IL16 (NM_004513) Human Tagged ORF Clone

CAT#: RC220031

IL16 (Myc-DDK-tagged)-Human interleukin 16 (lymphocyte chemoattractant factor) (IL16), transcript variant 1

Product Images

Circular map for RC220031

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  Western blot validation of overexpression lysate (Cat# LY401436) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC220031 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified IL16 protein (Cat# TP320031). The protein was produced from HEK293T cells transfected with IL16 cDNA clone (Cat# RC220031) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: IL16
• Synonyms: LCF; NIL16; prIL-16; PRIL16
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC220031 representing NM_004513
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACTATAGCTTTGATACCACAGCCGAAGACCCTTGGGTTAGGATTTCTGACTGCATCAAAAACTTAT
  TTAGCCCCATCATGAGTGAGAACCATGGCCACATGCCTCTACAGCCCAATGCCAGCCTGAATGAAGAAGA
  AGGGACACAGGGCCACCCAGATGGGACCCCACCAAAGCTGGACACCGCCAATGGCACTCCCAAAGTTTAC
  AAGTCAGCAGACAGCAGCACTGTGAAGAAAGGTCCTCCTGTGGCTCCCAAGCCAGCCTGGTTTCGCCAAA
  GCTTGAAAGGTTTGAGGAATCGTGCTTCAGACCCAAGAGGGCTCCCTGATCCTGCCTTGTCCACCCAGCC
  AGCACCTGCTTCCAGGGAGCACCTAGGATCACACATCCGGGCCTCCTCCTCCTCCTCCTCCATCAGGCAG
  AGAATCAGCTCCTTTGAAACCTTTGGCTCCTCTCAACTGCCTGACAAAGGAGCCCAGAGACTGAGCCTCC
  AGCCCTCCTCTGGGGAGGCAGCAAAACCTCTTGGGAAGCATGAGGAAGGACGGTTTTCTGGACTCTTGGG
  GCGAGGGGCTGCACCCACTCTTGTGCCCCAGCAGCCTGAGCAAGTACTGTCCTCGGGGTCCCCTGCAGCC
  TCCGAGGCCAGAGACCCAGGTGTGTCTGAGTCCCCTCCCCCAGGGCGGCAGCCCAATCAGAAAACTCTCC
  CCCCTGGCCCGGACCCGCTCCTAAGGCTGCTGTCAACACAGGCTGAGGAATCTCAAGGCCCAGTGCTCAA
  GATGCCTAGCCAGCGAGCACGGAGCTTCCCCCTGACCAGGTCCCAGTCCTGTGAGACGAAGCTACTTGAC
  GAAAAGACCAGCAAACTCTATTCTATCAGCAGCCAAGTGTCATCGGCTGTCATGAAATCCTTGCTGTGCC
  TTCCATCTTCTATCTCCTGTGCCCAGACTCCCTGCATCCCCAAGGAAGGGGCATCTCCAACATCATCATC
  CAACGAAGACTCAGCTGCAAATGGTTCTGCTGAAACATCTGCCTTGGACACAGGGTTCTCGCTCAACCTT
  TCAGAGCTGAGAGAATATACAGAGGGTCTCACGGAAGCCAAGGAAGACGATGATGGGGACCACAGTTCCC
  TTCAGTCTGGTCAGTCCGTTATCTCCCTGCTGAGCTCAGAAGAATTAAAAAAACTCATCGAGGAGGTGAA
  GGTTCTGGATGAAGCAACATTAAAGCAATTAGACGGCATCCATGTCACCATCTTACACAAGGAGGAAGGT
  GCTGGTCTTGGGTTCAGCTTGGCAGGAGGAGCAGATCTAGAAAACAAGGTGATTACGGTTCACAGAGTGT
  TTCCAAATGGGCTGGCCTCCCAGGAAGGGACTATTCAGAAGGGCAATGAGGTTCTTTCCATCAACGGCAA
  GTCTCTCAAGGGGACCACGCACCATGATGCCTTGGCCATCCTCCGCCAAGCTCGAGAGCCCAGGCAAGCT
  GTGATTGTCACAAGGAAGCTGACTCCAGAGGCCATGCCCGACCTCAACTCCTCCACTGACTCTGCAGCCT
  CAGCCTCTGCAGCCAGTGATGTTTCTGTAGAATCTACAGCAGAGGCCACAGTCTGCACGGTGACACTGGA
  GAAGATGTCGGCAGGGCTGGGCTTCAGCCTGGAAGGAGGGAAGGGCTCCCTACACGGAGACAAGCCTCTC
  ACCATTAACAGGATTTTCAAAGGAGCAGCCTCAGAACAAAGTGAGACAGTCCAGCCTGGAGATGAAATCT
  TGCAGCTGGGTGGCACTGCCATGCAGGGCCTCACACGGTTTGAAGCCTGGAACATCATCAAGGCACTGCC
  TGATGGACCTGTCACGATTGTCATCAGGAGAAAAAGCCTCCAGTCCAAGGAAACCACAGCTGCTGGAGAC
  TCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC220031 representing NM_004513
  Red=Cloning site Green=Tags(s)
  
  MDYSFDTTAEDPWVRISDCIKNLFSPIMSENHGHMPLQPNASLNEEEGTQGHPDGTPPKLDTANGTPKVY
  KSADSSTVKKGPPVAPKPAWFRQSLKGLRNRASDPRGLPDPALSTQPAPASREHLGSHIRASSSSSSIRQ
  RISSFETFGSSQLPDKGAQRLSLQPSSGEAAKPLGKHEEGRFSGLLGRGAAPTLVPQQPEQVLSSGSPAA
  SEARDPGVSESPPPGRQPNQKTLPPGPDPLLRLLSTQAEESQGPVLKMPSQRARSFPLTRSQSCETKLLD
  EKTSKLYSISSQVSSAVMKSLLCLPSSISCAQTPCIPKEGASPTSSSNEDSAANGSAETSALDTGFSLNL
  SELREYTEGLTEAKEDDDGDHSSLQSGQSVISLLSSEELKKLIEEVKVLDEATLKQLDGIHVTILHKEEG
  AGLGFSLAGGADLENKVITVHRVFPNGLASQEGTIQKGNEVLSINGKSLKGTTHHDALAILRQAREPRQA
  VIVTRKLTPEAMPDLNSSTDSAASASAASDVSVESTAEATVCTVTLEKMSAGLGFSLEGGKGSLHGDKPL
  TINRIFKGAASEQSETVQPGDEILQLGGTAMQGLTRFEAWNIIKALPDGPVTIVIRRKSLQSKETTAAGD
  S
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004513
• ORF Size: 1893 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004513.5
• RefSeq Size: 6193 bp
• RefSeq ORF: 1896 bp
• Locus ID: 3603
• UniProt ID: Q14005
• Cytogenetics: 15q25.1
• Domains: PDZ
• Protein Families: Druggable Genome, Secreted Protein
• MW: 66.5 kDa
• Gene Summary: The protein encoded by this gene is a pleiotropic cytokine that functions as a chemoattractant, a modulator of T cell activation, and an inhibitor of HIV replication. The signaling process of this cytokine is mediated by CD4. The product of this gene undergoes proteolytic processing, which is found to yield two functional proteins. The cytokine function is exclusively attributed to the secreted C-terminal peptide, while the N-terminal product may play a role in cell cycle control. Caspase 3 is reported to be involved in the proteolytic processing of this protein. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Feb 2010]