EED (NM_003797) Human Tagged ORF Clone

CAT#: RC219261

EED (Myc-DDK-tagged)-Human embryonic ectoderm development (EED), transcript variant 1

Product Images

Circular map for RC219261

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  Western blot validation of overexpression lysate (Cat# LY401248) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC219261 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified EED protein (Cat# TP319261). The protein was produced from HEK293T cells transfected with EED cDNA clone (Cat# RC219261) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: EED
• Synonyms: COGIS; HEED; WAIT1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC219261 representing NM_003797
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCGAGAGGGAAGTGTCGACTGCGCCGGCGGGAACAGACATGCCTGCGGCCAAGAAGCAGAAGCTGA
  GCAGTGACGAGAACAGCAATCCAGACCTCTCTGGAGACGAGAATGATGACGCTGTCAGTATAGAAAGTGG
  TACAAACACTGAACGCCCTGATACACCTACAAACACGCCAAATGCACCTGGAAGGAAAAGTTGGGGAAAG
  GGAAAATGGAAGTCAAAGAAATGCAAATATTCTTTCAAATGTGTAAATAGTCTCAAGGAAGATCATAACC
  AACCATTGTTTGGAGTTCAGTTTAACTGGCACAGTAAAGAAGGAGATCCATTAGTGTTTGCAACTGTAGG
  AAGCAACAGAGTTACCTTGTATGAATGTCATTCACAAGGAGAAATCCGGTTGTTGCAATCTTACGTGGAT
  GCTGATGCTGATGAAAACTTTTACACTTGTGCATGGACCTATGATAGCAATACGAGCCATCCTCTGCTGG
  CTGTAGCTGGATCTAGAGGCATAATTAGGATAATAAATCCTATAACAATGCAGTGTATAAAGCACTATGT
  TGGCCATGGAAATGCTATCAATGAGCTGAAATTCCATCCAAGAGATCCAAATCTTCTCCTGTCAGTAAGT
  AAAGATCATGCTTTACGATTATGGAATATCCAGACGGACACTCTGGTGGCAATATTTGGAGGCGTAGAAG
  GGCACAGAGATGAAGTTCTAAGTGCTGATTATGATCTTTTGGGTGAAAAAATAATGTCCTGTGGTATGGA
  TCATTCTCTTAAACTTTGGAGGATCAATTCAAAGAGAATGATGAATGCAATTAAGGAATCTTATGATTAT
  AATCCAAATAAAACTAACAGGCCATTTATTTCTCAGAAAATCCATTTTCCTGATTTTTCTACCAGAGACA
  TACATAGGAATTATGTTGATTGTGTGCGATGGTTAGGCGATTTGATACTTTCTAAGTCTTGTGAAAATGC
  CATTGTGTGCTGGAAACCTGGCAAGATGGAAGATGATATAGATAAAATTAAACCCAGTGAATCTAATGTG
  ACTATTCTTGGGCGATTTGATTACAGCCAGTGTGACATTTGGTACATGAGGTTTTCTATGGATTTCTGGC
  AAAAGATGCTTGCATTGGGCAATCAAGTTGGCAAACTTTATGTTTGGGATTTAGAAGTAGAAGATCCTCA
  TAAAGCCAAATGTACAACACTGACTCATCATAAATGTGGTGCTGCTATTCGACAAACCAGTTTTAGCAGG
  GATAGCAGCATTCTTATAGCTGTTTGTGATGATGCCAGTATTTGGCGCTGGGATCGACTTCGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC219261 representing NM_003797
  Red=Cloning site Green=Tags(s)
  
  MSEREVSTAPAGTDMPAAKKQKLSSDENSNPDLSGDENDDAVSIESGTNTERPDTPTNTPNAPGRKSWGK
  GKWKSKKCKYSFKCVNSLKEDHNQPLFGVQFNWHSKEGDPLVFATVGSNRVTLYECHSQGEIRLLQSYVD
  ADADENFYTCAWTYDSNTSHPLLAVAGSRGIIRIINPITMQCIKHYVGHGNAINELKFHPRDPNLLLSVS
  KDHALRLWNIQTDTLVAIFGGVEGHRDEVLSADYDLLGEKIMSCGMDHSLKLWRINSKRMMNAIKESYDY
  NPNKTNRPFISQKIHFPDFSTRDIHRNYVDCVRWLGDLILSKSCENAIVCWKPGKMEDDIDKIKPSESNV
  TILGRFDYSQCDIWYMRFSMDFWQKMLALGNQVGKLYVWDLEVEDPHKAKCTTLTHHKCGAAIRQTSFSR
  DSSILIAVCDDASIWRWDRLR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003797
• ORF Size: 1323 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003797.5
• RefSeq Size: 2006 bp
• RefSeq ORF: 1326 bp
• Locus ID: 8726
• UniProt ID: O75530
• Cytogenetics: 11q14.2
• Domains: WD40
• Protein Families: Druggable Genome, Transcription Factors
• MW: 50 kDa
• Gene Summary: This gene encodes a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein interacts with enhancer of zeste 2, the cytoplasmic tail of integrin beta7, immunodeficiency virus type 1 (HIV-1) MA protein, and histone deacetylase proteins. This protein mediates repression of gene activity through histone deacetylation, and may act as a specific regulator of integrin function. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]