ZIC3 (NM_003413) Human Tagged ORF Clone

CAT#: RC220375

ZIC3 (Myc-DDK-tagged)-Human Zic family member 3 (ZIC3)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY ZIC3 (Cat# RC220375, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ZIC3 (Cat# TA808167)(1:500). Positive lysates LY418705 (100ug) and LC418705 (20ug) can be purchased separately from OriGene.

• 

  Western blot validation of overexpression lysate (Cat# LY418705) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC220375 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ZIC3
• Synonyms: HTX; HTX1; VACTERLX; ZNF203
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC220375 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACGATGCTCCTGGACGGAGGCCCGCAGTTCCCTGGGCTGGGAGTGGGCAGCTTCGGCGCGCCGCGCC
  ACCACGAGATGCCCAACCGTGAGCCGGCAGGCATGGGGCTGAATCCCTTCGGGGACTCAACCCACGCCGC
  CGCCGCCGCCGCCGCCGCCGCTGCCTTCAAGCTGAGCCCTGCCGCGGCGCACGATCTATCTTCAGGCCAG
  AGCTCGGCTTTCACGCCGCAGGGTTCGGGCTACGCCAACGCCCTGGGCCACCATCACCACCACCATCACC
  ATCATCACCACACCAGCCAGGTGCCCAGCTACGGTGGCGCTGCCTCTGCCGCCTTCAACTCAACGCGCGA
  GTTTCTGTTCCGCCAGCGCAGCTCCGGGCTCAGTGAGGCGGCCTCGGGTGGCGGGCAGCACGGGCTCTTC
  GCCGGCTCGGCGAGCAGCCTGCATGCTCCAGCTGGCATCCCCGAGCCCCCTAGCTACTTGCTGTTTCCCG
  GGCTGCATGAGCAGGGCGCTGGGCACCCGTCGCCCACAGGGCACGTGGACAACAACCAGGTCCACCTGGG
  GCTGCGTGGGGAGCTGTTCGGCCGTGCTGACCCATACCGCCCAGTGGCCAGCCCGCGCACGGACCCCTAC
  GCGGCCGGCGCTCAGTTTCCTAACTACAGCCCCATGAACATGAACATGGGAGTGAACGTGGCGGCCCACC
  ACGGGCCCGGCGCCTTCTTCCGTTATATGCGGCAGCCTATCAAGCAGGAGCTGTCGTGCAAGTGGATCGA
  CGAGGCTCAGCTGAGCCGGCCCAAGAAGAGCTGCGACCGGACCTTCAGCACCATGCATGAGCTGGTGACA
  CATGTCACCATGGAGCATGTGGGGGGCCCGGAGCAGAACAACCACGTCTGCTACTGGGAGGAGTGCCCCC
  GGGAGGGCAAGTCTTTCAAGGCGAAGTACAAACTGGTCAACCACATCCGAGTGCACACGGGCGAGAAGCC
  CTTCCCATGCCCCTTCCCGGGCTGCGGGAAGATCTTTGCCCGTTCTGAGAACCTCAAGATCCACAAGAGG
  ACCCACACAGGTGAGAAACCTTTCAAATGTGAATTTGAAGGCTGTGACAGACGCTTTGCCAACAGCAGCG
  ACCGTAAGAAGCACATGCATGTGCATACCTCGGACAAGCCCTATATCTGCAAAGTGTGCGACAAGTCCTA
  CACGCACCCGAGCTCCCTGCGCAAACACATGAAGGTTCATGAATCTCAAGGGTCAGATTCCTCCCCTGCT
  GCCAGTTCAGGCTATGAATCTTCCACTCCACCCGCTATAGCTTCTGCAAACAGTAAAGATACCACTAAAA
  CCCCTTCTGCAGTTCAAACTAGCACCAGCCACAACCCTGGACTTCCTCCTAATTTTAACGAATGGTACGT
  C
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC220375 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MTMLLDGGPQFPGLGVGSFGAPRHHEMPNREPAGMGLNPFGDSTHAAAAAAAAAAFKLSPAAAHDLSSGQ
  SSAFTPQGSGYANALGHHHHHHHHHHHTSQVPSYGGAASAAFNSTREFLFRQRSSGLSEAASGGGQHGLF
  AGSASSLHAPAGIPEPPSYLLFPGLHEQGAGHPSPTGHVDNNQVHLGLRGELFGRADPYRPVASPRTDPY
  AAGAQFPNYSPMNMNMGVNVAAHHGPGAFFRYMRQPIKQELSCKWIDEAQLSRPKKSCDRTFSTMHELVT
  HVTMEHVGGPEQNNHVCYWEECPREGKSFKAKYKLVNHIRVHTGEKPFPCPFPGCGKIFARSENLKIHKR
  THTGEKPFKCEFEGCDRRFANSSDRKKHMHVHTSDKPYICKVCDKSYTHPSSLRKHMKVHESQGSDSSPA
  ASSGYESSTPPAIASANSKDTTKTPSAVQTSTSHNPGLPPNFNEWYV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003413
• ORF Size: 1401 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003413.4
• RefSeq Size: 3939 bp
• RefSeq ORF: 1404 bp
• Locus ID: 7547
• UniProt ID: O60481
• Cytogenetics: Xq26.3
• Protein Families: Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS
• MW: 50.6 kDa
• Gene Summary: This gene encodes a member of the ZIC family of C2H2-type zinc finger proteins. This nuclear protein probably functions as a transcription factor in early stages of left-right body axis formation. Mutations in this gene cause X-linked visceral heterotaxy, which includes congenital heart disease and left-right axis defects in organs. [provided by RefSeq, Jul 2008]