CNN2 (NM_004368) Human Tagged ORF Clone

CAT#: RC221104

CNN2 (Myc-DDK-tagged)-Human calponin 2 (CNN2), transcript variant 1

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY CNN2 (Cat# RC221104, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CNN2(Cat# TA503688). Positive lysates LY418024 (100ug) and LC418024 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY418024) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC221104 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified CNN2 protein (Cat# TP321104). The protein was produced from HEK293T cells transfected with CNN2 cDNA clone (Cat# RC221104) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CNN2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC221104 representing NM_004368
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCTCCACGCAGTTCAACAAGGGCCCCTCGTACGGGCTGTCGGCCGAGGTCAAGAACCGGCTCCTGT
  CCAAATATGACCCCCAGAAGGAGGCAGAGCTCCGCACCTGGATCGAGGGACTCACCGGCCTCTCCATCGG
  CCCCGACTTCCAGAAGGGCCTGAAGGATGGAACTATCTTATGCACACTCATGAACAAGCTACAGCCGGGC
  TCCGTCCCCAAGATCAACCGCTCCATGCAGAACTGGCACCAGCTAGAAAACCTGTCCAACTTCATCAAGG
  CCATGGTCAGCTACGGCATGAACCCTGTGGACCTGTTCGAGGCCAACGACCTGTTTGAGAGTGGGAACAT
  GACGCAGGTGCAGGTGTCTCTTCTCGCCCTGGCGGGGAAGGCCAAGACTAAGGGGCTGCAGAGCGGGGTG
  GACATTGGCGTCAAGTACTCGGAGAAGCAGGAGCGGAATTTCGACGATGCCACCATGAAGGCTGGCCAGT
  GCGTCATCGGGCTGCAGATGGGCACCAACAAATGCGCCAGCCAGTCGGGCATGACTGCCTACGGCACGAG
  AAGGCATCTCTATGACCCCAAGAACCATATCCTGCCCCCCATGGACCACTCGACCATCAGCCTCCAGATG
  GGCACGAACAAGTGCGCCAGCCAGGTGGGCATGACGGCTCCCGGGACCCGGCGGCACATCTATGATACCA
  AGCTGGGAACCGACAAGTGTGACAACTCCTCCATGTCCCTGCAGATGGGCTACACGCAGGGCGCCAACCA
  GAGCGGCCAGGTCTTCGGCCTGGGCCGGCAGATATATGACCCCAAGTACTGCCCGCAAGGCACAGTGGCC
  GATGGGGCTCCCTCGGGCACCGGCGACTGCCCGGACCCGGGGGAGGTCCCTGAATATCCCCCTTACTACC
  AGGAGGAGGCCGGCTAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC221104 representing NM_004368
  Red=Cloning site Green=Tags(s)
  
  MSSTQFNKGPSYGLSAEVKNRLLSKYDPQKEAELRTWIEGLTGLSIGPDFQKGLKDGTILCTLMNKLQPG
  SVPKINRSMQNWHQLENLSNFIKAMVSYGMNPVDLFEANDLFESGNMTQVQVSLLALAGKAKTKGLQSGV
  DIGVKYSEKQERNFDDATMKAGQCVIGLQMGTNKCASQSGMTAYGTRRHLYDPKNHILPPMDHSTISLQM
  GTNKCASQVGMTAPGTRRHIYDTKLGTDKCDNSSMSLQMGYTQGANQSGQVFGLGRQIYDPKYCPQGTVA
  DGAPSGTGDCPDPGEVPEYPPYYQEEAGY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004368
• ORF Size: 927 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004368.1
• RefSeq Size: 2478 bp
• RefSeq ORF: 930 bp
• Locus ID: 1265
• UniProt ID: Q99439
• Cytogenetics: 19p13.3
• Domains: calponin, CH
• MW: 33.5 kDa
• Gene Summary: The protein encoded by this gene, which can bind actin, calmodulin, troponin C, and tropomyosin, may function in the structural organization of actin filaments. The encoded protein could play a role in smooth muscle contraction and cell adhesion. Several pseudogenes of this gene have been identified, and are present on chromosomes 1, 2, 3, 6, 9, 11, 13, 15, 16, 21 and 22. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2015]