAAATCCAGGACTGGAGTGCATC
  AAGCCCACATAGCGCAGCATATGTCCTCTGGGATAATGGTGCTAAGAACCTTTACAGAGTTGGCTTTGAG
  GGCATGTCTGATCTGAAATGTGTCCAGGATGCCAAGGGAGGTTCTTTCTACAGAGATCACTGCCCTGTGC
  TAGGTGAGCAGAATGGCAACAGGAATCCTGGTGGATTGCAGATTGGTGACCTGGTAAATATAGATCTCGA
  CCTCGAAATTGTACAGTCTTTGCAGCATGGTCATGGAGGATGGACTGATGGAATGTTTGAGACTTTAACT
  ACAACTGGAACTGTTTGTGGCATTGATGAAGATCATGACATTGTAGTACAGTATCCAAGTGGCAATAGGT
  GGACCTTCAATCCTGCTGTTCTCACTAAAGCGAACATTGTCCGAAGTGGAGATGCTGCTCAGGGTGCAGA
  AGGAGGCACCTCGCAGTTTCAAGTGGGTGATCTTGTACAAGTTTGTTATGACCTGGAACGAATTAAACTT
  CTACAAAGAGGACATGGAGAATGGGCTGAAGCGATGCTTCCAACTTTAGGTAAAGTTGGCCGAGTACAAC
  AGATTTATTCAGACAGTGATTTAAAGGTGGAAGTTTGTGGAACATCTTGGACATACAATCCAGCAGCAGT
  TTCCAAGGTGGCATCTGCAGGATCAGCCATTAGCAATGCATCTGGTGAAAGACTCTCACAACTCCTGAAG
  AAATTATTTGAAACCCAAGAATCTGGTGACCTCAATGAAGAATTAGTTAAGGCTGCTGCCAATGGAGATG
  TTGCTAAAGTGGAAGATTTGCTTAAAAGACCAGATGTGGATGTAAATGGGCAATGTGCTGGCCACACAGC
  TATGCAAGCTGCTAGTCAGAATGGACATGTTGACATTTTGAAGTTACTTTTGAAGCAAAACGTGGATGTC
  GAAGCAGAGGATAAAGATGGTGATAGAGCAGTTCACCATGCAGCTTTTGGAGATGAAGGCGCTGTTATAG
  AAGTACTACATCGAGGTAGTGCTGATTTGAATGCTCGAAACAAGCGCCGACAGACACCACTTCATATTGC
  TGTCAATAAAGGTCATCTTCAAGTTGTGAAGACTTTATTGGACTTTGGCTGTCATCCCAGTCTCCAGGAT
  TCTGAAGGTGATACCCCTCTTCATGATGCAATAAGTAAGAAACGTGATGATATCCTAGCAGTTCTTTTGG
  AAGCTGGAGCAGATGTTACCATCACAAACAATAATGGATTTAATGCTCTGCATCATGCTGCACTAAGGGG
  AAATCCCAGTGCAATGCGTGTTTTACTATCTAAATTACCAAGACCATGGATTGTGGATGAGAAGAAAGAT
  GATGGTTATACTGCCTTACATCTGGCTGCCCTTAATAATCACGTAGAAGTGGCTGAACTGTTGGTACATC
  AGGGTAATGCAAACCTGGATATCCAGAATGTGAACCAACAAACTGCCCTACACCTTGCTGTTGAACGACA
  GCATACCCAGATTGTTAGGCTTTTGGTCCGTGCAGGTGCCAAGCTTGATATTCAGGATAAGGATGGGGAT
  ACTCCTTTGCATGAAGCTCTAAGGCATCACACTTTGTCTCAGCTACGTCAGCTCCAAGATATGCAAGATG
  TGGGGAAGGTGGATGCTGCCTGGGAGCCATCCAAAAACACGTTAATAATGGGACTTGGTACCCAGGGGGC
  AGAGAAGAAGAGTGCAGCATCTATTGCCTGTTTCTTGGCAGCCAATGGTGCTGACCTGAGCATTCGAAAT
  AAGAAGGGTCAATCGCCACTTGATCTCTGTCCTGATCCGAATCTCTGCAAAGCACTGGCAAAGTGTCATA
  AGGAAAAAGTCAGTGGTCAAGTGGGTTCTCGGAGTCCTTCTATGATTAGTAATGATTCTGAAACCTTAGA
  AGAGTGTATGGTGTGCTCAGATATGAAGAGAGATACTCTTTTTGGTCCATGTGGACATATTGCTACCTGT
  TCTTTATGTTCTCCACGTGTCAAGAAATGCCTCATCTGTAAAGAACAGGTTCAATCCAGGACAAAGATTG
  AAGAATGTGTGGTATGCTCTGACAAGAAAGCAGCTGTTCTTTTTCAACCCTGTGGCCACATGTGTGCTTG
  TGAGAACTGTGCTAACCTGATGAAAAAGTGTGTGCAGTGTCGAGCAGTAGTTGAACGAAGAGTGCCTTTC
  ATTATGTGCTGTGGAGGGAAAAGTTCAGAAGATGCCACTGATGATATCTCAAGTGGGAATATTCCAGTAT
  TACAAAAGGACAAGGATAATACCAATGTCAATGCAGATGTGCAAAAGTTGCAGCAACAGTTACAAGACAT
  TAAAGAGCAGACAATGTGCCCTGTGTGTCTAGATCGTCTGAAGAATATGATTTTCCTTTGTGGTCACGGA
  ACCTGTCAACTCTGTGGAGACCGCATGAGTGAATGTCCTATCTGTCGCAAGGCTATTGAACGAAGGATTC
  TTTTGTAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC221377 representing NM_020774
  Red=Cloning site Green=Tags(s)
  
  MSNSRNNRVMVEGVGARVVRGPDWKWGKQDGGEGHVGTVRSFESPEEVVVVWDNGTAANYRCSGAYDLRI
  LDSAPTGIKHDGTMCDTCRQQPIIGIRWKCAECTNYDLCTVCYHGDKHHLRHRFYRITTPGSERVLLESR
  RKSKKITARGIFAGARVVRGVDWQWEDQDGGNGRRGKVTEIQDWSASSPHSAAYVLWDNGAKNLYRVGFE
  GMSDLKCVQDAKGGSFYRDHCPVLGEQNGNRNPGGLQIGDLVNIDLDLEIVQSLQHGHGGWTDGMFETLT
  TTGTVCGIDEDHDIVVQYPSGNRWTFNPAVLTKANIVRSGDAAQGAEGGTSQFQVGDLVQVCYDLERIKL
  LQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVEVCGTSWTYNPAAVSKVASAGSAISNASGERLSQLLK
  KLFETQESGDLNEELVKAAANGDVAKVEDLLKRPDVDVNGQCAGHTAMQAASQNGHVDILKLLLKQNVDV
  EAEDKDGDRAVHHAAFGDEGAVIEVLHRGSADLNARNKRRQTPLHIAVNKGHLQVVKTLLDFGCHPSLQD
  SEGDTPLHDAISKKRDDILAVLLEAGADVTITNNNGFNALHHAALRGNPSAMRVLLSKLPRPWIVDEKKD
  DGYTALHLAALNNHVEVAELLVHQGNANLDIQNVNQQTALHLAVERQHTQIVRLLVRAGAKLDIQDKDGD
  TPLHEALRHHTLSQLRQLQDMQDVGKVDAAWEPSKNTLIMGLGTQGAEKKSAASIACFLAANGADLSIRN
  KKGQSPLDLCPDPNLCKALAKCHKEKVSGQVGSRSPSMISNDSETLEECMVCSDMKRDTLFGPCGHIATC
  SLCSPRVKKCLICKEQVQSRTKIEECVVCSDKKAAVLFQPCGHMCACENCANLMKKCVQCRAVVERRVPF
  IMCCGGKSSEDATDDISSGNIPVLQKDKDNTNVNADVQKLQQQLQDIKEQTMCPVCLDRLKNMIFLCGHG
  TCQLCGDRMSECPICRKAIERRILLY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020774
• ORF Size: 3018 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020774.4
• RefSeq Size: 9308 bp
• RefSeq ORF: 3021 bp
• Locus ID: 57534
• UniProt ID: Q86YT6
• Cytogenetics: 18q11.2
• Protein Families: Druggable Genome
• MW: 110 kDa
• Gene Summary: This gene encodes a protein containing multiple ankyrin repeats and RING finger domains that functions as an E3 ubiquitin ligase. The encoded protein positively regulates Notch signaling by ubiquitinating the Notch receptors, thereby facilitating their endocytosis. This protein may also promote the ubiquitination and degradation of death-associated protein kinase 1 (DAPK1). [provided by RefSeq, Jun 2013]