NDRG2 (NM_201535) Human Tagged ORF Clone

CAT#: RC219812

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NDRG2 (Myc-DDK-tagged)-Human NDRG family member 2 (NDRG2), transcript variant 1



Reconstitution Protocol

USD 686.00

USD 1,029.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol NDRG2
Synonyms SYLD
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC219812 representing NM_201535
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGAGCTGCAGGAGGTGCAGATCACAGAGGAGAAGCCACTGTTGCCAGGACAGACGCCTGAGGCGG
CCAAGGAGGCTGAGTTAGCTGCCCGAATCCTCCTGGACCAGGGACAGACTCACTCTGTGGAGACACCATA
CGGCTCTGTCACTTTCACTGTCTATGGCACCCCCAAACCCAAACGCCCAGCGATCCTTACCTACCACGAT
GTGGGACTCAACTATAAATCTTGCTTCCAGCCACTGTTTCAGTTCGAGGACATGCAGGAAATCATTCAGA
ACTTTGTGCGGGTTCATGTGGATGCCCCTGGAATGGAAGAGGGAGCCCCTGTGTTCCCTTTGGGATATCA
GTACCCATCTCTGGACGAGCTTGCAGACATGATCCCTTGCGTCCTGCAGTACCTAAATTTCTCTACAATA
ATTGGAGTTGGTGTTGGAGCTGGAGCCTACATCCTGGCGAGATATGCTCTTAACCACCCGGACACTGTTG
AAGGTCTTGTCCTCATCAACATTGATCCCAATGCCAAGGGTTGGATGGATTGGGCAGCCCACAAGCTAAC
AGGCCTCACCTCTTCCATTCCGGAGATGATCCTTGGACATCTTTTCAGCCAGGAAGAGCTCTCTGGAAAT
TCTGAGTTGATACAAAAGTACAGAAATATCATTACACATGCACCCAACCTGGATAACATTGAATTGTACT
GGAACAGCTACAACAACCGCCGAGACCTGAACTTTGAGCGTGGAGGTGATATCACCCTCAGGTGTCCTGT
GATGCTGGTGGTAGGAGACCAAGCACCTCATGAAGATGCAGTGGTGGAATGTAACTCAAAACTGGACCCC
ACCCGGACCTCGTTCCTCAAGATGGCTGACTCCGGAGGTCAGCCCCAGCTGACTCAGCCAGGCAAGCTGA
CCGAGGCCTTCAAGTACTTCCTGCAAGGCATGGGCTACATGGCCTCATCCTGCATGACTCGCCTGTCCCG
GTCTCGTACAGCCTCTCTGACCAGTGCAGCATCCGTTGATGGCAACAGGTCCCGCTCTCGCACCCTGTCC
CAGAGCAGCGAGTCTGGAACTCTTTCTTCGGGGCCCCCGGGGCACACCATGGAGGTCTCCTGT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC219812 representing NM_201535
Red=Cloning site Green=Tags(s)

MAELQEVQITEEKPLLPGQTPEAAKEAELAARILLDQGQTHSVETPYGSVTFTVYGTPKPKRPAILTYHD
VGLNYKSCFQPLFQFEDMQEIIQNFVRVHVDAPGMEEGAPVFPLGYQYPSLDELADMIPCVLQYLNFSTI
IGVGVGAGAYILARYALNHPDTVEGLVLINIDPNAKGWMDWAAHKLTGLTSSIPEMILGHLFSQEELSGN
SELIQKYRNIITHAPNLDNIELYWNSYNNRRDLNFERGGDITLRCPVMLVVGDQAPHEDAVVECNSKLDP
TRTSFLKMADSGGQPQLTQPGKLTEAFKYFLQGMGYMASSCMTRLSRSRTASLTSAASVDGNRSRSRTLS
QSSESGTLSSGPPGHTMEVSC

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_201535
ORF Size 1113 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_201535.1, NP_963293.1
RefSeq Size 2142 bp
RefSeq ORF 1116 bp
Locus ID 57447
UniProt ID Q9UN36
Cytogenetics 14q11.2
MW 40.6 kDa
Gene Summary This gene is a member of the N-myc downregulated gene family which belongs to the alpha/beta hydrolase superfamily. The protein encoded by this gene is a cytoplasmic protein that may play a role in neurite outgrowth. This gene may be involved in glioblastoma carcinogenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2017]
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