PCSK9 (NM_174936) Human Tagged ORF Clone

SKU
RC220000
PCSK9 (Myc-DDK-tagged)-Human proprotein convertase subtilisin/kexin type 9 (PCSK9)
  • TrueORF®
    TrueORF®

    Expression-ready ORF plasmid with C-terminal tag(s)

    Click here to learn more.

$589.00 MSRP $950.00 MSRP $950.00
In Stock*
Specifications
Product Data
Target Symbol PCSK9
Synonyms FH3; FHCL3; HCHOLA3; LDLCQ1; NARC-1; NARC1; PC9
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-RsrII Cloning Scheme for this gene Plasmid Map
ACCN NM_174936
ORF Size 2076 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_174936.4
RefSeq Size 3636 bp
RefSeq ORF 2079 bp
Locus ID 255738
UniProt ID Q8NBP7
Cytogenetics 1p32.3
Domains Peptidase_S8
Protein Families Secreted Protein
MW 71 kDa
Summary This gene encodes a member of the subtilisin-like proprotein convertase family, which includes proteases that process protein and peptide precursors trafficking through regulated or constitutive branches of the secretory pathway. The encoded protein undergoes an autocatalytic processing event with its prosegment in the ER and is constitutively secreted as an inactive protease into the extracellular matrix and trans-Golgi network. It is expressed in liver, intestine and kidney tissues and escorts specific receptors for lysosomal degradation. It plays a role in cholesterol and fatty acid metabolism. Mutations in this gene have been associated with autosomal dominant familial hypercholesterolemia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014]
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SKU Description Size Price
RC220000L1 Lenti ORF clone of Human proprotein convertase subtilisin/kexin type 9 (PCSK9), Myc-DDK-tagged 10 ug
$1,250.00
RC220000L2 Lenti ORF clone of Human proprotein convertase subtilisin/kexin type 9 (PCSK9), mGFP tagged 10 ug
$1,250.00
RC220000L3 Lenti ORF clone of Human proprotein convertase subtilisin/kexin type 9 (PCSK9), Myc-DDK-tagged 10 ug
$1,250.00
RC220000L4 Lenti ORF clone of Human proprotein convertase subtilisin/kexin type 9 (PCSK9), mGFP tagged 10 ug
$1,250.00
RG220000 PCSK9 (tGFP-tagged) - Human proprotein convertase subtilisin/kexin type 9 (PCSK9) 10 ug
$789.00 MSRP $1,150.00 MSRP $1,150.00
SC101299 PCSK9 (untagged)-Human proprotein convertase subtilisin/kexin type 9 (PCSK9) 10 ug
$952.00

Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.