APOBEC3A (NM_145699) Human Tagged ORF Clone
APOBEC3A (Myc-DDK-tagged)-Human apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3A (APOBEC3A), transcript variant 1
|Product Name||APOBEC3A (NM_145699) Human Tagged ORF Clone|
|Type||Human Tagged ORF Clone|
|Synonyms||A3A; ARP3; bK150C2.1; PHRBN|
|E. coli Selection||Kanamycin (25 ug/mL)|
>RC220995 representing NM_145699
Red=Cloning site Blue=ORF Green=Tags(s)
>RC220995 representing NM_145699
Red=Cloning site Green=Tags(s)
Sequencher program is needed, download here.
|Restriction Sites||SgfI-MluI Cloning Scheme for this gene Plasmid Map|
|ORF Size||597 bp|
|OTI Disclaimer||The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info|
|OTI Annotation||This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.|
|Product Components||The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.|
|Reconstitution||1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
|RefSeq||NM_145699.1, NM_145699.2, NM_145699.3, NP_663745.1|
|RefSeq Size||1349 bp|
|RefSeq ORF||600 bp|
|Gene Summary||This gene is a member of the cytidine deaminase gene family. It is one of seven related genes or pseudogenes found in a cluster, thought to result from gene duplication, on chromosome 22. Members of the cluster encode proteins that are structurally and functionally related to the C to U RNA-editing cytidine deaminase APOBEC1. The protein encoded by this gene lacks the zinc binding activity of other family members. The protein plays a role in immunity, by restricting transmission of foreign DNA such as viruses. One mechanism of foreign DNA restriction is deamination of foreign double-stranded DNA cytidines to uridines, which leads to DNA degradation. However, other mechanisms are also thought to be involved, as anti-viral effect is not dependent on deaminase activity. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2012]|
|The use of this cDNA Clones has been cited in the following citations:|
Transient overexpression of exogenous APOBEC3A causes C-to-U RNA editing of thousands of genes
,Sharma, S;Patnaik, SK;Kemer, Z;Baysal, BE;,
RNA Biol May 2016
,PubMed ID 27149507
APOBEC3A cytidine deaminase induces RNA editing in monocytes and macrophages
,Sharma, S;Patnaik, SK;Thomas Taggart, R;Kannisto, ED;Enriquez, SM;Gollnick, P;Baysal, BE;,
Nat Commun Apr 2015
,PubMed ID 25898173
|cDNA Clone Resources|
|SC306262||APOBEC3A (untagged)-Human apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3A (APOBEC3A), transcript variant 1||
|RG220995||APOBEC3A (GFP-tagged) - Human apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3A (APOBEC3A), transcript variant 1||
|RC220995L1||Lenti ORF clone of Human apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3A (APOBEC3A), transcript variant 1, Myc-DDK-tagged||
|RC220995L2||Lenti ORF clone of Human apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3A (APOBEC3A), transcript variant 1, mGFP tagged||
|RC220995L3||Lenti ORF clone of Human apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3A (APOBEC3A), transcript variant 1, Myc-DDK-tagged||
|RC220995L4||Lenti ORF clone of Human apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3A (APOBEC3A), transcript variant 1, mGFP tagged||