KBTBD11 (NM_014867) Human Tagged ORF Clone

CAT#: RC220263

KBTBD11 (Myc-DDK-tagged)-Human kelch repeat and BTB (POZ) domain containing 11 (KBTBD11)

Product Images

Circular map for RC220263

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  Western blot validation of overexpression lysate (Cat# LY414975) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC220263 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified KBTBD11 protein (Cat# TP320263). The protein was produced from HEK293T cells transfected with KBTBD11 cDNA clone (Cat# RC220263) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: KBTBD11
• Synonyms: KLHDC7C
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC220263 representing NM_014867
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCACGCGGTGGCCCCCTGCGTCCTCTACCCAGGGACTGAGCCCGGGGCTGCCGGGGAGAGCGAGA
  GCGAGGGCGCCGCGTCCCCGGCGCAGACACCCTGCAGTCTCGGCGCGTCCCTGTGCTTCAGCTCCGGGGA
  AGAGTCCCCGCCGCAGTCCCTCGCCTCAGCGGCGGAAGGCGCGGCCACCTCCCCGCCCTCCAGCGGTGGC
  CCGCGGGTGGTGGAGCGGCAGTGGGAGGCCGGCAGCGCGGGCGCCGCGTCCCCGGAGGAGCTCGCGTCCC
  CTGAGGAGCGCGCGTGCCCGGAAGAGCCCGCGGCGCCGTCCCCCGAACCGCGCGTTTGGCTTGAGGACCC
  CGCGTCCCCCGAGGAGCCCGGGGAGCCCGCGCCCGTACCCCCGGGGTTCGGGGCGGTGTACGGGGAGCCG
  GACCTGGTGCTGGAGGTGTCGGGGCGCCGGCTGCGCGCGCACAAGGCGGTGCTGGCGGCGCGCAGCGACT
  ACTTCCGCGCGCGCGCGTCGCGGGACGTGCTGCGGGTGCAGGGAGTGAGCCTGACGGCGCTGCGGCTGCT
  CCTCGCCGACGCCTACAGCGGGCGCATGGCGGGCGTGCGGCCCGACAACGTGGCCGAGGTGGTGGCCGGC
  GCGCGCCGCCTGCAGCTGCCCGGCGCCGCGCAGCGCGCCACCGACGCCGTGGGGCCGCAGCTGAGCCTGG
  CCAACTGCTACGAGGTCCTGAGCGCGGCCAAGCGGCAGCGGCTGAACGAGCTGCGCGACGCCGCCTACTG
  CTTCATGAGCGACCACTATCTGGAGGTGCTGCGCGAGCCCGCCGTGTTCGGCCGCCTGTCGGGCGCAGAG
  CGGGACCTGCTGCTGCGCCGCCGCCTGCGCGCCGGCCGCGCCCACCTCTTGGCCGCGGCGCTCGGGCCGG
  CGGGGGAGCGCGCGGGCAGCCGGCCTCAGAGCCCCTCGGGGGACGCGGACGCGCGCGGGGACGCGGCCGT
  CTACTGCTTCCACGCGGCGGCCGGAGAGTGGCGCGAGCTGACGCGGCTGCCCGAGGGCGCGCCGGCGCGG
  GGCTGCGGCCTGTGCGTCCTCTACAACTACCTCTTCGTGGCGGGCGGCGTGGCGCCCGCGGGCCCCGACG
  GCCGCGCGCGCCCGTCCGACCAGGTCTTCTGCTACAACCCGGCCACGGACAGCTGGAGCGCCGTGAGGCC
  CCTGCGCCAGGCGCGCTCGCAGCTGCGGCTGCTGGCCCTGGACGGTCACCTCTACGCCGTGGGCGGCGAG
  TGCCTGCTCAGCGTGGAGCGCTACGACCCGCGCGCCGACCGCTGGGCCCCCGTGGCGCCGCTGCCCCGGG
  GCGCCTTCGCCGTGGCGCATGAGGCCACCACCTGCCACGGCGAGATCTACGTGTCCGGGGGCTCCCTCTT
  CTATCGCCTGCTCAAGTATGACCCGCGGCGCGACGAGTGGCAGGAGTGCCCGTGCAGCAGCAGCCGCGAG
  CGCTCGGCCGACATGGTGGCTCTCGACGGCTTCATCTACCGCTTCGATCTGAGCGGCAGCCGCGGCGAGG
  CGCAGGCGGCGGGGCCGAGCGGGGTCAGCGTGTCCCGATACCACTGCCTGGCCAAGCAGTGGAGCCCGTG
  CGTCGCGCCCCTGCGCCTCCCCGGCGGCCCCACGGGCCTGCAGCCCTTCCGCTGCGCCGCCCTGGACGGC
  GCCATCTACTGCGTGAGCCGCGCGGGCACCTGGCGCTTCCAGCCTGCCCGGGAAGGCGAGGCCGGCGGCG
  ACGCAGGCCAGGGCGGCGGCTTCGAGGCGCTGGGCGCCCCCTTGGACGTCCGGGGTGTGCTCATCCCGTT
  CGCTCTCAGCCTGCCTGAGAAGCCGCCCCGAGGGGAGCAGGGCGCCCCG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC220263 representing NM_014867
  Red=Cloning site Green=Tags(s)
  
  MEHAVAPCVLYPGTEPGAAGESESEGAASPAQTPCSLGASLCFSSGEESPPQSLASAAEGAATSPPSSGG
  PRVVERQWEAGSAGAASPEELASPEERACPEEPAAPSPEPRVWLEDPASPEEPGEPAPVPPGFGAVYGEP
  DLVLEVSGRRLRAHKAVLAARSDYFRARASRDVLRVQGVSLTALRLLLADAYSGRMAGVRPDNVAEVVAG
  ARRLQLPGAAQRATDAVGPQLSLANCYEVLSAAKRQRLNELRDAAYCFMSDHYLEVLREPAVFGRLSGAE
  RDLLLRRRLRAGRAHLLAAALGPAGERAGSRPQSPSGDADARGDAAVYCFHAAAGEWRELTRLPEGAPAR
  GCGLCVLYNYLFVAGGVAPAGPDGRARPSDQVFCYNPATDSWSAVRPLRQARSQLRLLALDGHLYAVGGE
  CLLSVERYDPRADRWAPVAPLPRGAFAVAHEATTCHGEIYVSGGSLFYRLLKYDPRRDEWQECPCSSSRE
  RSADMVALDGFIYRFDLSGSRGEAQAAGPSGVSVSRYHCLAKQWSPCVAPLRLPGGPTGLQPFRCAALDG
  AIYCVSRAGTWRFQPAREGEAGGDAGQGGGFEALGAPLDVRGVLIPFALSLPEKPPRGEQGAP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014867
• ORF Size: 1869 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014867.3
• RefSeq Size: 6706 bp
• RefSeq ORF: 1872 bp
• Locus ID: 9920
• UniProt ID: O94819
• Cytogenetics: 8p23.3
• Domains: BTB, Kelch
• MW: 65.5 kDa