ANK1 (NM_020476) Human Tagged ORF Clone

CAT#: RC220763

ANK1 (Myc-DDK-tagged)-Human ankyrin 1, erythrocytic (ANK1), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ANK1
• Synonyms: ANK; SPH1; SPH2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC220763 representing NM_020476
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCTATTCTGTGGGCTTCCGCGAAGCCGATGCTGCTACCAGCTTTCTGAGAGCAGCAAGATCAGGTA
  ACTTGGACAAAGCTTTGGATCACCTGCGGAATGGGGTAGATATTAACACCTGTAACCAGAATGGGTTGAA
  TGGCTTGCATCTGGCTTCTAAGGAAGGCCATGTGAAAATGGTGGTTGAACTTCTGCACAAAGAAATCATT
  CTAGAAACGACAACCAAGAAGGGGAACACGGCCCTGCACATCGCTGCTCTAGCCGGGCAGGATGAGGTGG
  TCCGGGAGCTTGTCAACTATGGAGCCAACGTCAACGCCCAGTCACAGAAAGGTTTTACACCCCTGTACAT
  GGCAGCACAAGAGAACCACTTGGAAGTGGTTAAGTTTTTACTGGAAAATGGAGCTAACCAGAATGTAGCC
  ACAGAAGACGGCTTCACGCCTCTGGCGGTAGCCCTGCAGCAGGGCCATGAGAACGTCGTCGCGCACCTCA
  TCAACTACGGCACCAAGGGGAAGGTGCGCCTCCCGGCCCTGCACATCGCGGCCCGCAACGACGACACGCG
  CACGGCTGCGGTGCTGCTGCAGAACGACCCCAACCCGGACGTGCTTTCCAAGACGGGATTCACGCCCCTG
  CACATTGCGGCTCACTACGAGAACCTCAACGTGGCCCAGTTGCTCCTCAACAGAGGAGCCAGCGTCAATT
  TCACACCACAGAACGGCATCACGCCACTGCACATCGCCTCCCGCAGGGGCAACGTGATCATGGTGCGGCT
  GCTGCTGGATCGGGGAGCCCAGATAGAAACCAAGACCAAGGACGAATTGACACCTCTCCACTGTGCAGCT
  CGAAATGGGCACGTGCGAATCTCAGAGATCCTGCTGGACCACGGGGCACCAATCCAAGCCAAAACCAAGA
  ACGGCCTGTCCCCAATTCACATGGCGGCTCAGGGAGACCACCTCGACTGTGTCCGGCTCCTGTTGCAATA
  CGACGCAGAGATAGACGACATCACCCTGGACCACCTGACCCCACTCCACGTGGCTGCCCACTGTGGACAC
  CACAGGGTGGCTAAGGTCCTTCTGGATAAAGGGGCCAAACCCAACTCCAGAGCCCTGAATGGCTTTACCC
  CCTTACACATCGCCTGCAAAAAGAACCACGTCCGTGTCATGGAGCTGCTGCTGAAGACGGGAGCCTCGAT
  CGACGCGGTCACCGAGTCTGGCCTGACACCTCTCCACGTGGCCTCCTTCATGGGGCACCTTCCCATCGTG
  AAGAACCTCCTGCAGCGGGGGGCGTCGCCCAACGTCTCCAACGTGAAAGTGGAGACCCCGCTACACATGG
  CAGCCAGAGCCGGGCACACGGAAGTGGCCAAATATTTACTCCAGAACAAAGCCAAAGTCAATGCCAAGGC
  CAAGGATGACCAGACCCCACTTCACTGTGCAGCTCGCATCGGCCACACAAACATGGTGAAGCTCCTGCTG
  GAAAATAACGCCAACCCCAACCTGGCCACCACCGCCGGGCACACCCCCCTGCACATTGCAGCCCGTGAGG
  GCCATGTGGAAACAGTCCTGGCCCTTCTGGAAAAGGAAGCATCCCAGGCCTGCATGACCAAGAAAGGATT
  TACCCCTCTGCACGTGGCGGCCAAGTACGGGAAGGTGCGGGTGGCAGAGCTGCTGCTGGAGCGGGACGCA
  CACCCGAATGCTGCCGGAAAAAATGGCCTGACCCCCCTGCACGTGGCCGTCCATCACAACAACCTGGACA
  TCGTCAAGCTGCTGCTTCCCCGGGGCGGCTCCCCGCACAGCCCTGCCTGGAATGGCTACACCCCTTTGCA
  CATCGCTGCCAAGCAGAACCAGGTGGAGGTGGCCCGTAGTCTGCTGCAGTATGGGGGCTCAGCAAACGCC
  GAGTCGGTGCAAGGTGTGACGCCCCTTCACCTGGCCGCCCAGGAGGGCCACGCAGAGATGGTGGCTCTGC
  TGCTCTCGAAACAAGCCAATGGCAACCTGGGGAACAAGAGCGGACTCACTCCCCTCCATCTGGTAGCACA
  AGAAGGCCACGTTCCAGTGGCAGATGTGCTGATCAAACACGGCGTCATGGTGGACGCCACCACCCGGATG
  GGCTACACTCCCCTCCATGTGGCCAGTCACTATGGAAACATCAAGCTGGTGAAGTTTCTGCTGCAGCACC
  AGGCAGATGTCAATGCCAAGACCAAGCTAGGATACAGCCCCCTGCACCAGGCAGCCCAGCAGGGACACAC
  AGACATCGTGACTCTGCTTCTGAAAAACGGTGCTTCCCCAAACGAGGTCAGCTCGGATGGAACCACACCT
  CTGGCCATAGCCAAGCGCTTGGGCTACATTTCTGTCACCGACGTGCTCAAGGTCGTCACGGATGAAACCA
  GTTTCGTGTTAGTCAGTGATAAGCATCGAATGAGTTTCCCTGAGACAGTTGATGAGATCCTGGATGTCTC
  GGAAGATGAAGGGGAAGAACTCATCAGCTTCAAGGCTGAGAGGCGGGATTCCAGGGATGTTGATGAAGAG
  AAGGAGCTGCTGGATTTTGTGCCGAAGCTAGACCAAGTGGTGGAATCTCCAGCCATCCCCAGGATTCCCT
  GTGCCATGCCTGAGACAGTGGTGATCAGGTCAGAAGAGCAGGAGCAGGCATCTAAAGAGTATGATGAGGA
  CTCCCTCATCCCCAGCAGCCCGGCCACCGAGACCTCAGACAACATCAGCCCGGTGGCCAGCCCGGTGCAT
  ACAGGGTTTCTGGTGAGCTTCATGGTTGACGCCCGGGGTGGTTCCATGAGAGGAAGTCGCCACAACGGCC
  TGCGAGTGGTGATCCCGCCACGGACGTGCGCAGCGCCCACCCGCATCACCTGCCGCCTGGTCAAGCCCCA
  GAAGCTCAGCACGCCGCCCCCACTGGCCGAGGAGGAGGGCCTGGCCAGCAGGATCATAGCACTGGGGCCC
  ACGGGGGCACAGTTCCTGAGCCCTGTAATCGTGGAGATCCCGCACTTTGCCTCCCATGGCCGTGGAGACC
  GCGAGCTCGTGGTTCTGAGGAGCGAAAACGGCTCCGTGTGGAAGGAGCACAGGAGCCGCTATGGAGAGAG
  CTACCTGGATCAGATCCTCAACGGGATGGACGAAGAGCTGGGGAGCCTGGAGGAGCTAGAGAAGAAGAGG
  GTGTGCCGAATCATCACCACCGACTTCCCGCTGTACTTCGTGATCATGTCACGGCTCTGCCAGGACTACG
  ACACCATCGGTCCCGAAGGGGGCTCCCTGAAGAGCAAGCTGGTGCCCCTGGTACAGGCAACGTTCCCGGA
  GAATGCCGTCACCAAGAGAGTGAAGCTGGCTCTGCAGGCCCAGCCTGTCCCGGATGAGCTTGTCACTAAG
  CTCCTGGGCAACCAGGCCACATTCAGCCCCATTGTCACCGTGGAGCCCCGGCGCCGGAAGTTCCACCGCC
  CCATTGGGCTTCGGATCCCACTACCTCCTTCCTGGACCGACAACCCGAGGGACAGCGGGGAGGGAGACAC
  CACCAGCCTGCGCCTGCTTTGCAGCGTCATTGGAGGAACAGACCAAGCCCAGTGGGAAGACATAACAGGA
  ACCACCAAACTTGTATATGCCAACGAGTGCGCCAACTTCACCACCAATGTCTCTGCCAGGTTTTGGCTGT
  CGGACTGTCCTCGGACTGCTGAGGCTGTGAACTTTGCCACCCTGCTGTACAAAGAGCTCACTGCAGTGCC
  CTACATGGCCAAATTCGTCATCTTTGCCAAGATGAATGACCCCCGAGAGGGGCGCCTGCGCTGCTACTGC
  ATGACAGATGATAAAGTGGACAAGACCCTGGAGCAGCATGAGAACTTCGTGGAGGTGGCCCGGAGCAGGG
  ACATAGAGGTGTTGGAAGGAATGTCCCTGTTTGCAGAACTCTCTGGGAACCTGGTGCCTGTGAAGAAAGC
  TGCCCAGCAGCGGAGCTTCCACTTCCAGTCATTTCGGGAGAACCGTCTGGCCATGCCTGTAAAGGTGAGG
  GACAGCAGTCGAGAGCCGGGAGGGTCCCTGTCGTTTCTGCGCAAGGCGATGAAGTACGAGGACACCCAGC
  ACATTCTCTGCCACCTGAACATCACCATGCCCCCCTGCGCCAAGGGAAGTGGAGCCGAAGATAGGAGAAG
  GACCCCGACGCCCCTGGCCCTGCGATACAGCATTCTCAGTGAGTCCACACCAGGTTCTCTCAGTGGGACA
  GAGCAGGCAGAGATGAAGATGGCTGTTATCTCAGAGCACCTCGGTCTCAGCTGGGCAGAGTTGGCCCGGG
  AGCTGCAGTTCAGTGTGGAAGACATCAACAGGATCCGAGTGGAAAATCCCAACTCCCTGTTGGAGCAGAG
  TGTGGCCTTGCTGAACCTCTGGGTCATCCGTGAAGGCCAAAACGCAAACATGGAGAATCTGTACACAGCC
  CTGCAGAGCATTGACCGTGGCGAGATCGTGAACATGCTGGAGGGTTCCGGCCGACAGAGCCGCAACTTGA
  AGCCAGACAGGCGGCACACCGACCGCGACTACTCGCTGTCACCCTCCCAGATGAATGGTTACTCCTCACT
  GCAGGACGAGCTGCTGTCCCCTGCCTCCCTGGGCTGTGCACTTTCCTCTCCGCTACGTGCAGACCAGTAC
  TGGAATGAGGTGGCCGTCCTAGACGCCATCCCCTTGGCGGCCACGGAGCATGACACCATGCTGGAGATGT
  CTGACATGCAGGTGTGGTCTGCGGGCCTCACGCCTTCTCTGGTCACTGCTGAGGACTCCTCTCTGGAGTG
  TAGCAAGGCTGAGGACTCTGATGCCACAGGTCACGAGTGGAAGTTGGAGGGGGCACTCTCAGAGGAACCG
  CGGGGCCCCGAGTTGGGCTCTCTGGAACTTGTGGAGGACGACACAGTGGATTCAGATGCCACAAATGGCC
  TTATCGATTTGCTTGAACAGGAGGAAGGTCAGAGGTCAGAAGAGAAGCTGCCAGGTTCTAAGAGGCAGGA
  TGACGCGACAGGTGCAGGGCAGGACTCAGAGAATGAAGTGTCTCTTGTTTCAGGCCATCAGAGGGGGCAA
  GCCCGAATCACACATTCCCCCACCGTGAGTCAGGTGACGGAGAGGAGTCAGGACAGACTGCAGGACTGGG
  ATGCAGACGGCTCGATTGTCTCATACCTGCAAGATGCTGCACAAGGTTCCTGGCAAGAGGAGGTCACGCA
  AGGTCCACACTCATTCCAGGGAACAAGTACCATGACTGAAGGGCTAGAGCCCGGTGGATCTCAGGAGTAC
  GAGAAGGTCCTGGTGTCTGTAAGTGAGCACACGTGGACAGAACAGCCCGAGGCTGAGAGCTCCCAGGCCG
  ACAGGGACCGGAGGCAGCAAGGCCAAGAAGAGCAGGTGCAGGAGGCCAAGAACACCTTCACCCAAGTGGT
  GCAGGGGAATGAGTTTCAGAATATTCCAGGGGAGCAGGTGACAGAGGAGCAATTCACGGATGAGCAGGGC
  AACATTGTCACCAAGAAGATCATTCGCAAGGTGGTTCGACAGATAGACTTGTCCAGCGCCGATGCCGCCC
  AGGAGCACGAGGAGGTGACTGTAGAGGGGCCCCTGGAGGATCCCAGTGAGCTGGAGGTCGATATTGATTA
  CTTTATGAAACACTCCAAGGATCACACCTCGACACCCAACCCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC220763 representing NM_020476
  Red=Cloning site Green=Tags(s)
  
  MPYSVGFREADAATSFLRAARSGNLDKALDHLRNGVDINTCNQNGLNGLHLASKEGHVKMVVELLHKEII
  LETTTKKGNTALHIAALAGQDEVVRELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLENGANQNVA
  TEDGFTPLAVALQQGHENVVAHLINYGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPL
  HIAAHYENLNVAQLLLNRGASVNFTPQNGITPLHIASRRGNVIMVRLLLDRGAQIETKTKDELTPLHCAA
  RNGHVRISEILLDHGAPIQAKTKNGLSPIHMAAQGDHLDCVRLLLQYDAEIDDITLDHLTPLHVAAHCGH
  HRVAKVLLDKGAKPNSRALNGFTPLHIACKKNHVRVMELLLKTGASIDAVTESGLTPLHVASFMGHLPIV
  KNLLQRGASPNVSNVKVETPLHMAARAGHTEVAKYLLQNKAKVNAKAKDDQTPLHCAARIGHTNMVKLLL
  ENNANPNLATTAGHTPLHIAAREGHVETVLALLEKEASQACMTKKGFTPLHVAAKYGKVRVAELLLERDA
  HPNAAGKNGLTPLHVAVHHNNLDIVKLLLPRGGSPHSPAWNGYTPLHIAAKQNQVEVARSLLQYGGSANA
  ESVQGVTPLHLAAQEGHAEMVALLLSKQANGNLGNKSGLTPLHLVAQEGHVPVADVLIKHGVMVDATTRM
  GYTPLHVASHYGNIKLVKFLLQHQADVNAKTKLGYSPLHQAAQQGHTDIVTLLLKNGASPNEVSSDGTTP
  LAIAKRLGYISVTDVLKVVTDETSFVLVSDKHRMSFPETVDEILDVSEDEGEELISFKAERRDSRDVDEE
  KELLDFVPKLDQVVESPAIPRIPCAMPETVVIRSEEQEQASKEYDEDSLIPSSPATETSDNISPVASPVH
  TGFLVSFMVDARGGSMRGSRHNGLRVVIPPRTCAAPTRITCRLVKPQKLSTPPPLAEEEGLASRIIALGP
  TGAQFLSPVIVEIPHFASHGRGDRELVVLRSENGSVWKEHRSRYGESYLDQILNGMDEELGSLEELEKKR
  VCRIITTDFPLYFVIMSRLCQDYDTIGPEGGSLKSKLVPLVQATFPENAVTKRVKLALQAQPVPDELVTK
  LLGNQATFSPIVTVEPRRRKFHRPIGLRIPLPPSWTDNPRDSGEGDTTSLRLLCSVIGGTDQAQWEDITG
  TTKLVYANECANFTTNVSARFWLSDCPRTAEAVNFATLLYKELTAVPYMAKFVIFAKMNDPREGRLRCYC
  MTDDKVDKTLEQHENFVEVARSRDIEVLEGMSLFAELSGNLVPVKKAAQQRSFHFQSFRENRLAMPVKVR
  DSSREPGGSLSFLRKAMKYEDTQHILCHLNITMPPCAKGSGAEDRRRTPTPLALRYSILSESTPGSLSGT
  EQAEMKMAVISEHLGLSWAELARELQFSVEDINRIRVENPNSLLEQSVALLNLWVIREGQNANMENLYTA
  LQSIDRGEIVNMLEGSGRQSRNLKPDRRHTDRDYSLSPSQMNGYSSLQDELLSPASLGCALSSPLRADQY
  WNEVAVLDAIPLAATEHDTMLEMSDMQVWSAGLTPSLVTAEDSSLECSKAEDSDATGHEWKLEGALSEEP
  RGPELGSLELVEDDTVDSDATNGLIDLLEQEEGQRSEEKLPGSKRQDDATGAGQDSENEVSLVSGHQRGQ
  ARITHSPTVSQVTERSQDRLQDWDADGSIVSYLQDAAQGSWQEEVTQGPHSFQGTSTMTEGLEPGGSQEY
  EKVLVSVSEHTWTEQPEAESSQADRDRRQQGQEEQVQEAKNTFTQVVQGNEFQNIPGEQVTEEQFTDEQG
  NIVTKKIIRKVVRQIDLSSADAAQEHEEVTVEGPLEDPSELEVDIDYFMKHSKDHTSTPNP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020476
• ORF Size: 5643 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020476.3
• RefSeq Size: 8240 bp
• RefSeq ORF: 5646 bp
• Locus ID: 286
• UniProt ID: P16157
• Cytogenetics: 8p11.21
• Protein Families: Transmembrane
• MW: 206.1 kDa
• Gene Summary: Ankyrins are a family of proteins that link the integral membrane proteins to the underlying spectrin-actin cytoskeleton and play key roles in activities such as cell motility, activation, proliferation, contact and the maintenance of specialized membrane domains. Multiple isoforms of ankyrin with different affinities for various target proteins are expressed in a tissue-specific, developmentally regulated manner. Most ankyrins are typically composed of three structural domains: an amino-terminal domain containing multiple ankyrin repeats; a central region with a highly conserved spectrin binding domain; and a carboxy-terminal regulatory domain which is the least conserved and subject to variation. Ankyrin 1, the prototype of this family, was first discovered in the erythrocytes, but since has also been found in brain and muscles. Mutations in erythrocytic ankyrin 1 have been associated in approximately half of all patients with hereditary spherocytosis. Complex patterns of alternative splicing in the regulatory domain, giving rise to different isoforms of ankyrin 1 have been described. Truncated muscle-specific isoforms of ankyrin 1 resulting from usage of an alternate promoter have also been identified. [provided by RefSeq, Dec 2008]