ANKRD55 (NM_024669) Human Tagged ORF Clone

CAT#: RC221211

ANKRD55 (Myc-DDK-tagged)-Human ankyrin repeat domain 55 (ANKRD55)

Product Images

Western blot validation of overexpression lysate (Cat# LY411185) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC221211 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ANKRD55
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC221211 representing NM_024669
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGAGACAGGCTACCATGGATTTCAGCACCCCTTCTGTGTTTGATCAGCAAAGAGGTGACTCATCTG
  AGGAAGTTGACCTGACCATGGTTTATCAAGCAGCCTCTAATGGAGATGTCAATGCTCTGACTGCAGTGAT
  TCGGGAAGACCCTTCTATCCTAGAATGCTGTGACAGTGAAGGATGCACGCCCTTGATGCATGCGGTTTCT
  GGACGTCAAGCGGACACAGTGAAGCTGCTGTTGAAGATGGGAGCCAATATTAACATGCAGGATGCTTATG
  GCCGCACAAGTTTATGCCTGGCCACCTACCTGGGCTGGCTTGAAGGCTGTGTGAGTCTACTCAGAAACGG
  TGCCAAGCACAATATCCCAGATAAAAATGGCCGCCTGCCACTGCATGCTGCCACTGCTGAGCCCGATATG
  AGGCTCCTCACGGTCCTGTTGCAACAGTCGAACATCAGCGAGATTAATCACCAGGACAATGAGGGAATGA
  CACCACTCCACTGGGCGGCTTTCCACAACCAGCCTCAACACACACAAATGCTGCTGAAGAAGGGGGCAGA
  CCCCACCCTTGTGGATAAAGACTTTAAAACCGCTCTCCACTGGGCAGTCCAGAGTGGAAATAGGATTCTG
  TGCTCCATCATTCTGAGCCATCACCAGGGGCCGTCCATAATCAACTATGATGATGAGAGTGGGAAGACAT
  GTGTACATATCGCAGCGGCAGCGGGCTTCAGCGATATTATTCATGAGCTGGCAAGAGTCCCTGAGTGTAA
  CCTGCAGGCTCTGGATGTGGATGACAGGACACCTCTGCACTGGGCTGCAGCTGCAGGGAAGGCCGAATGT
  GTCCAGTCACTGCTGGAGTTGGGAATGGACAGCAACCTGCGGGACATCAATGAGAGCACGCCCTTGGCCT
  ATGCCCTGTACTGCGGTCACACGGCGTGTGTCAAACTCCTCTCCCAAGAGAGCAGAACAGAGCCTACTCG
  ACCCCCTCCCTCCCAGAGCAGTCGGCCCCAGAAGAAGGAGAGACGGTTCAACGTGCTCAACCAAATATTC
  TGCAAAAACAAGAAAGAAGAGCAGAGAGCCCATCAGAAGGATCCCAGCAGGGACCGATACAGAGAGGAGG
  ACACCTCAGAAGTCAATGACATCATCACCACCTTTGATAGCATCGTGGGTACCAACTGCCAAGAACAGCC
  TGGTGATCAGGTGGCTATGGTTGAATTTAAGAAGAAAACCTCAGACAATTCAAAATATCTCTTACCAGAA
  AAGAAACCGCTGGCCCGTAAGGGGCTTCCACCAATCAGAACGCAGAGTCTCCCACCCATCACCCTGGGCA
  ATAACTTCCTAACAGCCTCCCATAGGGCCACTTCCCATGCAGGCCTGAGCTCTGCTCCTCATCATATGGC
  CCAGCGATCTCAGAAAAGTCGAAGTGAGCAGGATTTATTAAATAACAGAACTGGCTGCCAGATGTTACTA
  GATAACCCCTGGAAGAGTGATTCTAATCAGGTATTTTCCTACAAAGTTTGGACTGTGTCTTCTTCTGATA
  AGCTGCTGGACAGATTGCTCAGTGTCCGGCCTGGTCACCAAGAGGTCTCCGTGCCACCACACCTTCGCCA
  TCTACATAATCCATCATCAGGACAAAATTTTCAGCATCTTTCCCCAAACAGACACAAAATCAGGGATCTT
  CCTTTCACTCGGAACAACCTAGCTCCCCTACCAGATCAAAAATTTCTATCTGGAGAACCTCTGCGGACAA
  ACCGAGTGCTTCCTGCAATTCCAAGTCAACGAAGACACAGCACAGCAGCAGAAGAGAGTGAACATTCTGC
  CAACCCCACCAGTGATGAAAAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC221211 representing NM_024669
  Red=Cloning site Green=Tags(s)
  
  MMRQATMDFSTPSVFDQQRGDSSEEVDLTMVYQAASNGDVNALTAVIREDPSILECCDSEGCTPLMHAVS
  GRQADTVKLLLKMGANINMQDAYGRTSLCLATYLGWLEGCVSLLRNGAKHNIPDKNGRLPLHAATAEPDM
  RLLTVLLQQSNISEINHQDNEGMTPLHWAAFHNQPQHTQMLLKKGADPTLVDKDFKTALHWAVQSGNRIL
  CSIILSHHQGPSIINYDDESGKTCVHIAAAAGFSDIIHELARVPECNLQALDVDDRTPLHWAAAAGKAEC
  VQSLLELGMDSNLRDINESTPLAYALYCGHTACVKLLSQESRTEPTRPPPSQSSRPQKKERRFNVLNQIF
  CKNKKEEQRAHQKDPSRDRYREEDTSEVNDIITTFDSIVGTNCQEQPGDQVAMVEFKKKTSDNSKYLLPE
  KKPLARKGLPPIRTQSLPPITLGNNFLTASHRATSHAGLSSAPHHMAQRSQKSRSEQDLLNNRTGCQMLL
  DNPWKSDSNQVFSYKVWTVSSSDKLLDRLLSVRPGHQEVSVPPHLRHLHNPSSGQNFQHLSPNRHKIRDL
  PFTRNNLAPLPDQKFLSGEPLRTNRVLPAIPSQRRHSTAAEESEHSANPTSDEN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_024669
• ORF Size: 1842 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_024669.3
• RefSeq Size: 2502 bp
• RefSeq ORF: 1845 bp
• Locus ID: 79722
• UniProt ID: Q3KP44
• Cytogenetics: 5q11.2
• MW: 68.2 kDa