MAP3K19 (NM_001018046) Human Tagged ORF Clone

CAT#: RC219370

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MAP3K19 (Myc-DDK-tagged)-Human YSK4 Sps1/Ste20-related kinase homolog (S. cerevisiae) (YSK4), transcript variant 2



  "NM_001018046" in other vectors (6)

Reconstitution Protocol

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 544.00


Availability*
In Stock

Size
    • 10 ug


Product images

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol MAP3K19
Synonyms RCK; YSK4
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC219370 representing NM_001018046
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGTTCTATGCCAAAACCAGAAAGACATGCTGAGTCATTGCTTGACATTTGTCATGATACAAACTCTT
CTCCAACTGATTTGATGACAGTTACCAAAAATCAAAACATCATCTTGCAAAGCATCAGCAGAAGTGAGGA
GTTCGACCAAGATGGTGACTGCAGTCATTCCACACTGGTTAATGAAGAAGAAGATCCCAGTGGTGGTAGA
CAGGACTGGCAACCCAGGACAGAAGGTGTTGAGATCACTGTAACTTTTCCAAGAGATGTCAGTCCTCCCC
AAGAAATGAGCCAAGAAGACTTAAAAGAAAAGAATCTGATAAACTCATCGCTTCAAGAATGGGCACAAGC
ACATGCAGTTTCTCATCCAAATGAAATAGAAACGGTGGAGCTCAGGAAAAAGAAGCTGACCATGCGGCCC
TTAGTTTTGCAAAAAGAGGAAAGTTCCAGGGAGCTCTGCAATGTGAACTTGGGCTTTTTGCTACCAAGAT
CTTGTTTAGAACTGAACATTTCCAAGTCTGTAACCAGAGAAGATGCTCCTCATTTTCTGAAGGAGCAGCA
AAGAAAATCTGAAGAGTTTTCGACCTCTCATATGAAGTACAGTGGCCGAAGCATCAAGAGGCATAGTAGT
GGGCTCAGGATATATGACAGGGAGGAGAAATTTCTCATCTCAAATGAAAAGAAGATATTTTCTGAAAATA
GTTTAAAGTCTGAAGAACCTATCCTATGGACCAAGGGTGAGATTCTTGGAAAGGGAGCCTACGGCACAGT
ATACTGTGGTCTCACTAGTCAAGGACAGCTAATAGCTGTAAAACAGGTGGCTTTGGATACCTCTAATAAA
TTAGCTGCTGAAAAGGAATACCGGAAACTACAGGAAGAAGTAGATTTGCTCAAAGCACTGAAACATGTCA
ACATTGTGGCCTATTTGGGGACATGCTTGCAAGAGAACACTGTGAGCATTTTCATGGAGTTTGTTCCTGG
TGGCTCAATCTCTAGTATTATAAACCGTTTTGGGCCATTGCCTGAGATGGTGTTCTGTAAATATACGAAA
CAAATACTTCAAGGTGTTGCTTATCTCCATGAGAACTGTGTGGTACATCGCGATATCAAAGGAAATAATG
TTATGCTCATGCCAACTGGAATAATAAAGCTGATTGACTTTGGCTGTGCCAGGCGTTTGGCCTGGGCAGG
TTTAAATGGCACCCACAGTGACATGCTTAAGTCCATGCATGGGACTCCATATTGGATGGCCCCAGAAGTC
ATCAATGAGTCTGGCTATGGACGGAAATCAGATATCTGGAGCATTGGTTGTACTGTGTTTGAGATGGCTA
CAGGGAAGCCTCCACTGGCTTCCATGGACAGGATGGCCGCCATGTTTTACATCGGAGCACACCGAGGGCT
GATGCCTCCTTTACCAGACCACTTCTCAGAAAATGCAGCAGACTTTGTGCGCATGTGCCTGACCAGGGAC
CAGCATGAGCGACCTTCTGCTCTCCAGCTCCTGAAGCACTCCTTCTTGGAGAGAAGTCAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC219370 representing NM_001018046
Red=Cloning site Green=Tags(s)

MSSMPKPERHAESLLDICHDTNSSPTDLMTVTKNQNIILQSISRSEEFDQDGDCSHSTLVNEEEDPSGGR
QDWQPRTEGVEITVTFPRDVSPPQEMSQEDLKEKNLINSSLQEWAQAHAVSHPNEIETVELRKKKLTMRP
LVLQKEESSRELCNVNLGFLLPRSCLELNISKSVTREDAPHFLKEQQRKSEEFSTSHMKYSGRSIKRHSS
GLRIYDREEKFLISNEKKIFSENSLKSEEPILWTKGEILGKGAYGTVYCGLTSQGQLIAVKQVALDTSNK
LAAEKEYRKLQEEVDLLKALKHVNIVAYLGTCLQENTVSIFMEFVPGGSISSIINRFGPLPEMVFCKYTK
QILQGVAYLHENCVVHRDIKGNNVMLMPTGIIKLIDFGCARRLAWAGLNGTHSDMLKSMHGTPYWMAPEV
INESGYGRKSDIWSIGCTVFEMATGKPPLASMDRMAAMFYIGAHRGLMPPLPDHFSENAADFVRMCLTRD
QHERPSALQLLKHSFLERSH

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001018046
ORF Size 1530 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001018046.3
RefSeq Size 1711 bp
RefSeq ORF 1533 bp
Locus ID 80122
UniProt ID Q56UN5
Cytogenetics 2q21.3
Protein Families Druggable Genome, Protein Kinase
MW 57.5 kDa

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