LASS6 (CERS6) (NM_203463) Human Tagged ORF Clone

CAT#: RC219555

CERS6 (Myc-DDK-tagged)-Human LAG1 homolog, ceramide synthase 6 (LASS6)

Product Images

Circular map for RC219555

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  Western blot validation of overexpression lysate (Cat# LY404278) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC219555 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified CERS6 protein (Cat# TP319555). The protein was produced from HEK293T cells transfected with CERS6 cDNA clone (Cat# RC219555) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: LASS6
• Synonyms: CERS5; LASS6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC219555 representing NM_203463
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGGGATCTTAGCCTGGTTCTGGAACGAGAGGTTTTGGCTCCCGCACAATGTCACCTGGGCGGACC
  TGAAGAACACGGAGGAGGCCACCTTCCCGCAGGCTGAGGACCTCTATCTCGCTTTTCCCCTGGCCTTCTG
  TATCTTCATGGTGCGGCTCATCTTCGAGAGATTTGTAGCCAAACCGTGCGCCATAGCCCTCAACATTCAG
  GCCAATGGACCACAAATTGCTCCGCCCAATGCCATTCTGGAAAAGGTCTTCACTGCAATTACAAAGCATC
  CTGATGAAAAGAGATTGGAAGGCCTCTCCAAGCAACTGGACTGGGATGTTCGAAGCATTCAGCGCTGGTT
  TCGACAAAGACGCAATCAGGAGAAGCCAAGCACGCTGACGAGGTTCTGTGAGAGCATGTGGAGATTTTCA
  TTTTACCTTTATGTATTTACCTACGGAGTCAGATTCCTGAAAAAGACCCCCTGGTTGTGGAATACGAGGC
  ATTGCTGGTACAACTACCCCTATCAGCCACTCACAACTGACCTTCACTACTATTACATCCTGGAGCTGTC
  GTTTTATTGGTCTTTGATGTTTTCTCAGTTCACTGATATCAAAAGAAAGGACTTTGGCATTATGTTCCTG
  CACCACCTTGTATCTATTTTCTTGATTACCTTTTCATATGTCAACAATATGGCCCGAGTAGGAACGCTGG
  TCCTTTGTCTTCATGATTCAGCTGATGCTCTTCTGGAGGCTGCCAAAATGGCAAATTATGCCAAGTTTCA
  GAAAATGTGTGATCTCCTGTTTGTTATGTTTGCCGTGGTTTTTATCACCACACGACTGGGTATATTTCCT
  CTCTGGGTGTTAAATACCACATTATTTGAAAGCTGGGAGATCGTTGGACCTTACCCTTCCTGGTGGGTTT
  TTAACCTACTGCTATTGCTAGTACAAGGGTTGAACTGCTTCTGGTCTTACTTGATTGTGAAAATAGCTTG
  CAAAGCTGTTTCAAGAGGCAAGGTGTCCAAGGATGATCGAAGTGATATTGAGTCTAGCTCAGATGAGGAG
  GACTCAGAACCTCCGGGAAAGAATCCCCACACTGCGACAACCACCAATGGGACCAGTGGTACCAACGGGT
  ATCTCCTGACTGGCTCCTGCTCCATGGATGAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC219555 representing NM_203463
  Red=Cloning site Green=Tags(s)
  
  MAGILAWFWNERFWLPHNVTWADLKNTEEATFPQAEDLYLAFPLAFCIFMVRLIFERFVAKPCAIALNIQ
  ANGPQIAPPNAILEKVFTAITKHPDEKRLEGLSKQLDWDVRSIQRWFRQRRNQEKPSTLTRFCESMWRFS
  FYLYVFTYGVRFLKKTPWLWNTRHCWYNYPYQPLTTDLHYYYILELSFYWSLMFSQFTDIKRKDFGIMFL
  HHLVSIFLITFSYVNNMARVGTLVLCLHDSADALLEAAKMANYAKFQKMCDLLFVMFAVVFITTRLGIFP
  LWVLNTTLFESWEIVGPYPSWWVFNLLLLLVQGLNCFWSYLIVKIACKAVSRGKVSKDDRSDIESSSDEE
  DSEPPGKNPHTATTTNGTSGTNGYLLTGSCSMDD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_203463
• ORF Size: 1152 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.

Reference Data

• RefSeq: NM_203463.3
• RefSeq Size: 6259 bp
• RefSeq ORF: 1155 bp
• Locus ID: 253782
• UniProt ID: Q6ZMG9
• Cytogenetics: 2q24.3
• Protein Families: Druggable Genome, Transmembrane
• MW: 44.7 kDa
• Gene Summary: May be involved in sphingolipid synthesis or its regulation.[UniProtKB/Swiss-Prot Function]