MAP3K19 (NM_001018046) Human Tagged ORF Clone

CAT#: RC219370

MAP3K19 (Myc-DDK-tagged)-Human YSK4 Sps1/Ste20-related kinase homolog (S. cerevisiae) (YSK4), transcript variant 2

Product Images

Circular map for RC219370

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  Western blot validation of overexpression lysate (Cat# LY422703) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC219370 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified MAP3K19 protein (Cat# TP319370). The protein was produced from HEK293T cells transfected with MAP3K19 cDNA clone (Cat# RC219370) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MAP3K19
• Synonyms: RCK; YSK4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC219370 representing NM_001018046
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTTCTATGCCAAAACCAGAAAGACATGCTGAGTCATTGCTTGACATTTGTCATGATACAAACTCTT
  CTCCAACTGATTTGATGACAGTTACCAAAAATCAAAACATCATCTTGCAAAGCATCAGCAGAAGTGAGGA
  GTTCGACCAAGATGGTGACTGCAGTCATTCCACACTGGTTAATGAAGAAGAAGATCCCAGTGGTGGTAGA
  CAGGACTGGCAACCCAGGACAGAAGGTGTTGAGATCACTGTAACTTTTCCAAGAGATGTCAGTCCTCCCC
  AAGAAATGAGCCAAGAAGACTTAAAAGAAAAGAATCTGATAAACTCATCGCTTCAAGAATGGGCACAAGC
  ACATGCAGTTTCTCATCCAAATGAAATAGAAACGGTGGAGCTCAGGAAAAAGAAGCTGACCATGCGGCCC
  TTAGTTTTGCAAAAAGAGGAAAGTTCCAGGGAGCTCTGCAATGTGAACTTGGGCTTTTTGCTACCAAGAT
  CTTGTTTAGAACTGAACATTTCCAAGTCTGTAACCAGAGAAGATGCTCCTCATTTTCTGAAGGAGCAGCA
  AAGAAAATCTGAAGAGTTTTCGACCTCTCATATGAAGTACAGTGGCCGAAGCATCAAGAGGCATAGTAGT
  GGGCTCAGGATATATGACAGGGAGGAGAAATTTCTCATCTCAAATGAAAAGAAGATATTTTCTGAAAATA
  GTTTAAAGTCTGAAGAACCTATCCTATGGACCAAGGGTGAGATTCTTGGAAAGGGAGCCTACGGCACAGT
  ATACTGTGGTCTCACTAGTCAAGGACAGCTAATAGCTGTAAAACAGGTGGCTTTGGATACCTCTAATAAA
  TTAGCTGCTGAAAAGGAATACCGGAAACTACAGGAAGAAGTAGATTTGCTCAAAGCACTGAAACATGTCA
  ACATTGTGGCCTATTTGGGGACATGCTTGCAAGAGAACACTGTGAGCATTTTCATGGAGTTTGTTCCTGG
  TGGCTCAATCTCTAGTATTATAAACCGTTTTGGGCCATTGCCTGAGATGGTGTTCTGTAAATATACGAAA
  CAAATACTTCAAGGTGTTGCTTATCTCCATGAGAACTGTGTGGTACATCGCGATATCAAAGGAAATAATG
  TTATGCTCATGCCAACTGGAATAATAAAGCTGATTGACTTTGGCTGTGCCAGGCGTTTGGCCTGGGCAGG
  TTTAAATGGCACCCACAGTGACATGCTTAAGTCCATGCATGGGACTCCATATTGGATGGCCCCAGAAGTC
  ATCAATGAGTCTGGCTATGGACGGAAATCAGATATCTGGAGCATTGGTTGTACTGTGTTTGAGATGGCTA
  CAGGGAAGCCTCCACTGGCTTCCATGGACAGGATGGCCGCCATGTTTTACATCGGAGCACACCGAGGGCT
  GATGCCTCCTTTACCAGACCACTTCTCAGAAAATGCAGCAGACTTTGTGCGCATGTGCCTGACCAGGGAC
  CAGCATGAGCGACCTTCTGCTCTCCAGCTCCTGAAGCACTCCTTCTTGGAGAGAAGTCAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC219370 representing NM_001018046
  Red=Cloning site Green=Tags(s)
  
  MSSMPKPERHAESLLDICHDTNSSPTDLMTVTKNQNIILQSISRSEEFDQDGDCSHSTLVNEEEDPSGGR
  QDWQPRTEGVEITVTFPRDVSPPQEMSQEDLKEKNLINSSLQEWAQAHAVSHPNEIETVELRKKKLTMRP
  LVLQKEESSRELCNVNLGFLLPRSCLELNISKSVTREDAPHFLKEQQRKSEEFSTSHMKYSGRSIKRHSS
  GLRIYDREEKFLISNEKKIFSENSLKSEEPILWTKGEILGKGAYGTVYCGLTSQGQLIAVKQVALDTSNK
  LAAEKEYRKLQEEVDLLKALKHVNIVAYLGTCLQENTVSIFMEFVPGGSISSIINRFGPLPEMVFCKYTK
  QILQGVAYLHENCVVHRDIKGNNVMLMPTGIIKLIDFGCARRLAWAGLNGTHSDMLKSMHGTPYWMAPEV
  INESGYGRKSDIWSIGCTVFEMATGKPPLASMDRMAAMFYIGAHRGLMPPLPDHFSENAADFVRMCLTRD
  QHERPSALQLLKHSFLERSH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001018046
• ORF Size: 1530 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001018046.3
• RefSeq Size: 1711 bp
• RefSeq ORF: 1533 bp
• Locus ID: 80122
• UniProt ID: Q56UN5
• Cytogenetics: 2q21.3
• Protein Families: Druggable Genome, Protein Kinase
• MW: 57.5 kDa