CD30 (TNFRSF8) (NM_152942) Human Tagged ORF Clone
CAT#: RC219886
- TrueORF®
TNFRSF8 (Myc-DDK-tagged)-Human tumor necrosis factor receptor superfamily, member 8 (TNFRSF8), transcript variant 2
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_152942" in other vectors (4)
USD 198.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | CD30 |
Synonyms | CD30; D1S166E; Ki-1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC219886 representing NM_152942
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCCAGCCACTGATGGAGACCTGCCACAGCGTGGGGGCAGCCTACCTGGAGAGCCTGCCGCTGCAGG ATGCCAGCCCGGCCGGGGGCCCCTCGTCCCCCAGGGACCTTCCTGAGCCCCGGGTGTCCACGGAGCACAC CAATAACAAGATTGAGAAAATCTACATCATGAAGGCTGACACCGTGATCGTGGGGACCGTGAAGGCTGAG CTGCCGGAGGGCCGGGGCCTGGCGGGGCCAGCAGAGCCCGAGTTGGAGGAGGAGCTGGAGGCGGACCATA CCCCCCACTACCCCGAGCAGGAGACAGAACCGCCTCTGGGCAGCTGCAGCGATGTCATGCTCTCAGTGGA AGAGGAAGGGAAAGAAGACCCCTTGCCCACAGCTGCCTCTGGAAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC219886 representing NM_152942
Red=Cloning site Green=Tags(s) MSQPLMETCHSVGAAYLESLPLQDASPAGGPSSPRDLPEPRVSTEHTNNKIEKIYIMKADTVIVGTVKAE LPEGRGLAGPAEPELEEELEADHTPHYPEQETEPPLGSCSDVMLSVEEEGKEDPLPTAASGK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_152942 |
ORF Size | 396 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_152942.2, NP_694421.1 |
RefSeq Size | 2361 bp |
RefSeq ORF | 398 bp |
Locus ID | 943 |
Cytogenetics | 1p36.22 |
Protein Families | Druggable Genome, ES Cell Differentiation/IPS, Stem cell - Pluripotency, Transmembrane |
Protein Pathways | Cytokine-cytokine receptor interaction |
MW | 13.9 kDa |
Gene Summary | The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is expressed by activated, but not by resting, T and B cells. TRAF2 and TRAF5 can interact with this receptor, and mediate the signal transduction that leads to the activation of NF-kappaB. This receptor is a positive regulator of apoptosis, and also has been shown to limit the proliferative potential of autoreactive CD8 effector T cells and protect the body against autoimmunity. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC219886L3 | Lenti-ORF clone of TNFRSF8 (Myc-DDK-tagged)-Human tumor necrosis factor receptor superfamily, member 8 (TNFRSF8), transcript variant 2 |
USD 465.00 |
|
RC219886L4 | Lenti-ORF clone of TNFRSF8 (mGFP-tagged)-Human tumor necrosis factor receptor superfamily, member 8 (TNFRSF8), transcript variant 2 |
USD 465.00 |
|
RG219886 | TNFRSF8 (tGFP-tagged) - Human tumor necrosis factor receptor superfamily, member 8 (TNFRSF8), transcript variant 2 |
USD 365.00 |
|
SC306527 | TNFRSF8 (untagged)-Human tumor necrosis factor receptor superfamily, member 8 (TNFRSF8), transcript variant 2 |
USD 165.00 |
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