CDCP1 (NM_022842) Human Tagged ORF Clone

CAT#: RC220633

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CDCP1 (Myc-DDK-tagged)-Human CUB domain containing protein 1 (CDCP1), transcript variant 1



  "NM_022842" in other vectors (6)

Reconstitution Protocol

USD 765.00

In Stock*

Size
    • 10 ug

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Frequently bought together (4)
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Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
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Other products for "CDCP1"

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol CDCP1
Synonyms CD318; SIMA135; TRASK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC220633 representing NM_022842
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCGGCCTGAACTGCGGGGTCTCTATCGCACTGCTAGGGGTTCTGCTGCTGGGTGCGGCGCGCCTGC
CGCGCGGGGCAGAAGCTTTTGAGATTGCTCTGCCACGAGAAAGCAACATTACAGTTCTCATAAAGCTGGG
GACCCCGACTCTGCTGGCAAAACCCTGTTACATCGTCATTTCTAAAAGACATATAACCATGTTGTCCATC
AAGTCTGGAGAAAGAATAGTCTTTACCTTTAGCTGCCAGAGTCCTGAGAATCACTTTGTCATAGAGATCC
AGAAAAATATTGACTGTATGTCAGGCCCATGTCCTTTTGGGGAGGTTCAGCTTCAGCCCTCGACATCGTT
GTTGCCTACCCTCAACAGAACTTTCATCTGGGATGTCAAAGCTCATAAGAGCATCGGTTTAGAGCTGCAG
TTTTCCATCCCTCGCCTGAGGCAGATCGGTCCGGGTGAGAGCTGCCCAGACGGAGTCACTCACTCCATCA
GCGGCCGAATCGATGCCACCGTGGTCAGGATCGGAACCTTCTGCAGCAATGGCACTGTGTCCCGGATCAA
GATGCAAGAAGGAGTGAAAATGGCCTTACACCTCCCATGGTTCCACCCCAGAAATGTCTCCGGCTTCAGC
ATTGCAAACCGCTCATCTATAAAACGTCTGTGCATCATCGAGTCTGTGTTTGAGGGTGAAGGCTCAGCAA
CCCTGATGTCTGCCAACTACCCAGAAGGCTTCCCTGAGGATGAGCTCATGACGTGGCAGTTTGTCGTTCC
TGCACACCTGCGGGCCAGCGTCTCCTTCCTCAACTTCAACCTCTCCAACTGTGAGAGGAAGGAGGAGCGG
GTTGAATACTACATCCCGGGCTCCACCACCAACCCCGAGGTGTTCAAGCTGGAGGACAAGCAGCCTGGGA
ACATGGCGGGGAACTTCAACCTCTCTCTGCAAGGCTGTGACCAAGATGCCCAAAGTCCAGGGATCCTCCG
GCTGCAGTTCCAAGTTTTGGTCCAACATCCACAAAATGAAAGCAATAAAATCTACGTGGTTGACTTGAGT
AATGAGCGAGCCATGTCACTCACCATCGAGCCACGGCCCGTCAAACAGAGCCGCAAGTTTGTCCCTGGCT
GTTTCGTGTGTCTAGAATCTCGGACCTGCAGTAGCAACCTCACCCTGACATCTGGCTCCAAACACAAAAT
CTCCTTCCTTTGTGATGATCTGACACGTCTGTGGATGAATGTGGAAAAAACCATAAGCTGCACAGACCAC
CGGTACTGCCAAAGGAAATCCTACTCACTCCAGGTGCCCAGTGACATCCTCCACCTGCCTGTGGAGCTGC
ATGACTTCTCCTGGAAGCTGCTGGTGCCCAAGGACAGGCTCAGCCTGGTGCTGGTGCCAGCCCAGAAGCT
GCAGCAGCATACACACGAGAAGCCCTGCAACACCAGCTTCAGCTACCTCGTGGCCAGTGCCATACCCAGC
CAGGACCTGTACTTCGGCTCCTTCTGCCCGGGAGGCTCTATCAAGCAGATCCAGGTGAAGCAGAACATCT
CGGTGACCCTTCGCACCTTTGCCCCCAGCTTCCGACAAGAGGCCTCCAGGCAGGGTCTGACGGTGTCCTT
TATACCTTATTTCAAAGAGGAAGGCGTTTTCACGGTGACCCCTGACACAAAAAGCAAGGTCTACCTGAGG
ACCCCCAACTGGGACCGGGGCCTGCCATCCCTCACCTCTGTGTCCTGGAACATCAGCGTGCCCAGAGACC
AGGTGGCCTGCCTGACTTTCTTTAAGGAGCGGAGCGGCGTGGTCTGCCAGACAGGGCGCGCATTCATGAT
CATCCAGGAGCAGCGGACCCGGGCTGAGGAGATCTTCAGCCTGGACGAGGATGTGCTCCCCAAGCCAAGC
TTCCACCATCACAGCTTCTGGGTCAACATCTCTAACTGCAGCCCCACGAGCGGCAAGCAGCTAGACCTGC
TCTTCTCGGTGACACTTACCCCAAGGACTGTGGACTTGACTGTCATCCTCATCGCAGCGGTGGGAGGTGG
AGTCTTACTGCTGTCTGCCCTCGGGCTCATCATTTGCTGTGTGAAAAAGAAGAAAAAGAAGACAAACAAG
GGCCCCGCTGTGGGTATCTACAATGGCAACATCAATACTGAGATGCCGAGGCAGCCAAAAAAGTTTCAGA
AAGGGCGAAAGGACAATGACTCCCATGTGTATGCAGTCATCGAGGACACCATGGTATATGGGCATCTGCT
ACAGGATTCCAGCGGCTCCTTCCTGCAGCCAGAGGTGGACACCTACCGGCCGTTCCAGGGCACCATGGGG
GTCTGTCCTCCCTCCCCACCCACCATATGCTCCAGGGCCCCAACTGCAAAGTTGGCCACTGAGGAGCCAC
CTCCTCGCTCCCCTCCTGAGTCTGAGAGTGAACCGTACACCTTCTCCCATCCCAACAATGGGGATGTAAG
CAGCAAGGACACAGACATTCCCTTACTGAACACTCAGGAGCCCATGGAGCCAGCAGAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC220633 representing NM_022842
Red=Cloning site Green=Tags(s)

MAGLNCGVSIALLGVLLLGAARLPRGAEAFEIALPRESNITVLIKLGTPTLLAKPCYIVISKRHITMLSI
KSGERIVFTFSCQSPENHFVIEIQKNIDCMSGPCPFGEVQLQPSTSLLPTLNRTFIWDVKAHKSIGLELQ
FSIPRLRQIGPGESCPDGVTHSISGRIDATVVRIGTFCSNGTVSRIKMQEGVKMALHLPWFHPRNVSGFS
IANRSSIKRLCIIESVFEGEGSATLMSANYPEGFPEDELMTWQFVVPAHLRASVSFLNFNLSNCERKEER
VEYYIPGSTTNPEVFKLEDKQPGNMAGNFNLSLQGCDQDAQSPGILRLQFQVLVQHPQNESNKIYVVDLS
NERAMSLTIEPRPVKQSRKFVPGCFVCLESRTCSSNLTLTSGSKHKISFLCDDLTRLWMNVEKTISCTDH
RYCQRKSYSLQVPSDILHLPVELHDFSWKLLVPKDRLSLVLVPAQKLQQHTHEKPCNTSFSYLVASAIPS
QDLYFGSFCPGGSIKQIQVKQNISVTLRTFAPSFRQEASRQGLTVSFIPYFKEEGVFTVTPDTKSKVYLR
TPNWDRGLPSLTSVSWNISVPRDQVACLTFFKERSGVVCQTGRAFMIIQEQRTRAEEIFSLDEDVLPKPS
FHHHSFWVNISNCSPTSGKQLDLLFSVTLTPRTVDLTVILIAAVGGGVLLLSALGLIICCVKKKKKKTNK
GPAVGIYNGNINTEMPRQPKKFQKGRKDNDSHVYAVIEDTMVYGHLLQDSSGSFLQPEVDTYRPFQGTMG
VCPPSPPTICSRAPTAKLATEEPPPRSPPESESEPYTFSHPNNGDVSSKDTDIPLLNTQEPMEPAE

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_022842
ORF Size 2508 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_022842.5
RefSeq Size 6017 bp
RefSeq ORF 2511 bp
Locus ID 64866
UniProt ID Q9H5V8
Cytogenetics 3p21.31
Protein Families ES Cell Differentiation/IPS, Transmembrane
MW 92.8 kDa
Gene Summary This gene encodes a transmembrane protein which contains three extracellular CUB domains and acts as a substrate for Src family kinases. The protein plays a role in the tyrosine phosphorylation-dependent regulation of cellular events that are involved in tumor invasion and metastasis. Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, May 2013]

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