PLEKHM2 (NM_015164) Human Tagged ORF Clone

SKU RC220299

PLEKHM2 (Myc-DDK-tagged)-Human pleckstrin homology domain containing, family M (with RUN domain) member 2 (PLEKHM2)

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: PLEKHM2
• Synonyms: SKIP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC220299 representing NM_015164; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGAGCCGGGGGAGGTGAAGGACCGGATCCTGGAGAACATCTCGCTGTCGGTGAAGAAGTTGCAGAGCT; ATTTTGCTGCATGTGAGGATGAGATCCCTGCCATCCGGAACCATGACAAGGTCCTACAGCGTCTGTGTGA; GCACCTGGACCACGCCCTGCTGTACGGACTGCAAGACCTCTCCTCTGGCTACTGGGTGCTCGTGGTGCAT; TTTACTCGGAGAGAGGCCATCAAGCAGATCGAGGTGCTGCAGCACGTGGCCACCAACCTGGGGCGCAGCC; GTGCCTGGCTGTACCTGGCCCTCAACGAGAACTCCTTGGAGAGCTACCTGCGGTTGTTCCAGGAGAACCT; GGGCCTGCTGCATAAGTACTACGTCAAGAATGCCCTGGTCTGCAGCCACGATCACCTGACGCTCTTCCTG; ACCTTGGTGTCCGGGCTAGAGTTCATTCGTTTCGAGCTGGATCTGGATGCCCCTTACCTAGACCTGGCCC; CCTACATGCCCGACTACTACAAACCTCAGTACCTGCTGGACTTTGAAGACCGCCTTCCCAGCTCGGTCCA; CGGCTCAGACAGTCTGTCCCTCAACTCTTTCAACTCCGTCACCTCCACCAACCTGGAGTGGGATGACAGT; GCGATTGCCCCATCTAGTGAGGATTATGATTTTGGAGATGTGTTTCCAGCAGTGCCGTCTGTACCCAGCA; CAGACTGGGAAGATGGAGACCTCACAGACACGGTCAGTGGTCCCCGCTCCACAGCCTCCGACCTGACCAG; CAGCAAGGCCTCCACCAGGAGCCCCACCCAGCGCCAGAACCCCTTCAACGAGGAGCCGGCAGAGACTGTG; TCCTCCTCTGACACCACCCCCGTGCACACCACCTCTCAGGAGAAGGAGGAGGCCCAGGCCCTGGACCCGC; CGGATGCCTGCACGGAGCTCGAGGTCATCAGGGTCACCAAGAAGAAGAAAATTGGCAAGAAGAAAAAGAG; CAGATCAGATGAGGAGGCAAGTCCACTCCACCCCGCCTGCAGCCAGAAGAAATGTGCCAAGCAGGGGGAC; GGTGACAGCCGCAACGGCAGCCCAAGCCTTGGGCGGGACTCGCCAGACACTATGCTTGCCTCCCCCCAGG; AGGAGGGAGAGGGGCCGAGCAGCACCACGGAGAGCAGCGAGCGCTCCGAGCCGGGCCTGCTGATCCCTGA; GATGAAGGACACCTCCATGGAGCGCTTGGGGCAGCCCCTGAGCAAGGTTATCGACCAGCTCAACGGGCAG; CTGGACCCCAGCACCTGGTGCTCCCGTGCTGAGCCCCCAGACCAGTCCTTTCGGACCGGCTCTCCCGGGG; ATGCCCCGGAGAGGCCGCCGCTTTGCGACTTTAGTGAGGGGCTTTCAGCCCCAATGGACTTCTACCGCTT; TACCGTCGAGAGTCCAAGCACTGTTACATCAGGTGGCGGCCACCATGACCCTGCAGGGCTTGGCCAACCG; CTGCATGTTCCTAGTAGCCCTGAGGCTGCTGGCCAAGAAGAAGAGGGAGGAGGAGGAGAGGGACAGACGC; CTCGGCCCCTAGAGGATACCACGAGGGAGGCTCAGGAGCTGGAGGCCCAGCTGTCCCTGGTCAGGGAGGG; GCCTGTGTCTGAGCCAGAGCCTGGGACCCAGGAGGTTCTCTGCCAGCTCAAGCGAGACCAGCCCAGCCCG; TGTCTGAGTAGCGCTGAGGATTCTGGGGTGGATGAGGGACAGGGGAGCCCTTCGGAGATGGTCCATTCCT; CGGAGTTCAGAGTAGACAACAATCACCTGCTCCTGCTCATGATCCACGTGTTCCGAGAAAACGAAGAGCA; GCTGTTCAAAATGATCCGGATGAGCACCGGGCACATGGAGGGCAACCTGCAGCTGCTGTACGTGCTGCTC; ACAGACTGCTATGTCTACCTGCTCCGGAAAGGGGCCACAGAGAAGCCATACCTGGTGGAAGAGGCCGTTT; CTTACAATGAACTTGACTATGTGTCGGTTGGCCTTGACCAGCAGACGGTGAAGCTGGTGTGCACCAACCG; CAGGAAGCAGTTTCTGCTGGACACGGCTGATGTGGCGCTGGCTGAGTTCTTTTTGGCTTCTTTGAAGTCA; GCCATGATCAAAGGCTGTCGAGAACCTCCCTACCCCAGCATCCTGACGGATGCCACCATGGAGAAGCTGG; CACTGGCCAAATTTGTGGCCCAAGAATCGAAGTGTGAGGCATCTGCTGTCACCGTGCGCTTCTACGGCCT; TGTGCACTGGGAGGACCCCACAGACGAGTCCCTGGGCCCCACGCCCTGCCACTGCTCACCCCCCGAGGGC; ACCATCACCAAAGAAGGCATGCTGCACTACAAGGCGGGCACCTCCTACCTGGGCAAGGAACACTGGAAGA; CGTGCTTCGTGGTGCTCAGCAACGGGATCCTCTACCAGTACCCGGACCGCACCGACGTCATCCCTCTGCT; CTCGGTGAACATGGGGGGGGAGCAGTGCGGTGGCTGCCGGAGAGCCAACACCACGGATCGGCCCCACGCC; TTCCAGGTCATTCTCTCCGACCGGCCCTGCCTGGAGCTAAGTGCCGAGAGCGAGGCCGAGATGGCCGAGT; GGATGCAGCATCTCTGCCAGGCTGTGTCCAAAGGGGTCATCCCCCAGGGCGTAGCTCCCAGCCCCTGCAT; ACCCTGCTGCCTGGTCCTCACGGATGACCGCCTCTTTACGTGCCATGAGGATTGCCAGACCAGCTTCTTC; CGCTCTTTGGGCACAGCCAAGCTGGGCGACATCAGCGCCGTCTCCACCGAGCCGGGCAAGGAGTACTGCG; TCTTGGAGTTCTCCCAGGACAGCCAGCAGCTCCTCCCGCCCTGGGTCATCTACCTGAGCTGCACTTCTGA; ACTGGACCGATTGCTGTCTGCACTGAACTCTGGGTGGAAAACCATCTATCAGGTGGACCTCCCCCACACG; GCGATCCAGGAAGCCTCCAACAAGAAGAAATTCGAGGATGCCTTGAGCCTCATCCACAGCGCCTGGCAGC; GGAGCGACAGTCTCTGCCGCGGCCGAGCCTCCCGAGACCCCTGGTGC; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC220299 representing NM_015164; Red=Cloning site Green=Tags(s); ; MEPGEVKDRILENISLSVKKLQSYFAACEDEIPAIRNHDKVLQRLCEHLDHALLYGLQDLSSGYWVLVVH; FTRREAIKQIEVLQHVATNLGRSRAWLYLALNENSLESYLRLFQENLGLLHKYYVKNALVCSHDHLTLFL; TLVSGLEFIRFELDLDAPYLDLAPYMPDYYKPQYLLDFEDRLPSSVHGSDSLSLNSFNSVTSTNLEWDDS; AIAPSSEDYDFGDVFPAVPSVPSTDWEDGDLTDTVSGPRSTASDLTSSKASTRSPTQRQNPFNEEPAETV; SSSDTTPVHTTSQEKEEAQALDPPDACTELEVIRVTKKKKIGKKKKSRSDEEASPLHPACSQKKCAKQGD; GDSRNGSPSLGRDSPDTMLASPQEEGEGPSSTTESSERSEPGLLIPEMKDTSMERLGQPLSKVIDQLNGQ; LDPSTWCSRAEPPDQSFRTGSPGDAPERPPLCDFSEGLSAPMDFYRFTVESPSTVTSGGGHHDPAGLGQP; LHVPSSPEAAGQEEEGGGGEGQTPRPLEDTTREAQELEAQLSLVREGPVSEPEPGTQEVLCQLKRDQPSP; CLSSAEDSGVDEGQGSPSEMVHSSEFRVDNNHLLLLMIHVFRENEEQLFKMIRMSTGHMEGNLQLLYVLL; TDCYVYLLRKGATEKPYLVEEAVSYNELDYVSVGLDQQTVKLVCTNRRKQFLLDTADVALAEFFLASLKS; AMIKGCREPPYPSILTDATMEKLALAKFVAQESKCEASAVTVRFYGLVHWEDPTDESLGPTPCHCSPPEG; TITKEGMLHYKAGTSYLGKEHWKTCFVVLSNGILYQYPDRTDVIPLLSVNMGGEQCGGCRRANTTDRPHA; FQVILSDRPCLELSAESEAEMAEWMQHLCQAVSKGVIPQGVAPSPCIPCCLVLTDDRLFTCHEDCQTSFF; RSLGTAKLGDISAVSTEPGKEYCVLEFSQDSQQLLPPWVIYLSCTSELDRLLSALNSGWKTIYQVDLPHT; AIQEASNKKKFEDALSLIHSAWQRSDSLCRGRASRDPWC; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015164
• ORF Size: 3057 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_015164.4
• RefSeq Size: 4231 bp
• RefSeq ORF: 3060 bp
• Locus ID: 23207
• UniProt ID: Q8IWE5
• Cytogenetics: 1p36.21
• Protein Families: Druggable Genome
• MW: 112.6 kDa
• Summary: This gene encodes a protein that binds the plus-end directed microtubule motor protein kinesin, together with the lysosomal GTPase Arl8, and is required for lysosomes to distribute away from the microtubule-organizing center. The encoded protein belongs to the multisubunit BLOC-one-related complex that regulates lysosome positioning. It binds a Salmonella effector protein called Salmonella induced filament A and is a critical host determinant in Salmonella pathogenesis. It has a domain architecture consisting of an N-terminal RPIP8, UNC-14, and NESCA (RUN) domain that binds kinesin-1 as well as the lysosomal GTPase Arl8, and a C-terminal pleckstrin homology domain that binds the Salmonella induced filament A effector protein. Naturally occurring mutations in this gene lead to abnormal localization of lysosomes, impaired autophagy flux and are associated with recessive dilated cardiomyopathy and left ventricular noncompaction. [provided by RefSeq, Feb 2017]