PLEKHM2 (NM_015164) Human Tagged ORF Clone

CAT#: RC220299

PLEKHM2 (Myc-DDK-tagged)-Human pleckstrin homology domain containing, family M (with RUN domain) member 2 (PLEKHM2)

Product Images

Circular map for RC220299

• 

  Western blot validation of overexpression lysate (Cat# LY414756) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC220299 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified PLEKHM2 protein (Cat# TP320299). The protein was produced from HEK293T cells transfected with PLEKHM2 cDNA clone (Cat# RC220299) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PLEKHM2
• Synonyms: SKIP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC220299 representing NM_015164
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCCGGGGGAGGTGAAGGACCGGATCCTGGAGAACATCTCGCTGTCGGTGAAGAAGTTGCAGAGCT
  ATTTTGCTGCATGTGAGGATGAGATCCCTGCCATCCGGAACCATGACAAGGTCCTACAGCGTCTGTGTGA
  GCACCTGGACCACGCCCTGCTGTACGGACTGCAAGACCTCTCCTCTGGCTACTGGGTGCTCGTGGTGCAT
  TTTACTCGGAGAGAGGCCATCAAGCAGATCGAGGTGCTGCAGCACGTGGCCACCAACCTGGGGCGCAGCC
  GTGCCTGGCTGTACCTGGCCCTCAACGAGAACTCCTTGGAGAGCTACCTGCGGTTGTTCCAGGAGAACCT
  GGGCCTGCTGCATAAGTACTACGTCAAGAATGCCCTGGTCTGCAGCCACGATCACCTGACGCTCTTCCTG
  ACCTTGGTGTCCGGGCTAGAGTTCATTCGTTTCGAGCTGGATCTGGATGCCCCTTACCTAGACCTGGCCC
  CCTACATGCCCGACTACTACAAACCTCAGTACCTGCTGGACTTTGAAGACCGCCTTCCCAGCTCGGTCCA
  CGGCTCAGACAGTCTGTCCCTCAACTCTTTCAACTCCGTCACCTCCACCAACCTGGAGTGGGATGACAGT
  GCGATTGCCCCATCTAGTGAGGATTATGATTTTGGAGATGTGTTTCCAGCAGTGCCGTCTGTACCCAGCA
  CAGACTGGGAAGATGGAGACCTCACAGACACGGTCAGTGGTCCCCGCTCCACAGCCTCCGACCTGACCAG
  CAGCAAGGCCTCCACCAGGAGCCCCACCCAGCGCCAGAACCCCTTCAACGAGGAGCCGGCAGAGACTGTG
  TCCTCCTCTGACACCACCCCCGTGCACACCACCTCTCAGGAGAAGGAGGAGGCCCAGGCCCTGGACCCGC
  CGGATGCCTGCACGGAGCTCGAGGTCATCAGGGTCACCAAGAAGAAGAAAATTGGCAAGAAGAAAAAGAG
  CAGATCAGATGAGGAGGCAAGTCCACTCCACCCCGCCTGCAGCCAGAAGAAATGTGCCAAGCAGGGGGAC
  GGTGACAGCCGCAACGGCAGCCCAAGCCTTGGGCGGGACTCGCCAGACACTATGCTTGCCTCCCCCCAGG
  AGGAGGGAGAGGGGCCGAGCAGCACCACGGAGAGCAGCGAGCGCTCCGAGCCGGGCCTGCTGATCCCTGA
  GATGAAGGACACCTCCATGGAGCGCTTGGGGCAGCCCCTGAGCAAGGTTATCGACCAGCTCAACGGGCAG
  CTGGACCCCAGCACCTGGTGCTCCCGTGCTGAGCCCCCAGACCAGTCCTTTCGGACCGGCTCTCCCGGGG
  ATGCCCCGGAGAGGCCGCCGCTTTGCGACTTTAGTGAGGGGCTTTCAGCCCCAATGGACTTCTACCGCTT
  TACCGTCGAGAGTCCAAGCACTGTTACATCAGGTGGCGGCCACCATGACCCTGCAGGGCTTGGCCAACCG
  CTGCATGTTCCTAGTAGCCCTGAGGCTGCTGGCCAAGAAGAAGAGGGAGGAGGAGGAGAGGGACAGACGC
  CTCGGCCCCTAGAGGATACCACGAGGGAGGCTCAGGAGCTGGAGGCCCAGCTGTCCCTGGTCAGGGAGGG
  GCCTGTGTCTGAGCCAGAGCCTGGGACCCAGGAGGTTCTCTGCCAGCTCAAGCGAGACCAGCCCAGCCCG
  TGTCTGAGTAGCGCTGAGGATTCTGGGGTGGATGAGGGACAGGGGAGCCCTTCGGAGATGGTCCATTCCT
  CGGAGTTCAGAGTAGACAACAATCACCTGCTCCTGCTCATGATCCACGTGTTCCGAGAAAACGAAGAGCA
  GCTGTTCAAAATGATCCGGATGAGCACCGGGCACATGGAGGGCAACCTGCAGCTGCTGTACGTGCTGCTC
  ACAGACTGCTATGTCTACCTGCTCCGGAAAGGGGCCACAGAGAAGCCATACCTGGTGGAAGAGGCCGTTT
  CTTACAATGAACTTGACTATGTGTCGGTTGGCCTTGACCAGCAGACGGTGAAGCTGGTGTGCACCAACCG
  CAGGAAGCAGTTTCTGCTGGACACGGCTGATGTGGCGCTGGCTGAGTTCTTTTTGGCTTCTTTGAAGTCA
  GCCATGATCAAAGGCTGTCGAGAACCTCCCTACCCCAGCATCCTGACGGATGCCACCATGGAGAAGCTGG
  CACTGGCCAAATTTGTGGCCCAAGAATCGAAGTGTGAGGCATCTGCTGTCACCGTGCGCTTCTACGGCCT
  TGTGCACTGGGAGGACCCCACAGACGAGTCCCTGGGCCCCACGCCCTGCCACTGCTCACCCCCCGAGGGC
  ACCATCACCAAAGAAGGCATGCTGCACTACAAGGCGGGCACCTCCTACCTGGGCAAGGAACACTGGAAGA
  CGTGCTTCGTGGTGCTCAGCAACGGGATCCTCTACCAGTACCCGGACCGCACCGACGTCATCCCTCTGCT
  CTCGGTGAACATGGGGGGGGAGCAGTGCGGTGGCTGCCGGAGAGCCAACACCACGGATCGGCCCCACGCC
  TTCCAGGTCATTCTCTCCGACCGGCCCTGCCTGGAGCTAAGTGCCGAGAGCGAGGCCGAGATGGCCGAGT
  GGATGCAGCATCTCTGCCAGGCTGTGTCCAAAGGGGTCATCCCCCAGGGCGTAGCTCCCAGCCCCTGCAT
  ACCCTGCTGCCTGGTCCTCACGGATGACCGCCTCTTTACGTGCCATGAGGATTGCCAGACCAGCTTCTTC
  CGCTCTTTGGGCACAGCCAAGCTGGGCGACATCAGCGCCGTCTCCACCGAGCCGGGCAAGGAGTACTGCG
  TCTTGGAGTTCTCCCAGGACAGCCAGCAGCTCCTCCCGCCCTGGGTCATCTACCTGAGCTGCACTTCTGA
  ACTGGACCGATTGCTGTCTGCACTGAACTCTGGGTGGAAAACCATCTATCAGGTGGACCTCCCCCACACG
  GCGATCCAGGAAGCCTCCAACAAGAAGAAATTCGAGGATGCCTTGAGCCTCATCCACAGCGCCTGGCAGC
  GGAGCGACAGTCTCTGCCGCGGCCGAGCCTCCCGAGACCCCTGGTGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC220299 representing NM_015164
  Red=Cloning site Green=Tags(s)
  
  MEPGEVKDRILENISLSVKKLQSYFAACEDEIPAIRNHDKVLQRLCEHLDHALLYGLQDLSSGYWVLVVH
  FTRREAIKQIEVLQHVATNLGRSRAWLYLALNENSLESYLRLFQENLGLLHKYYVKNALVCSHDHLTLFL
  TLVSGLEFIRFELDLDAPYLDLAPYMPDYYKPQYLLDFEDRLPSSVHGSDSLSLNSFNSVTSTNLEWDDS
  AIAPSSEDYDFGDVFPAVPSVPSTDWEDGDLTDTVSGPRSTASDLTSSKASTRSPTQRQNPFNEEPAETV
  SSSDTTPVHTTSQEKEEAQALDPPDACTELEVIRVTKKKKIGKKKKSRSDEEASPLHPACSQKKCAKQGD
  GDSRNGSPSLGRDSPDTMLASPQEEGEGPSSTTESSERSEPGLLIPEMKDTSMERLGQPLSKVIDQLNGQ
  LDPSTWCSRAEPPDQSFRTGSPGDAPERPPLCDFSEGLSAPMDFYRFTVESPSTVTSGGGHHDPAGLGQP
  LHVPSSPEAAGQEEEGGGGEGQTPRPLEDTTREAQELEAQLSLVREGPVSEPEPGTQEVLCQLKRDQPSP
  CLSSAEDSGVDEGQGSPSEMVHSSEFRVDNNHLLLLMIHVFRENEEQLFKMIRMSTGHMEGNLQLLYVLL
  TDCYVYLLRKGATEKPYLVEEAVSYNELDYVSVGLDQQTVKLVCTNRRKQFLLDTADVALAEFFLASLKS
  AMIKGCREPPYPSILTDATMEKLALAKFVAQESKCEASAVTVRFYGLVHWEDPTDESLGPTPCHCSPPEG
  TITKEGMLHYKAGTSYLGKEHWKTCFVVLSNGILYQYPDRTDVIPLLSVNMGGEQCGGCRRANTTDRPHA
  FQVILSDRPCLELSAESEAEMAEWMQHLCQAVSKGVIPQGVAPSPCIPCCLVLTDDRLFTCHEDCQTSFF
  RSLGTAKLGDISAVSTEPGKEYCVLEFSQDSQQLLPPWVIYLSCTSELDRLLSALNSGWKTIYQVDLPHT
  AIQEASNKKKFEDALSLIHSAWQRSDSLCRGRASRDPWC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015164
• ORF Size: 3057 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015164.4
• RefSeq Size: 4231 bp
• RefSeq ORF: 3060 bp
• Locus ID: 23207
• UniProt ID: Q8IWE5
• Cytogenetics: 1p36.21
• Protein Families: Druggable Genome
• MW: 112.6 kDa
• Gene Summary: This gene encodes a protein that binds the plus-end directed microtubule motor protein kinesin, together with the lysosomal GTPase Arl8, and is required for lysosomes to distribute away from the microtubule-organizing center. The encoded protein belongs to the multisubunit BLOC-one-related complex that regulates lysosome positioning. It binds a Salmonella effector protein called Salmonella induced filament A and is a critical host determinant in Salmonella pathogenesis. It has a domain architecture consisting of an N-terminal RPIP8, UNC-14, and NESCA (RUN) domain that binds kinesin-1 as well as the lysosomal GTPase Arl8, and a C-terminal pleckstrin homology domain that binds the Salmonella induced filament A effector protein. Naturally occurring mutations in this gene lead to abnormal localization of lysosomes, impaired autophagy flux and are associated with recessive dilated cardiomyopathy and left ventricular noncompaction. [provided by RefSeq, Feb 2017]