GABA B Receptor 2 (GABBR2) (NM_005458) Human Tagged ORF Clone

CAT#: RC219404

GABBR2 (Myc-DDK-tagged)-Human gamma-aminobutyric acid (GABA) B receptor, 2 (GABBR2)

Product Images

Circular map for RC219404

• 

  Western blot validation of overexpression lysate (Cat# LY417297) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC219404 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GABA B Receptor 2
• Synonyms: DEE59; EIEE59; GABABR2; GPR51; GPRC3B; HG20; HRIHFB2099; NDPLHS
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC219404 representing NM_005458
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTTCCCCGCGGAGCTCCGGGCAGCCCGGGCCGCCGCCGCCGCCGCCACCGCCGCCCGCGCGCCTGC
  TACTGCTACTGCTGCTGCCGCTGCTGCTGCCTCTGGCGCCCGGGGCCTGGGGCTGGGCGCGGGGCGCCCC
  CCGGCCGCCGCCCAGCAGCCCGCCGCTCTCCATCATGGGCCTCATGCCGCTCACCAAGGAGGTGGCCAAG
  GGCAGCATCGGGCGCGGTGTGCTCCCCGCCGTGGAACTGGCCATCGAGCAGATCCGCAACGAGTCACTCC
  TGCGCCCCTACTTCCTCGACCTGCGGCTCTATGACACGGAGTGCGACAACGCAAAAGGGTTGAAAGCCTT
  CTACGATGCAATAAAATACGGGCCGAACCACTTGATGGTGTTTGGAGGCGTCTGTCCATCCGTCACATCC
  ATCATTGCAGAGTCCCTCCAAGGCTGGAATCTGGTGCAGCTTTCTTTTGCTGCAACCACGCCTGTTCTAG
  CCGATAAGAAAAAATACCCTTATTTCTTTCGGACCGTCCCATCAGACAATGCGGTGAATCCAGCCATTCT
  GAAGTTGCTCAAGCACTACCAGTGGAAGCGCGTGGGCACGCTGACGCAAGACGTTCAGAGGTTCTCTGAG
  GTGCGGAATGACCTGACTGGAGTTCTGTATGGCGAGGACATTGAGATTTCAGACACCGAGAGCTTCTCCA
  ACGATCCCTGTACCAGTGTCAAAAAGCTGAAGGGGAATGATGTGCGGATCATCCTTGGCCAGTTTGACCA
  GAATATGGCAGCAAAAGTGTTCTGTTGTGCATACGAGGAGAACATGTATGGTAGTAAATATCAGTGGATC
  ATTCCGGGCTGGTACGAGCCTTCTTGGTGGGAGCAGGTGCACACGGAAGCCAACTCATCCCGCTGCCTCC
  GGAAGAATCTGCTTGCTGCCATGGAGGGCTACATTGGCGTGGATTTCGAGCCCCTGAGCTCCAAGCAGAT
  CAAGACCATCTCAGGAAAGACTCCACAGCAGTATGAGAGAGAGTACAACAACAAGCGGTCAGGCGTGGGG
  CCCAGCAAGTTCCACGGGTACGCCTACGATGGCATCTGGGTCATCGCCAAGACACTGCAGAGGGCCATGG
  AGACACTGCATGCCAGCAGCCGGCACCAGCGGATCCAGGACTTCAACTACACGGACCACACGCTGGGCAG
  GATCATCCTCAATGCCATGAACGAGACCAACTTCTTCGGGGTCACGGGTCAAGTTGTATTCCGGAATGGG
  GAGAGAATGGGGACCATTAAATTTACTCAATTTCAAGACAGCAGGGAGGTGAAGGTGGGAGAGTACAACG
  CTGTGGCCGACACACTGGAGATCATCAATGACACCATCAGGTTCCAAGGATCCGAACCACCAAAAGACAA
  GACCATCATCCTGGAGCAGCTGCGGAAGATCTCCCTACCTCTCTACAGCATCCTCTCTGCCCTCACCATC
  CTCGGGATGATCATGGCCAGTGCTTTTCTCTTCTTCAACATCAAGAACCGGAATCAGAAGCTCATAAAGA
  TGTCGAGTCCATACATGAACAACCTTATCATCCTTGGAGGGATGCTCTCCTATGCTTCCATATTTCTCTT
  TGGCCTTGATGGATCCTTTGTCTCTGAAAAGACCTTTGAAACACTTTGCACCGTCAGGACCTGGATTCTC
  ACCGTGGGCTACACGACCGCTTTTGGGGCCATGTTTGCAAAGACCTGGAGAGTCCACGCCATCTTCAAAA
  ATGTGAAAATGAAGAAGAAGATCATCAAGGACCAGAAACTGCTTGTGATCGTGGGGGGCATGCTGCTGAT
  CGACCTGTGTATCCTGATCTGCTGGCAGGCTGTGGACCCCCTGCGAAGGACAGTGGAGAAGTACAGCATG
  GAGCCGGACCCAGCAGGACGGGATATCTCCATCCGCCCTCTCCTGGAGCACTGTGAGAACACCCATATGA
  CCATCTGGCTTGGCATCGTCTATGCCTACAAGGGACTTCTCATGTTGTTCGGTTGTTTCTTAGCTTGGGA
  GACCCGCAACGTCAGCATCCCCGCACTCAACGACAGCAAGTACATCGGGATGAGTGTCTACAACGTGGGG
  ATCATGTGCATCATCGGGGCCGCTGTCTCCTTCCTGACCCGGGACCAGCCCAATGTGCAGTTCTGCATCG
  TGGCTCTGGTCATCATCTTCTGCAGCACCATCACCCTCTGCCTGGTATTCGTGCCGAAGCTCATCACCCT
  GAGAACAAACCCAGATGCAGCAACGCAGAACAGGCGATTCCAGTTCACTCAGAATCAGAAGAAAGAAGAT
  TCTAAAACGTCCACCTCGGTCACCAGTGTGAACCAAGCCAGCACATCCCGCCTGGAGGGCCTACAGTCAG
  AAAACCATCACCTGCGAATGAAGATCACAGAGCTGGATAAAGACTTGGAAGAGGTCACCATGCAGCTGCA
  GGACACACCAGAAAAGACCACCTACATTAAACAGAACCACTACCAAGAGCTCAATGACATCCTCAACCTG
  GGAAACTTCACTGAGAGCACAGATGGAGGAAAGGCCATTTTAAAAAATCACCTCGATCAAAATCCCCAGC
  TACAGTGGAACACAACAGAGCCCTCTCGAACATGCAAAGATCCTATAGAAGATATAAACTCTCCAGAACA
  CATCCAGCGTCGGCTGTCCCTCCAGCTCCCCATCCTCCACCACGCCTACCTCCCATCCATCGGAGGCGTG
  GACGCCAGCTGTGTCAGCCCCTGCGTCAGCCCCACCGCCAGCCCCCGCCACAGACATGTGCCACCCTCCT
  TCCGAGTCATGGTCTCGGGCCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC219404 representing NM_005458
  Red=Cloning site Green=Tags(s)
  
  MASPRSSGQPGPPPPPPPPPARLLLLLLLPLLLPLAPGAWGWARGAPRPPPSSPPLSIMGLMPLTKEVAK
  GSIGRGVLPAVELAIEQIRNESLLRPYFLDLRLYDTECDNAKGLKAFYDAIKYGPNHLMVFGGVCPSVTS
  IIAESLQGWNLVQLSFAATTPVLADKKKYPYFFRTVPSDNAVNPAILKLLKHYQWKRVGTLTQDVQRFSE
  VRNDLTGVLYGEDIEISDTESFSNDPCTSVKKLKGNDVRIILGQFDQNMAAKVFCCAYEENMYGSKYQWI
  IPGWYEPSWWEQVHTEANSSRCLRKNLLAAMEGYIGVDFEPLSSKQIKTISGKTPQQYEREYNNKRSGVG
  PSKFHGYAYDGIWVIAKTLQRAMETLHASSRHQRIQDFNYTDHTLGRIILNAMNETNFFGVTGQVVFRNG
  ERMGTIKFTQFQDSREVKVGEYNAVADTLEIINDTIRFQGSEPPKDKTIILEQLRKISLPLYSILSALTI
  LGMIMASAFLFFNIKNRNQKLIKMSSPYMNNLIILGGMLSYASIFLFGLDGSFVSEKTFETLCTVRTWIL
  TVGYTTAFGAMFAKTWRVHAIFKNVKMKKKIIKDQKLLVIVGGMLLIDLCILICWQAVDPLRRTVEKYSM
  EPDPAGRDISIRPLLEHCENTHMTIWLGIVYAYKGLLMLFGCFLAWETRNVSIPALNDSKYIGMSVYNVG
  IMCIIGAAVSFLTRDQPNVQFCIVALVIIFCSTITLCLVFVPKLITLRTNPDAATQNRRFQFTQNQKKED
  SKTSTSVTSVNQASTSRLEGLQSENHHLRMKITELDKDLEEVTMQLQDTPEKTTYIKQNHYQELNDILNL
  GNFTESTDGGKAILKNHLDQNPQLQWNTTEPSRTCKDPIEDINSPEHIQRRLSLQLPILHHAYLPSIGGV
  DASCVSPCVSPTASPRHRHVPPSFRVMVSGL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005458
• ORF Size: 2823 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005458.8
• RefSeq Size: 5786 bp
• RefSeq ORF: 2826 bp
• Locus ID: 9568
• UniProt ID: O75899
• Cytogenetics: 9q22.33
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Neuroactive ligand-receptor interaction
• MW: 105.8 kDa
• Gene Summary: The multi-pass membrane protein encoded by this gene belongs to the G-protein coupled receptor 3 family and GABA-B receptor subfamily. The GABA-B receptors inhibit neuronal activity through G protein-coupled second-messenger systems, which regulate the release of neurotransmitters, and the activity of ion channels and adenylyl cyclase. This receptor subunit forms an active heterodimeric complex with GABA-B receptor subunit 1, neither of which is effective on its own. Allelic variants of this gene have been associated with nicotine dependence.[provided by RefSeq, Jan 2010]