Natriuretic Peptide Receptor B (NPR2) (NM_003995) Human Tagged ORF Clone

CAT#: RC220459

NPR2 (Myc-DDK-tagged)-Human natriuretic peptide receptor B/guanylate cyclase B (atrionatriuretic peptide receptor B) (NPR2)

Product Images

Circular map for RC220459

• 

  Western blot validation of overexpression lysate (Cat# LY418262) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC220459 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Natriuretic Peptide Receptor B
• Synonyms: AMDM; ANPb; ANPRB; ECDM; GC-B; GCB; GUC2B; GUCY2B; NPRB; NPRBi; SNSK
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC220459 representing NM_003995.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGCGCTGCCATCACTTCTGCTGTTGGTGGCAGCCCTGGCAGGTGGGGTGCGTCCTCCCGGGGCGCGG
  AACCTGACGCTGGCGGTGGTGCTGCCAGAACACAACCTGAGCTATGCCTGGGCCTGGCCACGGGTGGGA
  CCCGCTGTGGCACTAGCTGTGGAGGCTCTGGGCCGGGCACTGCCCGTGGACCTGCGGTTTGTCAGCTCC
  GAACTGGAAGGCGCCTGCTCTGAGTACCTGGCACCGCTGAGCGCTGTGGACCTCAAGCTGTACCATGAC
  CCCGACCTGCTGTTAGGTCCCGGTTGCGTGTACCCTGCTGCCTCTGTGGCCCGCTTTGCCTCCCACTGG
  CGCCTTCCCCTGCTGACTGCGGGTGCTGTGGCCTCTGGTTTTTCGGCTAAGAATGACCATTATCGTACC
  CTGGTTCGCACTGGCCCCTCTGCTCCCAAGCTGGGTGAGTTTGTGGTGACACTACACGGGCACTTCAAT
  TGGACTGCCCGTGCTGCCTTGCTGTACCTGGATGCTCGCACAGATGACCGGCCTCACTACTTCACCATC
  GAGGGCGTCTTTGAGGCCCTGCAGGGCAGCAACCTCAGTGTGCAGCACCAGGTGTATGCCCGAGAGCCA
  GGGGGCCCCGAGCAGGCCACCCACTTCATCCGGGCCAACGGGCGCATTGTGTATATCTGCGGCCCTCTG
  GAGATGCTGCATGAGATCCTGCTTCAGGCCCAGAGGGAGAATCTGACCAATGGGGATTATGTCTTCTTT
  TACCTGGATGTCTTTGGGGAGAGTCTCCGTGCAGGCCCCACACGTGCTACAGGCCGGCCCTGGCAGGAC
  AATCGCACCCGGGAACAGGCCCAGGCCCTCAGAGAGGCCTTTCAGACTGTATTGGTGATCACGTACCGA
  GAACCCCCAAATCCTGAGTATCAGGAATTCCAGAATCGTCTGCTGATAAGAGCCCGGGAAGACTTTGGT
  GTGGAGCTGGGCCCTTCCCTGATGAACCTCATCGCTGGCTGCTTCTATGATGGGATCCTGCTATATGCT
  GAAGTCCTGAATGAGACAATACAGGAAGGAGGCACCCGGGAGGATGGACTTCGAATTGTGGAAAAGATG
  CAGGGACGAAGATATCACGGTGTAACTGGGCTGGTTGTCATGGACAAGAACAATGACCGAGAGACTGAC
  TTTGTCCTCTGGGCCATGGGAGACCTGGATTCTGGGGACTTTCAGCCTGCAGCCCACTACTCGGGAGCT
  GAGAAGCAGATTTGGTGGACGGGACGGCCTATTCCCTGGGTGAAGGGGGCTCCTCCCTCGGACAATCCC
  CCCTGTGCCTTTGACTTGGACGACCCATCCTGTGATAAAACTCCACTTTCAACCCTGGCAATTGTGGCT
  CTGGGCACAGGAATCACCTTCATCATGTTTGGTGTTTCCAGCTTCCTAATTTTCCGAAAGCTGATGCTG
  GAGAAGGAGCTGGCTAGCATGTTGTGGCGTATTCGCTGGGAAGAACTGCAGTTTGGCAACTCAGAGCGT
  TATCACAAAGGTGCAGGCAGTCGCCTCACACTGTCGCTGCGGGGATCCAGTTACGGCTCGCTCATGACA
  GCCCATGGGAAATACCAGATCTTTGCCAACACCGGTCACTTCAAGGGAAATGTTGTCGCCATCAAACAT
  GTGAATAAGAAGCGCATTGAGCTGACCCGGCAGGTTCTGTTTGAACTCAAACATATGAGAGATGTTCAG
  TTCAACCATCTCACTCGCTTCATTGGCGCCTGCATAGACCCTCCCAACATTTGCATTGTCACTGAATAT
  TGTCCTCGTGGGAGTTTACAGGATATTCTAGAAAATGACAGCATCAACTTGGACTGGATGTTTCGTTAT
  TCACTCATTAATGACCTTGTTAAGGGCATGGCCTTTCTCCACAACAGCATTATTTCATCGCATGGGAGT
  CTCAAGTCCTCCAACTGTGTGGTGGATAGTCGTTTTGTGCTCAAAATCACAGACTATGGCCTGGCCAGC
  TTCCGATCAACTGCTGAACCTGATGACAGCCATGCCCTCTATGCCAAGAAGCTGTGGACTGCCCCAGAA
  CTGCTCAGTGGGAACCCCTTGCCAACCACAGGCATGCAGAAGGCTGACGTCTATAGCTTTGGGATCATC
  CTGCAGGAGATAGCACTTCGCAGTGGTCCTTTCTACTTGGAGGGCCTGGACCTCAGCCCCAAAGAGATT
  GTCCAGAAGGTACGAAATGGTCAGCGGCCATATTTCCGGCCAAGCATTGACCGGACCCAACTGAATGAA
  GAGCTAGTTTTGCTGATGGAGCGATGTTGGGCTCAGGACCCAGCTGAGCGGCCAGACTTTGGACAGATT
  AAGGGCTTCATTCGGCGCTTTAACAAGGAGGGTGGCACCAGCATATTGGACAACCTCCTGCTGCGCATG
  GAACAGTATGCCAATAACTTGGAGAAGCTGGTGGAGGAACGCACACAGGCCTATCTGGAGGAAAAACGC
  AAGGCTGAAGCTCTGCTCTACCAAATCCTACCCCATTCAGTGGCAGAGCAGTTAAAACGGGGAGAGACT
  GTACAGGCTGAGGCCTTTGACAGTGTTACCATCTACTTCAGTGACATTGTTGGCTTCACAGCATTGTCA
  GCAGAGAGCACCCCCATGCAGGTAGTGACACTTCTTAATGACCTGTATACCTGCTTTGATGCCATAATT
  GACAACTTTGATGTCTACAAGGTGGAGACGATTGGGGATGCTTACATGGTGGTATCTGGCCTCCCAGGC
  CGAAATGGTCAACGCCATGCACCAGAAATTGCTCGTATGGCCCTAGCATTACTAGATGCAGTTTCTTCC
  TTTCGCATCCGCCACCGACCCCATGACCAGCTGAGGCTACGCATAGGGGTCCATACTGGGCCAGTCTGT
  GCTGGGGTTGTTGGCCTGAAGATGCCCCGTTATTGTCTTTTTGGAGACACAGTGAACACTGCTTCTCGA
  ATGGAGTCTAATGGTCAAGCGCTGAAGATCCATGTCTCCTCTACCACCAAGGATGCCCTAGATGAGCTA
  GGATGCTTCCAGCTAGAGCTTCGGGGGGATGTGGAAATGAAGGGAAAAGGAAAGATGCGAACATACTGG
  CTCTTAGGAGAGCGGAAAGGACCTCCTGGACTCCTG
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
  Protein Sequence

  >Peptide sequence encoded by RC220459
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MALPSLLLLVAALAGGVRPPGARNLTLAVVLPEHNLSYAWAWPRVGPAVALAVEALGRALPVDLRFVSS
  ELEGACSEYLAPLSAVDLKLYHDPDLLLGPGCVYPAASVARFASHWRLPLLTAGAVASGFSAKNDHYRT
  LVRTGPSAPKLGEFVVTLHGHFNWTARAALLYLDARTDDRPHYFTIEGVFEALQGSNLSVQHQVYAREP
  GGPEQATHFIRANGRIVYICGPLEMLHEILLQAQRENLTNGDYVFFYLDVFGESLRAGPTRATGRPWQD
  NRTREQAQALREAFQTVLVITYREPPNPEYQEFQNRLLIRAREDFGVELGPSLMNLIAGCFYDGILLYA
  EVLNETIQEGGTREDGLRIVEKMQGRRYHGVTGLVVMDKNNDRETDFVLWAMGDLDSGDFQPAAHYSGA
  EKQIWWTGRPIPWVKGAPPSDNPPCAFDLDDPSCDKTPLSTLAIVALGTGITFIMFGVSSFLIFRKLML
  EKELASMLWRIRWEELQFGNSERYHKGAGSRLTLSLRGSSYGSLMTAHGKYQIFANTGHFKGNVVAIKH
  VNKKRIELTRQVLFELKHMRDVQFNHLTRFIGACIDPPNICIVTEYCPRGSLQDILENDSINLDWMFRY
  SLINDLVKGMAFLHNSIISSHGSLKSSNCVVDSRFVLKITDYGLASFRSTAEPDDSHALYAKKLWTAPE
  LLSGNPLPTTGMQKADVYSFGIILQEIALRSGPFYLEGLDLSPKEIVQKVRNGQRPYFRPSIDRTQLNE
  ELVLLMERCWAQDPAERPDFGQIKGFIRRFNKEGGTSILDNLLLRMEQYANNLEKLVEERTQAYLEEKR
  KAEALLYQILPHSVAEQLKRGETVQAEAFDSVTIYFSDIVGFTALSAESTPMQVVTLLNDLYTCFDAII
  DNFDVYKVETIGDAYMVVSGLPGRNGQRHAPEIARMALALLDAVSSFRIRHRPHDQLRLRIGVHTGPVC
  AGVVGLKMPRYCLFGDTVNTASRMESNGQALKIHVSSTTKDALDELGCFQLELRGDVEMKGKGKMRTYW
  LLGERKGPPGLL
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003995
• ORF Size: 3141 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.

Reference Data

• RefSeq Size: 3447 bp
• RefSeq ORF: 3144 bp
• Locus ID: 4882
• UniProt ID: P20594
• Cytogenetics: 9p13.3
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Purine metabolism, Vascular smooth muscle contraction
• MW: 117 kDa
• Gene Summary: This gene encodes natriuretic peptide receptor B, one of two integral membrane receptors for natriuretic peptides. Both NPR1 and NPR2 contain five functional domains: an extracellular ligand-binding domain, a single membrane-spanning region, and intracellularly a protein kinase homology domain, a helical hinge region involved in oligomerization, and a carboxyl-terminal guanylyl cyclase catalytic domain. The protein is the primary receptor for C-type natriuretic peptide (CNP), which upon ligand binding exhibits greatly increased guanylyl cyclase activity. Mutations in this gene are the cause of acromesomelic dysplasia Maroteaux type. [provided by RefSeq, Jul 2008]