SLC10A2 (NM_000452) Human Tagged ORF Clone

CAT#: RC221202

SLC10A2 (Myc-DDK-tagged)-Human solute carrier family 10 (sodium/bile acid cotransporter family), member 2 (SLC10A2)

Product Images

Circular map for RC221202

• 

  Western blot validation of overexpression lysate (Cat# LY424708) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC221202 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SLC10A2
• Synonyms: ASBT; IBAT; ISBT; NTCP2; PBAM
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC221202 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATGATCCGAACAGCTGTGTGGACAATGCAACAGTTTGCTCTGGTGCATCCTGTGTGGTACCTGAGA
  GCAATTTCAATAACATCCTAAGTGTGGTCCTAAGTACGGTGCTGACCATCCTGTTGGCCTTGGTGATGTT
  CTCCATGGGATGCAACGTGGAAATCAAGAAATTTCTAGGGCACATAAAGCGGCCGTGGGGCATTTGTGTT
  GGCTTCCTCTGTCAGTTTGGAATCATGCCCCTCACAGGATTCATCCTGTCGGTGGCCTTTGACATCCTCC
  CGCTCCAGGCCGTAGTGGTGCTCATTATAGGATGCTGCCCTGGAGGAACTGCCTCCAATATCTTGGCCTA
  TTGGGTCGATGGCGACATGGACCTGAGCGTCAGCATGACCACATGCTCCACACTGCTTGCCCTCGGAATG
  ATGCCGCTGTGCCTCCTTATCTATACCAAAATGTGGGTCGACTCTGGGAGCATCGTAATTCCCTATGATA
  ACATAGGTACATCTCTGGTTGCTCTCGTTGTTCCTGTTTCCATTGGAATGTTTGTTAATCACAAATGGCC
  CCAAAAAGCAAAGATCATACTTAAAATTGGGTCCATCGCGGGCGCCATCCTCATTGTGCTCATAGCTGTG
  GTTGGAGGAATATTGTACCAAAGCGCCTGGATCATTGCTCCCAAACTGTGGATTATAGGAACAATATTTC
  CTGTGGCGGGTTACTCCCTGGGGTTTCTTCTGGCTAGAATTGCTGGTCTACCCTGGTACAGGTGCCGAAC
  GGTTGCTTTTGAAACGGGGATGCAGAACACGCAGCTATGTTCCACCATCGTTCAGCTCTCCTTCACTCCT
  GAGGAGCTCAATGTCGTATTCACCTTCCCGCTCATCTACAGCATTTTCCAGCTCGCCTTTGCCGCAATAT
  TCTTAGGATTTTATGTGGCATACAAGAAATGTCATGGAAAAAACAAGGCAGAAATTCCAGAGAGCAAAGA
  AAATGGAACGGAGCCAGAGTCATCGTTTTATAAGGCAAATGGAGGATTTCAACCTGACGAAAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC221202 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MNDPNSCVDNATVCSGASCVVPESNFNNILSVVLSTVLTILLALVMFSMGCNVEIKKFLGHIKRPWGICV
  GFLCQFGIMPLTGFILSVAFDILPLQAVVVLIIGCCPGGTASNILAYWVDGDMDLSVSMTTCSTLLALGM
  MPLCLLIYTKMWVDSGSIVIPYDNIGTSLVALVVPVSIGMFVNHKWPQKAKIILKIGSIAGAILIVLIAV
  VGGILYQSAWIIAPKLWIIGTIFPVAGYSLGFLLARIAGLPWYRCRTVAFETGMQNTQLCSTIVQLSFTP
  EELNVVFTFPLIYSIFQLAFAAIFLGFYVAYKKCHGKNKAEIPESKENGTEPESSFYKANGGFQPDEK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_000452
• ORF Size: 1044 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000452.2, NP_000443.1
• RefSeq Size: 3779 bp
• RefSeq ORF: 1047 bp
• Locus ID: 6555
• UniProt ID: Q12908
• Cytogenetics: 13q33.1
• Protein Families: Druggable Genome, Transmembrane
• MW: 37.7 kDa
• Gene Summary: This gene encodes a sodium/bile acid cotransporter. This transporter is the primary mechanism for uptake of intestinal bile acids by apical cells in the distal ileum. Bile acids are the catabolic product of cholesterol metabolism, so this protein is also critical for cholesterol homeostasis. Mutations in this gene cause primary bile acid malabsorption (PBAM); muatations in this gene may also be associated with other diseases of the liver and intestines, such as familial hypertriglyceridemia (FHTG). [provided by RefSeq, Mar 2010]