Phospholipase A2 (PLA2G4A) (NM_024420) Human Tagged ORF Clone

CAT#: RC220972

PLA2G4A (Myc-DDK-tagged)-Human phospholipase A2, group IVA (cytosolic, calcium-dependent) (PLA2G4A)

Product Images

Western blot validation of overexpression lysate (Cat# LY402988) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC220972 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified PLA2G4A protein (Cat# TP320972). The protein was produced from HEK293T cells transfected with PLA2G4A cDNA clone (Cat# RC220972) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PLA2G4A
• Synonyms: cPLA2; cPLA2-alpha; GURDP; PLA2G4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC220972 representing NM_024420
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCATTTATAGATCCTTACCAGCACATTATAGTGGAGCACCAGTATTCCCACAAGTTTACGGTAGTGG
  TGTTACGTGCCACCAAAGTGACAAAGGGGGCCTTTGGTGACATGCTTGATACTCCAGATCCCTATGTGGA
  ACTTTTTATCTCTACAACCCCTGACAGCAGGAAGAGAACAAGACATTTCAATAATGACATAAACCCTGTG
  TGGAATGAGACCTTTGAATTTATTTTGGATCCTAATCAGGAAAATGTTTTGGAGATTACGTTAATGGATG
  CCAATTATGTCATGGATGAAACTCTAGGGACAGCAACATTTACTGTATCTTCTATGAAGGTGGGAGAAAA
  GAAAGAAGTTCCTTTTATTTTCAACCAAGTCACTGAAATGGTTCTAGAAATGTCTCTTGAAGTTTGCTCA
  TGCCCAGACCTACGATTTAGTATGGCTCTGTGTGATCAGGAGAAGACTTTCAGACAACAGAGAAAAGAAC
  ACATAAGGGAGAGCATGAAGAAACTCTTGGGTCCAAAGAATAGTGAAGGATTGCATTCTGCACGTGATGT
  GCCTGTGGTAGCCATATTGGGTTCAGGTGGGGGTTTCCGAGCCATGGTGGGATTCTCTGGTGTGATGAAG
  GCATTATACGAATCAGGAATTCTGGATTGTGCTACCTACGTTGCTGGTCTTTCTGGCTCCACCTGGTATA
  TGTCAACCTTGTATTCTCACCCTGATTTTCCAGAGAAAGGGCCAGAGGAGATTAATGAAGAACTAATGAA
  AAATGTTAGCCACAATCCCCTTTTACTTCTCACACCACAGAAAGTTAAAAGATATGTTGAGTCTTTATGG
  AAGAAGAAAAGCTCTGGACAACCTGTCACCTTTACTGATATCTTTGGGATGTTAATAGGAGAAACACTAA
  TTCATAATAGAATGAATACTACTCTGAGCAGTTTGAAGGAAAAAGTTAATACTGCACAATGCCCTTTACC
  TCTTTTCACCTGTCTTCATGTCAAACCTGACGTTTCAGAGCTGATGTTTGCAGATTGGGTTGAATTTAGT
  CCATACGAAATTGGCATGGCTAAATATGGTACTTTTATGGCTCCCGACTTATTTGGAAGCAAATTTTTTA
  TGGGAACAGTCGTTAAGAAGTATGAAGAAAACCCCTTGCATTTCTTAATGGGTGTCTGGGGCAGTGCCTT
  TTCCATATTGTTCAACAGAGTTTTGGGCGTTTCTGGTTCACAAAGCAGAGGCTCCACAATGGAGGAAGAA
  TTAGAAAATATTACCACAAAGCATATTGTGAGTAATGATAGCTCGGACAGTGATGATGAATCACACGAAC
  CCAAAGGCACTGAAAATGAAGATGCTGGAAGTGACTATCAAAGTGATAATCAAGCAAGTTGGATTCATCG
  TATGATAATGGCCTTGGTGAGTGATTCAGCTTTATTCAATACCAGAGAAGGACGTGCTGGGAAGGTACAC
  AACTTCATGCTGGGCTTGAATCTCAATACATCTTATCCACTGTCTCCTTTGAGTGACTTTGCCACACAGG
  ACTCCTTTGATGATGATGAACTGGATGCAGCTGTAGCAGATCCTGATGAATTTGAGCGAATATATGAGCC
  TCTGGATGTCAAAAGTAAAAAGATTCATGTAGTGGACAGTGGGCTCACATTTAACCTGCCGTATCCCTTG
  ATACTGAGACCTCAGAGAGGGGTTGATCTCATAATCTCCTTTGACTTTTCTGCAAGGCCAAGTGACTCTA
  GTCCTCCGTTCAAGGAACTTCTACTTGCAGAAAAGTGGGCTAAAATGAACAAGCTCCCCTTTCCAAAGAT
  TGATCCTTATGTGTTTGATCGGGAAGGGCTGAAGGAGTGCTATGTCTTTAAACCCAAGAATCCTGATATG
  GAGAAAGATTGCCCAACCATCATCCACTTTGTTCTGGCCAACATCAACTTCAGAAAGTACAAGGCTCCAG
  GTGTTCCAAGGGAAACTGAGGAAGAGAAAGAAATCGCTGACTTTGATATTTTTGATGACCCAGAATCACC
  ATTTTCAACCTTCAATTTTCAATATCCAAATCAAGCATTCAAAAGACTACATGATCTTATGCACTTCAAT
  ACTCTGAACAACATTGATGTGATAAAAGAAGCCATGGTTGAAAGCATTGAATATAGAAGACAGAATCCAT
  CTCGTTGCTCTGTTTCCCTTAGTAATGTTGAGGCAAGAAGATTTTTCAACAAGGAGTTTCTAAGTAAACC
  CAAAGCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC220972 representing NM_024420
  Red=Cloning site Green=Tags(s)
  
  MSFIDPYQHIIVEHQYSHKFTVVVLRATKVTKGAFGDMLDTPDPYVELFISTTPDSRKRTRHFNNDINPV
  WNETFEFILDPNQENVLEITLMDANYVMDETLGTATFTVSSMKVGEKKEVPFIFNQVTEMVLEMSLEVCS
  CPDLRFSMALCDQEKTFRQQRKEHIRESMKKLLGPKNSEGLHSARDVPVVAILGSGGGFRAMVGFSGVMK
  ALYESGILDCATYVAGLSGSTWYMSTLYSHPDFPEKGPEEINEELMKNVSHNPLLLLTPQKVKRYVESLW
  KKKSSGQPVTFTDIFGMLIGETLIHNRMNTTLSSLKEKVNTAQCPLPLFTCLHVKPDVSELMFADWVEFS
  PYEIGMAKYGTFMAPDLFGSKFFMGTVVKKYEENPLHFLMGVWGSAFSILFNRVLGVSGSQSRGSTMEEE
  LENITTKHIVSNDSSDSDDESHEPKGTENEDAGSDYQSDNQASWIHRMIMALVSDSALFNTREGRAGKVH
  NFMLGLNLNTSYPLSPLSDFATQDSFDDDELDAAVADPDEFERIYEPLDVKSKKIHVVDSGLTFNLPYPL
  ILRPQRGVDLIISFDFSARPSDSSPPFKELLLAEKWAKMNKLPFPKIDPYVFDREGLKECYVFKPKNPDM
  EKDCPTIIHFVLANINFRKYKAPGVPRETEEEKEIADFDIFDDPESPFSTFNFQYPNQAFKRLHDLMHFN
  TLNNIDVIKEAMVESIEYRRQNPSRCSVSLSNVEARRFFNKEFLSKPKA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_024420
• ORF Size: 2247 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_024420.2
• RefSeq Size: 2875 bp
• RefSeq ORF: 2250 bp
• Locus ID: 5321
• UniProt ID: P47712
• Cytogenetics: 1q31.1
• Domains: C2, PLA2_B
• Protein Pathways: alpha-Linolenic acid metabolism, Arachidonic acid metabolism, Ether lipid metabolism, Fc epsilon RI signaling pathway, Fc gamma R-mediated phagocytosis, Glycerophospholipid metabolism, GnRH signaling pathway, Linoleic acid metabolism, Long-term depression, MAPK signaling pathway, Metabolic pathways, Vascular smooth muscle contraction, VEGF signaling pathway
• MW: 85 kDa
• Gene Summary: This gene encodes a member of the cytosolic phospholipase A2 group IV family. The enzyme catalyzes the hydrolysis of membrane phospholipids to release arachidonic acid which is subsequently metabolized into eicosanoids. Eicosanoids, including prostaglandins and leukotrienes, are lipid-based cellular hormones that regulate hemodynamics, inflammatory responses, and other intracellular pathways. The hydrolysis reaction also produces lysophospholipids that are converted into platelet-activating factor. The enzyme is activated by increased intracellular Ca(2+) levels and phosphorylation, resulting in its translocation from the cytosol and nucleus to perinuclear membrane vesicles. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2015]