PPP2R2B (NM_004576) Human Tagged ORF Clone

CAT#: RC220271

PPP2R2B (Myc-DDK-tagged)-Human protein phosphatase 2, regulatory subunit B, beta (PPP2R2B), transcript variant 1

Product Images

Circular map for RC220271

• 

  Western blot validation of overexpression lysate (Cat# LY426765) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC225726 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PPP2R2B
• Synonyms: B55BETA; PP2AB55BETA; PP2ABBETA; PP2APR55B; PP2APR55BETA; PR2AB55BETA; PR2ABBETA; PR2APR55BETA; PR52B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC220271 representing NM_004576
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGAGGACATTGATACCCGCAAAATCAACAACAGTTTCCTGCGCGACCACAGCTATGCGACCGAAG
  CTGACATTATCTCTACGGTAGAATTCAACCACACGGGAGAATTACTAGCGACAGGGGACAAGGGGGGTCG
  GGTTGTAATATTTCAACGAGAGCAGGAGAGTAAAAATCAGGTTCATCGTAGGGGTGAATACAATGTTTAC
  AGCACATTCCAGAGCCATGAACCCGAGTTCGATTACCTGAAGAGTTTAGAAATAGAAGAAAAAATCAATA
  AAATAAGATGGCTCCCCCAGCAGAATGCAGCTTACTTTCTTCTGTCTACTAATGATAAAACTGTGAAGCT
  GTGGAAAGTCAGCGAGCGTGATAAGAGGCCAGAAGGCTACAATCTGAAAGATGAGGAGGGCCGGCTCCGG
  GATCCTGCCACCATCACAACCCTGCGGGTGCCTGTCCTGAGACCCATGGACCTGATGGTGGAGGCCACCC
  CACGAAGAGTATTTGCCAACGCACACACATATCACATCAACTCCATATCTGTCAACAGCGACTATGAAAC
  CTACATGTCCGCTGATGACCTGAGGATTAACCTATGGAACTTTGAAATAACCAATCAAAGTTTTAATATT
  GTGGACATTAAGCCAGCCAACATGGAGGAGCTCACGGAGGTGATCACAGCAGCCGAGTTCCACCCCCATC
  ATTGCAACACCTTCGTGTACAGCAGCAGCAAAGGGACAATCCGGCTGTGTGACATGCGGGCATCTGCCCT
  GTGTGACAGGCACACCAAATTTTTTGAAGAGCCGGAAGATCCAAGCAACAGATCATTTTTCTCTGAAATT
  ATCTCTTCGATTTCGGATGTGAAGTTCAGCCACAGTGGGAGGTATATCATGACCAGGGACTACTTGACCG
  TCAAAGTCTGGGATCTCAACATGGAAAACCGCCCCATCGAGACTTACCAGGTTCATGACTACCTCCGCAG
  CAAGCTGTGTTCCCTCTATGAAAATGACTGCATTTTTGATAAATTTGAGTGTGTGTGGAATGGGTCAGAC
  AGTGTCATCATGACAGGCTCCTACAACAACTTCTTCAGGATGTTCGACAGAAACACCAAGCGTGATGTGA
  CCCTTGAGGCTTCGAGGGAAAACAGCAAGCCCCGGGCTATCCTCAAACCCCGAAAAGTGTGTGTGGGGGG
  CAAGCGGAGAAAAGACGAGATCAGTGTCGACAGTCTGGACTTTAGCAAAAAGATCTTGCATACAGCTTGG
  CATCCTTCAGAAAATATTATAGCAGTGGCGGCTACAAATAACCTATATATATTCCAGGACAAGGTTAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC220271 representing NM_004576
  Red=Cloning site Green=Tags(s)
  
  MEEDIDTRKINNSFLRDHSYATEADIISTVEFNHTGELLATGDKGGRVVIFQREQESKNQVHRRGEYNVY
  STFQSHEPEFDYLKSLEIEEKINKIRWLPQQNAAYFLLSTNDKTVKLWKVSERDKRPEGYNLKDEEGRLR
  DPATITTLRVPVLRPMDLMVEATPRRVFANAHTYHINSISVNSDYETYMSADDLRINLWNFEITNQSFNI
  VDIKPANMEELTEVITAAEFHPHHCNTFVYSSSKGTIRLCDMRASALCDRHTKFFEEPEDPSNRSFFSEI
  ISSISDVKFSHSGRYIMTRDYLTVKVWDLNMENRPIETYQVHDYLRSKLCSLYENDCIFDKFECVWNGSD
  SVIMTGSYNNFFRMFDRNTKRDVTLEASRENSKPRAILKPRKVCVGGKRRKDEISVDSLDFSKKILHTAW
  HPSENIIAVAATNNLYIFQDKVN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004576
• ORF Size: 1329 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004576.2, NP_004567.1
• RefSeq Size: 2300 bp
• RefSeq ORF: 1331 bp
• Locus ID: 5521
• Cytogenetics: 5q32
• Domains: WD40
• Protein Families: Druggable Genome, Phosphatase
• Protein Pathways: Tight junction
• MW: 51.5 kDa
• Gene Summary: The product of this gene belongs to the phosphatase 2 regulatory subunit B family. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity. This gene encodes a beta isoform of the regulatory subunit B55 subfamily. Defects in this gene cause autosomal dominant spinocerebellar ataxia 12 (SCA12), a disease caused by degeneration of the cerebellum, sometimes involving the brainstem and spinal cord, and in resulting in poor coordination of speech and body movements. Multiple alternatively spliced variants, which encode different isoforms, have been identified for this gene. The 5' UTR of some of these variants includes a CAG trinucleotide repeat sequence (7-28 copies) that can be expanded to 55-78 copies in cases of SCA12. [provided by RefSeq, Jul 2016]