RIT1 (NM_006912) Human Tagged ORF Clone

CAT#: RC220552

RIT1 (Myc-DDK-tagged)-Human Ras-like without CAAX 1 (RIT1)

Product Images

Western blot validation of overexpression lysate (Cat# LY416316) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC220552 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified RIT1 protein (Cat# TP320552). The protein was produced from HEK293T cells transfected with RIT1 cDNA clone (Cat# RC220552) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RIT1
• Synonyms: NS8; RIBB; RIT; ROC1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC220552 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATTCTGGAACTCGCCCAGTTGGTAGCTGCTGTAGCAGCCCCGCTGGGCTCTCACGGGAGTACAAAC
  TAGTGATGCTGGGTGCTGGTGGTGTAGGGAAGAGTGCCATGACCATGCAGTTCATCAGCCACCGATTCCC
  AGAAGATCATGATCCCACCATTGAAGATGCTTATAAGATCAGGATCCGTATTGATGATGAGCCTGCCAAT
  CTGGACATTTTGGATACAGCTGGACAGGCAGAGTTTACAGCCATGCGGGACCAGTATATGAGGGCAGGAG
  AAGGGTTTATCATCTGTTACTCTATCACGGATCGTCGAAGTTTCCATGAAGTTCGTGAGTTTAAACAGCT
  TATTTATCGAGTCCGACGTACTGACGATACACCTGTGGTTCTTGTGGGAAACAAGTCAGACCTCAAACAG
  CTAAGACAGGTCACCAAGGAAGAAGGATTGGCCTTGGCCCGAGAATTCAGCTGTCCCTTTTTTGAGACAT
  CTGCTGCATACCGCTACTATATTGATGATGTTTTCCATGCCCTTGTACGGGAGATACGTAGGAAAGAAAA
  GGAGGCAGTACTGGCCATGGAGAAAAAATCTAAGCCCAAAAACAGTGTATGGAAGAGGCTAAAATCACCA
  TTCCGGAAGAAGAAAGATTCAGTAACT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC220552 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MDSGTRPVGSCCSSPAGLSREYKLVMLGAGGVGKSAMTMQFISHRFPEDHDPTIEDAYKIRIRIDDEPAN
  LDILDTAGQAEFTAMRDQYMRAGEGFIICYSITDRRSFHEVREFKQLIYRVRRTDDTPVVLVGNKSDLKQ
  LRQVTKEEGLALAREFSCPFFETSAAYRYYIDDVFHALVREIRRKEKEAVLAMEKKSKPKNSVWKRLKSP
  FRKKKDSVT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006912
• ORF Size: 657 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006912.6
• RefSeq Size: 3455 bp
• RefSeq ORF: 660 bp
• Locus ID: 6016
• UniProt ID: Q92963
• Cytogenetics: 1q22
• Domains: ras, RAN, RAS, RHO, RAB
• MW: 25.1 kDa
• Gene Summary: This gene encodes a member of a subfamily of Ras-related GTPases. The encoded protein is involved in regulating p38 MAPK-dependent signaling cascades related to cellular stress. This protein also cooperates with nerve growth factor to promote neuronal development and regeneration. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Feb 2012]