p16INK4A(CDKN2A) (NM_000077) Human Tagged ORF Clone

CAT#: RC220937L2

Lenti ORF clone of Human cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4) (CDKN2A), transcript variant 1, mGFP tagged

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  • LentiORF®

USD 660


Availability*
3 Weeks

Size
    • 10 ug

Product images

Specifications

Product Data
Product Name p16INK4A(CDKN2A) (NM_000077) Human Tagged ORF Clone
Symbol CDKN2A
Synonyms ARF; CDK4I; CDKN2; CMM2; INK4; INK4A; MLM; MTS-1; MTS1; P14; P14ARF; P16; P16-INK4A; P16INK4
Vector pLenti-C-mGFP
Sequence Data
ORF Nucleotide Sequence
The ORF insert of this clone is exactly the same as(RC220937).
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
Tag mGFP
ACCN NM_000077
ORF Size 468 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reference Data
RefSeq NM_000077.3, NP_000068
RefSeq Size 1163
RefSeq ORF 471
Locus ID 1029
Cytogenetics 9p21.3
Protein Families Druggable Genome
Protein Pathways Cell cycle, p53 signaling pathway, Pathways in cancer, Pancreatic cancer, Glioma, Melanoma, Bladder cancer, Chronic myeloid leukemia, Non-small cell lung cancer
MW 16.4 kDa
Gene Summary This gene generates several transcript variants which differ in their first exons. At least three alternatively spliced variants encoding distinct proteins have been reported, two of which encode structurally related isoforms known to function as inhibitors of CDK4 kinase. The remaining transcript includes an alternate first exon located 20 Kb upstream of the remainder of the gene; this transcript contains an alternate open reading frame (ARF) that specifies a protein which is structurally unrelated to the products of the other variants. This ARF product functions as a stabilizer of the tumor suppressor protein p53 as it can interact with, and sequester, the E3 ubiquitin-protein ligase MDM2, a protein responsible for the degradation of p53. In spite of the structural and functional differences, the CDK inhibitor isoforms and the ARF product encoded by this gene, through the regulatory roles of CDK4 and p53 in cell cycle G1 progression, share a common functionality in cell cycle G1 control. This gene is frequently mutated or deleted in a wide variety of tumors, and is known to be an important tumor suppressor gene. [provided by RefSeq, Sep 2012]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations