KATNAL1 (NM_001014380) Human Tagged ORF Clone

CAT#: RC220944

KATNAL1 (Myc-DDK-tagged)-Human katanin p60 subunit A-like 1 (KATNAL1), transcript variant 2

Product Images

Circular map for RC220944

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  Western blot validation of overexpression lysate (Cat# LY423055) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC220944 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified KATNAL1 protein (Cat# TP320944). The protein was produced from HEK293T cells transfected with KATNAL1 cDNA clone (Cat# RC220944) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: KATNAL1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC220944 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATTTGGCTGAGATTTGTGATAATGCAAAGAAAGGAAGAGAATATGCCCTTCTTGGAAATTACGACT
  CATCAATGGTATATTACCAGGGGGTGATGCAGCAGATTCAGAGACATTGCCAGTCAGTCAGAGATCCAGC
  TATCAAAGGCAAATGGCAACAGGTTCGGCAGGAATTATTGGAGGAATATGAACAAGTTAAAAGTATTGTC
  AGCACTTTAGAAAGTTTTAAAATTGACAAGCCTCCAGATTTCCCTGTGTCCTGTCAAGATGAACCATTTA
  GAGATCCTGCTGTTTGGCCACCCCCTGTTCCTGCAGAACACAGAGCTCCACCTCAGATCAGGCGTCCCAA
  TCGAGAAGTAAGACCTCTGAGGAAAGAAATGGCAGGAGTAGGAGCCCGGGGACCTGTAGGCCGAGCACAT
  CCTATATCAAAGAGTGAAAAGCCTTCTACAAGTAGGGACAAGGACTATAGAGCAAGAGGGAGAGATGACA
  AGGGAAGGAAGAATATGCAAGATGGTGCAAGTGATGGTGAAATGCCAAAATTTGATGGTGCTGGTTATGA
  TAAGGATCTGGTGGAAGCCCTTGAAAGAGACATTGTATCCAGGAATCCTAGCATTCATTGGGATGACATA
  GCAGATCTGGAAGAAGCTAAGAAGTTGCTAAGGGAAGCTGTTGTTCTTCCAATGTGGATGCCTGACTTTT
  TCAAAGGGATTAGAAGGCCATGGAAGGGTGTACTGATGGTTGGACCCCCAGGCACTGGTAAAACTATGCT
  AGCTAAAGCTGTTGCCACTGAATGTGGTACAACATTCTTCAACGTTTCGTCTTCTACACTGACATCTAAA
  TACAGAGGTGAATCTGAGAAGTTAGTTCGTCTGTTGTTTGAGATGGCTAGATTTTATGCCCCTACCACGA
  TCTTCATTGATGAGATAGATTCTATCTGCAGTCGAAGAGGAACCTCTGATGAACATGAGGCAAGTCGCAG
  GGTCAAGTCTGAACTGCTCATTCAGATGGATGGAGTTGGAGGAGCTTTAGAAAATGATGATCCTTCCAAA
  ATGGTTATGGTATTGGCTGCTACTAATTTCCCGTGGGACATTGATGAAGCTTTGCGAAGAAGGTTAGAAA
  AAAGGATATATATACCTCTCCCAACAGCAAAAGGAAGAGCTGAGCTTCTGAAGATCAACCTTCGTGAGGT
  CGAATTAGATCCTGATATTCAACTGGAAGATATAGCCGAGAAGATTGAGGGCTATTCTGGTGCTGACATC
  ACTAATGTTTGCAGGGATGCCTCTTTAATGGCAATGAGACGGCGTATCAATGGCTTAAGTCCAGAAGAAA
  TCCGTGCACTTTCTAAAGAGGAACTTCAGATGCCTGTTACCAAAGGAGACTTTGAATTGGCCCTAAAGAA
  AATTGCTAAGTCTGTCTCTGCTGCAGACTTGGAGAAGTATGAAAAATGGATGGTTGAATTTGGATCTGCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC220944 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MNLAEICDNAKKGREYALLGNYDSSMVYYQGVMQQIQRHCQSVRDPAIKGKWQQVRQELLEEYEQVKSIV
  STLESFKIDKPPDFPVSCQDEPFRDPAVWPPPVPAEHRAPPQIRRPNREVRPLRKEMAGVGARGPVGRAH
  PISKSEKPSTSRDKDYRARGRDDKGRKNMQDGASDGEMPKFDGAGYDKDLVEALERDIVSRNPSIHWDDI
  ADLEEAKKLLREAVVLPMWMPDFFKGIRRPWKGVLMVGPPGTGKTMLAKAVATECGTTFFNVSSSTLTSK
  YRGESEKLVRLLFEMARFYAPTTIFIDEIDSICSRRGTSDEHEASRRVKSELLIQMDGVGGALENDDPSK
  MVMVLAATNFPWDIDEALRRRLEKRIYIPLPTAKGRAELLKINLREVELDPDIQLEDIAEKIEGYSGADI
  TNVCRDASLMAMRRRINGLSPEEIRALSKEELQMPVTKGDFELALKKIAKSVSAADLEKYEKWMVEFGSA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001014380
• ORF Size: 1470 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001014380.3
• RefSeq Size: 7512 bp
• RefSeq ORF: 1473 bp
• Locus ID: 84056
• UniProt ID: Q9BW62
• Cytogenetics: 13q12.3
• MW: 55.4 kDa
• Gene Summary: Regulates microtubule dynamics in Sertoli cells, a process that is essential for spermiogenesis and male fertility. Severs microtubules in an ATP-dependent manner, promoting rapid reorganization of cellular microtubule arrays (By similarity). Has microtubule-severing activity in vitro (PubMed:26929214).[UniProtKB/Swiss-Prot Function]