PTPRN (NM_002846) Human Tagged ORF Clone

CAT#: RC220230

PTPRN (Myc-DDK-tagged)-Human protein tyrosine phosphatase, receptor type, N (PTPRN), transcript variant 1

Product Images

Circular map for RC220230

• 

  Western blot validation of overexpression lysate (Cat# LY419074) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC220230 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PTPRN
• Synonyms: IA-2; IA-2/PTP; IA2; ICA512; R-PTP-N
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC220230 representing NM_002846
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGGCGCCCGCGGCGGCCTGGGGGTCTCGGGGGATCCGGGGGTCTCCGGCTGCTCCTCTGCCTCCTGC
  TGCTGAGCAGCCGCCCGGGGGGCTGCAGCGCCGTTAGTGCCCACGGCTGTCTATTTGACCGCAGGCTCTG
  CTCTCACCTGGAAGTCTGTATTCAGGATGGCTTGTTTGGGCAGTGCCAGGTGGGAGTGGGGCAGGCCCGG
  CCCCTTTTGCAAGTCACCTCCCCAGTTCTCCAACGCTTACAAGGTGTGCTCCGACAACTCATGTCCCAAG
  GATTGTCCTGGCACGATGACCTCACCCAGTATGTGATCTCTCAGGAGATGGAGCGCATCCCCAGGCTTCG
  CCCCCCAGAGCCCCGTCCAAGGGACAGGTCTGGCTTGGCACCCAAGAGACCTGGTCCTGCTGGAGAGCTG
  CTTTTACAGGACATCCCCACTGGCTCCGCCCCTGCTGCCCAGCATCGGCTTCCACAACCACCAGTGGGCA
  AAGGTGGAGCTGGGGCCAGCTCCTCTCTGTCCCCTCTGCAGGCTGAGCTGCTCCCGCCTCTCTTGGAGCA
  CCTGCTGCTGCCCCCACAGCCTCCCCACCCTTCACTGAGTTACGAACCTGCCTTGCTGCAGCCCTACCTG
  TTCCACCAGTTTGGCTCCCGTGATGGCTCCAGGGTCTCAGAGGGCTCCCCAGGGATGGTCAGTGTCGGCC
  CCCTGCCCAAGGCTGAAGCCCCTGCCCTCTTCAGCAGAACTGCCTCCAAGGGCATATTTGGGGACCACCC
  TGGCCACTCCTACGGGGACCTTCCAGGGCCTTCACCTGCCCAGCTTTTTCAAGACTCTGGGCTGCTCTAT
  CTGGCCCAGGAGTTGCCAGCACCCAGCAGGGCCAGGGTGCCAAGGCTGCCAGAGCAAGGGAGCAGCAGCC
  GGGCAGAGGACTCCCCAGAGGGCTATGAGAAGGAAGGACTAGGGGATCGTGGAGAGAAGCCTGCTTCCCC
  AGCTGTGCAGCCAGATGCGGCTCTGCAGAGGCTGGCCGCTGTGCTGGCGGGCTATGGGGTAGAGCTGCGT
  CAGCTGACCCCTGAGCAGCTCTCCACACTCCTGACCCTGCTGCAGCTACTGCCCAAGGGTGCAGGAAGAA
  ATCCGGGAGGGGTTGTAAATGTTGGAGCTGATATCAAGAAAACAATGGAGGGGCCGGTGGAGGGCAGAGA
  CACAGCAGAGCTTCCAGCCCGCACATCCCCCATGCCTGGACACCCCACTGCCAGCCCTACCTCCAGTGAA
  GTCCAGCAGGTGCCAAGCCCTGTCTCCTCTGAGCCTCCCAAAGCTGCCAGACCCCCTGTGACACCTGTCC
  TGCTAGAGAAGAAAAGCCCACTGGGCCAGAGCCAGCCCACGGTGGCAGGACAGCCCTCAGCCCGCCCAGC
  AGCAGAGGAATATGGCTACATCGTCACTGATCAGAAGCCCCTGAGCCTGGCTGCAGGAGTGAAGCTGCTG
  GAGATCCTGGCTGAGCATGTGCACATGTCCTCAGGCAGCTTCATCAACATCAGTGTGGTGGGACCAGCCC
  TCACCTTCCGCATCCGGCACAATGAGCAGAACCTGTCTTTGGCTGATGTGACCCAACAAGCAGGGCTGGT
  GAAGTCTGAACTGGAAGCACAGACAGGGCTCCAAATCTTGCAGACAGGAGTGGGACAGAGGGAGGAGGCA
  GCTGCAGTCCTTCCCCAAACTGCGCACAGCACCTCACCCATGCGCTCAGTGCTGCTCACTCTGGTGGCCC
  TGGCAGGTGTGGCTGGGCTGCTGGTGGCTCTGGCTGTGGCTCTGTGTGTGCGGCAGCATGCGCGGCAGCA
  AGACAAGGAGCGCCTGGCAGCCCTGGGGCCTGAGGGGGCCCATGGTGACACTACCTTTGAGTACCAGGAC
  CTGTGCCGCCAGCACATGGCCACGAAGTCCTTGTTCAACCGGGCAGAGGGTCCACCGGAGCCTTCACGGG
  TGAGCAGTGTGTCCTCCCAGTTCAGCGACGCAGCCCAGGCCAGCCCCAGCTCCCACAGCAGCACCCCGTC
  CTGGTGCGAGGAGCCGGCCCAAGCCAACATGGACATCTCCACGGGACACATGATTCTGGCATACATGGAG
  GATCACCTGCGGAACCGGGACCGCCTTGCCAAGGAGTGGCAGGCCCTCTGTGCCTACCAAGCAGAGCCAA
  ACACCTGTGCCACCGCGCAGGGGGAGGGCAACATCAAAAAGAACCGGCATCCTGACTTCCTGCCCTATGA
  CCATGCCCGCATAAAACTGAAGGTGGAGAGCAGCCCTTCTCGGAGCGATTACATCAACGCCAGCCCCATT
  ATTGAGCATGACCCTCGGATGCCAGCCTACATAGCCACGCAGGGCCCGCTGTCCCATACCATCGCAGACT
  TCTGGCAGATGGTGTGGGAGAGCGGCTGCACCGTCATCGTCATGCTGACCCCGCTGGTGGAGGATGGTGT
  CAAGCAGTGTGACCGCTACTGGCCAGATGAGGGTGCCTCCCTCTACCACGTATATGAGGTGAACCTGGTG
  TCGGAGCACATCTGGTGCGAGGACTTTCTGGTGCGGAGCTTCTACCTGAAGAACGTGCAGACCCAGGAGA
  CGCGCACGCTCACGCAGTTCCACTTCCTCAGCTGGCCGGCAGAGGGCACACCGGCCTCCACGCGGCCCCT
  GCTGGACTTCCGCAGGAAGGTGAACAAGTGCTACCGGGGCCGCTCCTGCCCCATCATCGTGCACTGCAGT
  GATGGTGCGGGGAGGACCGGCACCTACATCCTCATCGACATGGTCCTGAACCGCATGGCAAAAGGAGTGA
  AGGAGATTGACATCGCTGCCACCCTGGAGCATGTCCGTGACCAGCGGCCTGGCCTTGTCCGCTCTAAGGA
  CCAGTTTGAATTTGCCCTGACAGCCGTGGCGGAGGAAGTGAATGCCATCCTCAAGGCCCTGCCCCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC220230 representing NM_002846
  Red=Cloning site Green=Tags(s)
  
  MRRPRRPGGLGGSGGLRLLLCLLLLSSRPGGCSAVSAHGCLFDRRLCSHLEVCIQDGLFGQCQVGVGQAR
  PLLQVTSPVLQRLQGVLRQLMSQGLSWHDDLTQYVISQEMERIPRLRPPEPRPRDRSGLAPKRPGPAGEL
  LLQDIPTGSAPAAQHRLPQPPVGKGGAGASSSLSPLQAELLPPLLEHLLLPPQPPHPSLSYEPALLQPYL
  FHQFGSRDGSRVSEGSPGMVSVGPLPKAEAPALFSRTASKGIFGDHPGHSYGDLPGPSPAQLFQDSGLLY
  LAQELPAPSRARVPRLPEQGSSSRAEDSPEGYEKEGLGDRGEKPASPAVQPDAALQRLAAVLAGYGVELR
  QLTPEQLSTLLTLLQLLPKGAGRNPGGVVNVGADIKKTMEGPVEGRDTAELPARTSPMPGHPTASPTSSE
  VQQVPSPVSSEPPKAARPPVTPVLLEKKSPLGQSQPTVAGQPSARPAAEEYGYIVTDQKPLSLAAGVKLL
  EILAEHVHMSSGSFINISVVGPALTFRIRHNEQNLSLADVTQQAGLVKSELEAQTGLQILQTGVGQREEA
  AAVLPQTAHSTSPMRSVLLTLVALAGVAGLLVALAVALCVRQHARQQDKERLAALGPEGAHGDTTFEYQD
  LCRQHMATKSLFNRAEGPPEPSRVSSVSSQFSDAAQASPSSHSSTPSWCEEPAQANMDISTGHMILAYME
  DHLRNRDRLAKEWQALCAYQAEPNTCATAQGEGNIKKNRHPDFLPYDHARIKLKVESSPSRSDYINASPI
  IEHDPRMPAYIATQGPLSHTIADFWQMVWESGCTVIVMLTPLVEDGVKQCDRYWPDEGASLYHVYEVNLV
  SEHIWCEDFLVRSFYLKNVQTQETRTLTQFHFLSWPAEGTPASTRPLLDFRRKVNKCYRGRSCPIIVHCS
  DGAGRTGTYILIDMVLNRMAKGVKEIDIAATLEHVRDQRPGLVRSKDQFEFALTAVAEEVNAILKALPQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002846
• ORF Size: 2937 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002846.4
• RefSeq Size: 3649 bp
• RefSeq ORF: 2940 bp
• Locus ID: 5798
• UniProt ID: Q16849
• Cytogenetics: 2q35
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Type I diabetes mellitus
• MW: 105.85 kDa
• Gene Summary: The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP possesses an extracellular region, a single transmembrane region, and a single catalytic domain, and thus represents a receptor-type PTP. This PTP was found to be an autoantigen that is reactive with insulin-dependent diabetes mellitus (IDDM) patient sera, and thus may be a potential target of autoimmunity in diabetes mellitus. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Dec 2010]