NEU2 (NM_005383) Human Tagged ORF Clone

CAT#: RC219858

NEU2 (Myc-DDK-tagged)-Human sialidase 2 (cytosolic sialidase) (NEU2)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY NEU2 (Cat# RC219858, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NEU2(Cat# TA502942). Positive lysates LY401652 (100ug) and LC401652 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY401652) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC219858 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified NEU2 protein (Cat# TP319858). The protein was produced from HEK293T cells transfected with NEU2 cDNA clone (Cat# RC219858) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NEU2
• Synonyms: SIAL2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC219858 representing NM_005383
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGTCCCTTCCTGTCCTGCAGAAGGAGAGCGTGTTCCAGTCGGGAGCCCATGCCTACAGAATCCCTG
  CCCTGCTCTACCTGCCTGGGCAGCAGTCCCTGCTGGCCTTCGCGGAACAGCGGGCAAGCAAGAAGGATGA
  GCACGCAGAGCTGATTGTCCTGCGCAGAGGAGACTACGACGCACCCACCCACCAGGTTCAGTGGCAAGCT
  CAGGAGGTGGTGGCCCAGGCCCGGCTGGATGGCCACCGGTCCATGAACCCATGCCCCTTGTATGACGCGC
  AGACGGGGACCCTCTTCCTCTTCTTCATTGCCATCCCTGGGCAAGTCACGGAGCAACAGCAGCTGCAGAC
  CAGGGCCAATGTGACGCGGCTGTGCCAAGTCACCAGCACTGACCACGGGAGGACCTGGAGCTCCCCCAGA
  GACCTCACTGATGCGGCCATCGGCCCAGCCTACCGGGAGTGGTCCACCTTTGCAGTGGGCCCGGGGCATT
  GTTTGCAGCTTAACGACAGGGCCCGGAGCCTGGTGGTGCCCGCCTACGCCTACCGGAAACTTCACCCCAT
  CCAAAGGCCGATCCCCTCTGCCTTCTGCTTCCTCAGCCATGACCATGGGCGCACGTGGGCGCGAGGGCAC
  TTTGTGGCCCAGGACACCCTGGAGTGCCAGGTGGCCGAAGTCGAGACTGGGGAGCAGAGGGTGGTGACCC
  TCAACGCGAGAAGCCACCTCCGAGCCAGGGTCCAGGCCCAGAGCACCAATGACGGGCTTGATTTCCAGGA
  GTCTCAGCTGGTGAAGAAGCTGGTGGAGCCGCCGCCCCAGGGCTGCCAGGGGAGCGTCATCAGCTTCCCC
  AGCCCCCGCTCGGGGCCTGGCTCCCCAGCCCAGTGGCTGCTCTACACTCACCCCACACACTCCTGGCAGA
  GGGCCGACCTGGGTGCCTACCTCAACCCGCGACCTCCAGCCCCTGAGGCCTGGTCAGAGCCGGTACTGCT
  GGCCAAGGGCAGCTGTGCCTACTCAGACCTCCAGAGCATGGGCACCGGCCCTGATGGGTCCCCCTTGTTT
  GGGTGTCTGTACGAAGCCAATGATTACGAGGAGATTGTCTTTCTCATGTTCACCCTGAAGCAAGCCTTCC
  CAGCTGAGTACCTGCCTCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC219858 representing NM_005383
  Red=Cloning site Green=Tags(s)
  
  MASLPVLQKESVFQSGAHAYRIPALLYLPGQQSLLAFAEQRASKKDEHAELIVLRRGDYDAPTHQVQWQA
  QEVVAQARLDGHRSMNPCPLYDAQTGTLFLFFIAIPGQVTEQQQLQTRANVTRLCQVTSTDHGRTWSSPR
  DLTDAAIGPAYREWSTFAVGPGHCLQLNDRARSLVVPAYAYRKLHPIQRPIPSAFCFLSHDHGRTWARGH
  FVAQDTLECQVAEVETGEQRVVTLNARSHLRARVQAQSTNDGLDFQESQLVKKLVEPPPQGCQGSVISFP
  SPRSGPGSPAQWLLYTHPTHSWQRADLGAYLNPRPPAPEAWSEPVLLAKGSCAYSDLQSMGTGPDGSPLF
  GCLYEANDYEEIVFLMFTLKQAFPAEYLPQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005383
• ORF Size: 1140 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005383.1, NP_005374.1
• RefSeq Size: 1143 bp
• RefSeq ORF: 1143 bp
• Locus ID: 4759
• UniProt ID: Q9Y3R4
• Cytogenetics: 2q37.1
• Protein Pathways: Other glycan degradation, Sphingolipid metabolism
• MW: 42.1 kDa
• Gene Summary: This gene belongs to a family of glycohydrolytic enzymes which remove sialic acid residues from glycoproteins and glycolipids. Expression studies in COS7 cells confirmed that this gene encodes a functional sialidase. Its cytosolic localization was demonstrated by cell fractionation experiments. [provided by RefSeq, Jul 2008]