KATNAL1 (NM_001014380) Human Tagged ORF Clone

CAT#: RC220944

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KATNAL1 (Myc-DDK-tagged)-Human katanin p60 subunit A-like 1 (KATNAL1), transcript variant 2



Reconstitution Protocol

USD 457.00

In Stock*

Size
    • 10 ug

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Frequently bought together (4)
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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol KATNAL1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC220944 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAATTTGGCTGAGATTTGTGATAATGCAAAGAAAGGAAGAGAATATGCCCTTCTTGGAAATTACGACT
CATCAATGGTATATTACCAGGGGGTGATGCAGCAGATTCAGAGACATTGCCAGTCAGTCAGAGATCCAGC
TATCAAAGGCAAATGGCAACAGGTTCGGCAGGAATTATTGGAGGAATATGAACAAGTTAAAAGTATTGTC
AGCACTTTAGAAAGTTTTAAAATTGACAAGCCTCCAGATTTCCCTGTGTCCTGTCAAGATGAACCATTTA
GAGATCCTGCTGTTTGGCCACCCCCTGTTCCTGCAGAACACAGAGCTCCACCTCAGATCAGGCGTCCCAA
TCGAGAAGTAAGACCTCTGAGGAAAGAAATGGCAGGAGTAGGAGCCCGGGGACCTGTAGGCCGAGCACAT
CCTATATCAAAGAGTGAAAAGCCTTCTACAAGTAGGGACAAGGACTATAGAGCAAGAGGGAGAGATGACA
AGGGAAGGAAGAATATGCAAGATGGTGCAAGTGATGGTGAAATGCCAAAATTTGATGGTGCTGGTTATGA
TAAGGATCTGGTGGAAGCCCTTGAAAGAGACATTGTATCCAGGAATCCTAGCATTCATTGGGATGACATA
GCAGATCTGGAAGAAGCTAAGAAGTTGCTAAGGGAAGCTGTTGTTCTTCCAATGTGGATGCCTGACTTTT
TCAAAGGGATTAGAAGGCCATGGAAGGGTGTACTGATGGTTGGACCCCCAGGCACTGGTAAAACTATGCT
AGCTAAAGCTGTTGCCACTGAATGTGGTACAACATTCTTCAACGTTTCGTCTTCTACACTGACATCTAAA
TACAGAGGTGAATCTGAGAAGTTAGTTCGTCTGTTGTTTGAGATGGCTAGATTTTATGCCCCTACCACGA
TCTTCATTGATGAGATAGATTCTATCTGCAGTCGAAGAGGAACCTCTGATGAACATGAGGCAAGTCGCAG
GGTCAAGTCTGAACTGCTCATTCAGATGGATGGAGTTGGAGGAGCTTTAGAAAATGATGATCCTTCCAAA
ATGGTTATGGTATTGGCTGCTACTAATTTCCCGTGGGACATTGATGAAGCTTTGCGAAGAAGGTTAGAAA
AAAGGATATATATACCTCTCCCAACAGCAAAAGGAAGAGCTGAGCTTCTGAAGATCAACCTTCGTGAGGT
CGAATTAGATCCTGATATTCAACTGGAAGATATAGCCGAGAAGATTGAGGGCTATTCTGGTGCTGACATC
ACTAATGTTTGCAGGGATGCCTCTTTAATGGCAATGAGACGGCGTATCAATGGCTTAAGTCCAGAAGAAA
TCCGTGCACTTTCTAAAGAGGAACTTCAGATGCCTGTTACCAAAGGAGACTTTGAATTGGCCCTAAAGAA
AATTGCTAAGTCTGTCTCTGCTGCAGACTTGGAGAAGTATGAAAAATGGATGGTTGAATTTGGATCTGCT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC220944 protein sequence
Red=Cloning site Green=Tags(s)

MNLAEICDNAKKGREYALLGNYDSSMVYYQGVMQQIQRHCQSVRDPAIKGKWQQVRQELLEEYEQVKSIV
STLESFKIDKPPDFPVSCQDEPFRDPAVWPPPVPAEHRAPPQIRRPNREVRPLRKEMAGVGARGPVGRAH
PISKSEKPSTSRDKDYRARGRDDKGRKNMQDGASDGEMPKFDGAGYDKDLVEALERDIVSRNPSIHWDDI
ADLEEAKKLLREAVVLPMWMPDFFKGIRRPWKGVLMVGPPGTGKTMLAKAVATECGTTFFNVSSSTLTSK
YRGESEKLVRLLFEMARFYAPTTIFIDEIDSICSRRGTSDEHEASRRVKSELLIQMDGVGGALENDDPSK
MVMVLAATNFPWDIDEALRRRLEKRIYIPLPTAKGRAELLKINLREVELDPDIQLEDIAEKIEGYSGADI
TNVCRDASLMAMRRRINGLSPEEIRALSKEELQMPVTKGDFELALKKIAKSVSAADLEKYEKWMVEFGSA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001014380
ORF Size 1470 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001014380.3
RefSeq Size 7512 bp
RefSeq ORF 1473 bp
Locus ID 84056
UniProt ID Q9BW62
Cytogenetics 13q12.3
MW 55.4 kDa
Gene Summary Regulates microtubule dynamics in Sertoli cells, a process that is essential for spermiogenesis and male fertility. Severs microtubules in an ATP-dependent manner, promoting rapid reorganization of cellular microtubule arrays (By similarity). Has microtubule-severing activity in vitro (PubMed:26929214).[UniProtKB/Swiss-Prot Function]
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