KLHL4 (NM_019117) Human Tagged ORF Clone

CAT#: RC219838

KLHL4 (Myc-DDK-tagged)-Human kelch-like 4 (Drosophila) (KLHL4), transcript variant 1

Product Images

Circular map for RC219838

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: KLHL4
• Synonyms: DKELCHL; KHL4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC219838 representing NM_019117
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCAGTGTCTGGCAAGAAAGAGTTTGATGTGAAACAGATCCTAAGGCTACGCTGGAGGTGGTTTAGTC
  ATCCTTTTCAAGGTTCCACCAACACTGGAAGCTGTCTTCAGCAGGAAGGATATGAGCATAGAGGGACCCC
  GGTTCAGGGCAGGTTGAAGAGCCACTCTCGGGACAGAAACGGACTGAAGAAAAGCAACAGTCCTGTCCAC
  CACAATATACTGGCACCAGTGCCAGGACCGGCCCCTGCCCATCAGAGAGCCGTTCAGAATTTGCAGCAAC
  ATAATCTGATAGTACATTTTCAAGCAAATGAAGATACTCCTAAATCAGTTCCAGAGAAGAATTTATTCAA
  AGAAGCTTGTGAGAAACGCGCACAAGATTTGGAGATGATGGCTGATGACAATATAGAAGATTCTACAGCA
  AGATTAGATACACAACACTCTGAAGACATGAATGCCACCAGATCTGAAGAGCAGTTCCATGTTATAAACC
  ACGCAGAGCAAACTCTTCGTAAAATGGAGAACTACTTGAAAGAGAAACAACTATGTGATGTGCTACTGAT
  TGCAGGACACCTCCGCATCCCAGCCCATAGGTTGGTTCTCAGCGCAGTGTCTGATTATTTTGCTGCAATG
  TTTACTAATGATGTGCTTGAAGCCAAACAAGAAGAGGTCAGGATGGAAGGAGTAGATCCAAATGCACTAA
  ATTCCTTGGTGCAGTATGCTTACACAGGAGTCCTGCAATTGAAAGAAGATACCATTGAAAGTTTGCTGGC
  TGCAGCTTGTCTTCTGCAGCTGACTCAGGTCATTGATGTTTGCTCCAATTTTCTCATAAAGCAGCTCCAT
  CCTTCAAACTGCTTAGGGATTCGATCATTTGGAGATGCCCAAGGCTGTACAGAACTTCTGAACGTGGCAC
  ACAAATACACTATGGAACACTTCATTGAGGTAATAAAAAACCAAGAATTCCTCCTGCTTCCAGCTAATGA
  AATTTCAAAACTTCTGTGCAGTGATGACATTAATGTGCCTGATGAAGAGACCATTTTTCATGCTCTAATG
  CAGTGGGTGGGGCATGATGTGCAGAATAGGCAAGGAGAACTGGGGATGCTGCTTTCTTACATCAGACTGC
  CATTACTCCCACCACAGTTACTGGCAGATCTTGAAACCAGTTCCATGTTTACTGGTGATCTTGAGTGTCA
  GAAGCTCCTGATGGAAGCTATGAAGTATCATCTTTTGCCTGAGAGAAGATCCATGATGCAAAGCCCTCGG
  ACAAAGCCTAGAAAATCAACTGTGGGGGCACTTTATGCTGTAGGAGGCATGGATGCTATGAAAGGTACTA
  CTACTATTGAAAAATATGACCTCAGGACCAACAGTTGGCTACATATTGGCACCATGAATGGCCGTAGGCT
  TCAATTTGGAGTCGCAGTTATTGATAATAAGCTCTATGTCGTGGGAGGAAGAGACGGTTTAAAAACTTTG
  AATACAGTGGAATGTTTTAATCCAGTTGGCAAAATCTGGACTGTGATGCCTCCCATGTCAACACATCGGC
  ACGGCTTAGGTGTAGCCACTCTTGAAGGACCAATGTATGCTGTAGGTGGTCATGATGGATGGAGCTATCT
  AAATACTGTAGAAAGATGGGACCCTGAGGGACGACAGTGGAATTACGTAGCCAGTATGTCAACTCCTAGA
  AGCACAGTTGGTGTTGTTGCATTAAACAACAAATTATATGCTATTGGTGGACGTGATGGAAGTTCCTGCC
  TCAAATCAATGGAATACTTTGACCCACACACTAACAAGTGGAGTTTGTGTGCTCCAATGTCCAAAAGACG
  TGGAGGTGTGGGAGTTGCCACATACAATGGATTCTTATATGTTGTAGGGGGGCATGATGCCCCTGCTTCC
  AACCATTGCTCCAGGCTTTCTGACTGTGTGGAACGGTATGATCCAAAAGGTGATTCATGGTCAACTGTGG
  CACCTCTGAGTGTTCCTCGAGATGCTGTTGCTGTGTGCCCTCTTGGAGACAAACTCTACGTGGTTGGAGG
  ATATGACGGACATACTTATTTGAACACAGTTGAGTCATATGATGCACAGAGAAATGAATGGAAAGAGGAA
  GTTCCTGTTAACATTGGAAGAGCTGGTGCATGTGTTGTAGTGGTGAAGCTACCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC219838 representing NM_019117
  Red=Cloning site Green=Tags(s)
  
  MSVSGKKEFDVKQILRLRWRWFSHPFQGSTNTGSCLQQEGYEHRGTPVQGRLKSHSRDRNGLKKSNSPVH
  HNILAPVPGPAPAHQRAVQNLQQHNLIVHFQANEDTPKSVPEKNLFKEACEKRAQDLEMMADDNIEDSTA
  RLDTQHSEDMNATRSEEQFHVINHAEQTLRKMENYLKEKQLCDVLLIAGHLRIPAHRLVLSAVSDYFAAM
  FTNDVLEAKQEEVRMEGVDPNALNSLVQYAYTGVLQLKEDTIESLLAAACLLQLTQVIDVCSNFLIKQLH
  PSNCLGIRSFGDAQGCTELLNVAHKYTMEHFIEVIKNQEFLLLPANEISKLLCSDDINVPDEETIFHALM
  QWVGHDVQNRQGELGMLLSYIRLPLLPPQLLADLETSSMFTGDLECQKLLMEAMKYHLLPERRSMMQSPR
  TKPRKSTVGALYAVGGMDAMKGTTTIEKYDLRTNSWLHIGTMNGRRLQFGVAVIDNKLYVVGGRDGLKTL
  NTVECFNPVGKIWTVMPPMSTHRHGLGVATLEGPMYAVGGHDGWSYLNTVERWDPEGRQWNYVASMSTPR
  STVGVVALNNKLYAIGGRDGSSCLKSMEYFDPHTNKWSLCAPMSKRRGGVGVATYNGFLYVVGGHDAPAS
  NHCSRLSDCVERYDPKGDSWSTVAPLSVPRDAVAVCPLGDKLYVVGGYDGHTYLNTVESYDAQRNEWKEE
  VPVNIGRAGACVVVVKLP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_019117
• ORF Size: 2154 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_019117.5
• RefSeq Size: 5880 bp
• RefSeq ORF: 2157 bp
• Locus ID: 56062
• UniProt ID: Q9C0H6
• Cytogenetics: Xq21.31
• Domains: BTB, Kelch
• MW: 80.2 kDa
• Gene Summary: This gene encodes a member of the kelch family of proteins, which are characterized by kelch repeat motifs and a POZ/BTB protein-binding domain. It is thought that kelch repeats are actin binding domains. However, the specific function of this protein has not been determined. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]