Aldehyde Oxidase (AOX1) (NM_001159) Human Tagged ORF Clone

CAT#: RC219221

AOX1 (Myc-DDK-tagged)-Human aldehyde oxidase 1 (AOX1)

Product Images

Circular map for RC219221

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY AOX1 (Cat# RC219221, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AOX1 antibody (Cat# TA890040). Positive lysates LY400466 (100ug) and LC400466 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY400466) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC219221 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified AOX1 protein (Cat# TP319221). The protein was produced from HEK293T cells transfected with AOX1 cDNA clone (Cat# RC219221) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Aldehyde Oxidase
• Synonyms: AO; AOH1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC219221 representing NM_001159
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACCGGGCGTCCGAGCTGCTCTTCTACGTGAACGGCCGCAAGGTGATAGAAAAAAATGTCGATCCTG
  AAACAATGCTGTTGCCTTATTTGAGGAAGAAGCTTCGACTCACAGGAACTAAGTATGGCTGTGGAGGAGG
  AGGCTGTGGTGCTTGTACAGTGATGATATCACGATACAACCCCATCACCAAGAGGATAAGGCATCACCCA
  GCCAATGCCTGTCTGATTCCCATCTGTTCTCTGTATGGTGCTGCCGTCACCACAGTAGAAGGCATAGGAA
  GCACCCACACCAGAATTCATCCTGTTCAGGAGAGGATTGCCAAGTGTCATGGCACCCAGTGTGGCTTCTG
  CACACCTGGGATGGTGATGTCCATCTACACGCTGCTCAGGAACCACCCAGAGCCCACTCTGGATCAGTTA
  ACTGATGCCCTTGGTGGTAACCTGTGCCGTTGCACTGGATACAGGCCCATAATTGATGCATGCAAGACTT
  TCTGTAAAACTTCGGGCTGCTGTCAAAGTAAAGAAAATGGGGTTTGCTGTTTGGATCAAGGAATCAATGG
  ATTGCCAGAATTTGAGGAAGGAAGTAAGACAAGTCCAAAACTCTTCGCAGAAGAGGAGTTTCTGCCATTG
  GATCCAACCCAGGAACTGATATTTCCTCCTGAGCTAATGATAATGGCTGAGAAACAGTCGCAAAGGACCA
  GGGTGTTTGGCAGTGAGAGAATGATGTGGTTTTCCCCCGTGACCCTGAAGGAACTGCTGGAATTTAAATT
  CAAGTATCCCCAGGCTCCTGTTATCATGGGAAACACCTCTGTGGGGCCTGAAGTGAAATTTAAAGGCGTC
  TTTCACCCAGTTATAATTTCTCCTGATAGAATTGAAGAACTGAGTGTTGTAAACCATGCATATAATGGAC
  TCACCCTTGGTGCTGGTCTCAGCCTAGCCCAGGTGAAGGACATTTTGGCTGATGTAGTCCAGAAGCTTCC
  AGAGGAGAAGACACAGATGTACCATGCTCTCCTGAAGCATTTGGGAACTCTGGCTGGGTCCCAGATCAGG
  AACATGGCTTCTTTAGGGGGACACATCATTAGCAGGCATCCAGATTCAGATCTGAATCCCATCCTGGCTG
  TGGGTAACTGTACCCTCAACTTGCTATCAAAAGAAGGAAAACGACAGATTCCTTTAAATGAGCAATTCCT
  CAGCAAGTGCCCTAATGCAGATCTTAAGCCTCAAGAAATCTTGGTCTCAGTGAACATCCCCTACTCAAGG
  AAGTGGGAATTTGTGTCAGCCTTCCGACAAGCCCAGCGACAGGAGAATGCGCTAGCGATAGTCAATTCAG
  GAATGAGAGTCTTTTTTGGAGAAGGGGATGGCATTATTAGAGAGTTATGCATCTCATATGGAGGCGTTGG
  TCCAGCCACCATCTGTGCCAAGAATTCCTGCCAGAAACTCATTGGAAGGCACTGGAACGAACAGATGCTG
  GATATAGCCTGCAGGCTTATTCTGAATGAAGTCTCCCTTTTGGGCTCGGCGCCAGGTGGGAAAGTGGAGT
  TCAAGAGGACTCTCATCATCAGCTTCCTCTTCAAGTTCTACCTGGAAGTGTCACAGATTTTGAAAAAGAT
  GGATCCAGTTCACTATCCTAGCCTTGCAGACAAGTATGAAAGTGCTTTAGAAGATCTTCATTCCAAACAT
  CACTGCAGTACATTAAAGTACCAGAATATAGGCCCAAAGCAGCATCCTGAAGACCCAATTGGCCACCCCA
  TCATGCATCTGTCTGGTGTGAAGCATGCCACGGGGGAGGCCATCTACTGTGATGACATGCCTCTGGTGGA
  CCAGGAACTTTTCTTGACTTTTGTGACTAGTTCAAGAGCTCATGCTAAGATTGTGTCTATTGATCTGTCA
  GAAGCTCTCAGCATGCCCGGTGTGGTGGACATCATGACAGCAGAACATCTTAGTGACGTCAACTCCTTCT
  GCTTTTTTACTGAAGCTGAGAAATTTCTGGCGACAGATAAGGTGTTCTGTGTGGGTCAGCTTGTCTGTGC
  TGTGCTTGCCGATTCTGAGGTTCAGGCAAAGCGAGCTGCTAAGCGAGTGAAGATTGTCTATCAAGACTTG
  GAGCCGCTGATACTAACAATTGAGGAAAGTATACAACACAACTCCTCCTTCAAGCCAGAAAGGAAACTGG
  AATATGGAAATGTTGACGAAGCATTTAAAGTGGTTGATCAAATTCTTGAAGGTGAAATACATATGGGAGG
  TCAAGAACATTTTTATATGGAAACCCAAAGCATGCTTGTCGTTCCCAAGGGAGAGGATCAAGAAATGGAT
  GTCTACGTGTCCACACAGTTTCCCAAATATATACAGGACATTGTTGCCTCAACCTTGAAGCTCCCAGCTA
  ACAAGGTCATGTGCCATGTAAGGCGTGTTGGTGGAGCGTTTGGAGGGAAGGTGTTAAAAACCGGAATCAT
  TGCAGCCGTCACTGCATTTGCCGCAAACAAACATGGCCGTGCAGTTCGCTGTGTTCTGGAACGAGGAGAA
  GACATGTTAATAACTGGAGGCCGCCATCCTTACCTTGGAAAGTACAAAGCTGGATTCATGAACGATGGCA
  GAATCTTGGCCCTGGACATGGAGCATTACAGCAATGCAGGCGCCTCCTTGGATGAATCATTATTCGTGAT
  AGAAATGGGACTTCTGAAAATGGACAATGCTTACAAGTTTCCCAATCTCCGCTGCCGGGGTTGGGCATGC
  AGAACCAACCTTCCATCCAACACAGCTTTTCGTGGGTTTGGCTTTCCTCAGGCAGCGCTGATCACCGAAT
  CTTGTATCACGGAAGTTGCAGCCAAATGTGGACTATCCCCTGAGAAGGTGCGAATCATAAACATGTACAA
  GGAAATTGATCAAACACCCTACAAACAAGAGATCAATGCCAAGAACCTAATCCAGTGTTGGAGAGAATGT
  ATGGCCATGTCTTCCTACTCCTTGAGGAAAGTTGCTGTGGAAAAGTTCAATGCAGAGAATTATTGGAAGA
  AGAAAGGACTGGCCATGGTCCCCCTGAAGTTTCCTGTTGGCCTTGGCTCACGTGCTGCTGGTCAGGCTGC
  TGCCTTGGTTCACATTTATCTTGATGGCTCTGTGCTGGTCACTCACGGTGGAATTGAAATGGGGCAGGGG
  GTCCACACTAAAATGATTCAGGTGGTCAGCCGTGAATTAAGAATGCCAATGTCGAATGTCCACCTGCGTG
  GAACAAGCACAGAAACTGTCCCTAATGCAAATATCTCTGGAGGTTCTGTGGTGGCAGATCTCAACGGTTT
  GGCAGTAAAGGATGCCTGTCAAACTCTTCTAAAACGCCTCGAACCCATCATCAGCAAGAATCCTAAAGGA
  ACTTGGAAAGACTGGGCACAGACTGCTTTTGATGAAAGCATTAACCTTTCAGCTGTTGGATACTTCAGAG
  GTTATGAGTCAGACATGAACTGGGAGAAAGGCGAAGGCCAGCCCTTCGAATACTTTGTTTATGGAGCTGC
  CTGTTCCGAGGTTGAAATAGACTGCCTGACGGGGGATCATAAGAACATCAGAACAGACATTGTCATGGAT
  GTTGGCTGCAGTATAAATCCAGCCATTGACATAGGCCAGATTGAAGGTGCATTTATTCAAGGCATGGGAC
  TTTATACAATAGAGGAACTGAATTATTCTCCCCAGGGCATTCTGCACACTCGTGGTCCAGACCAATATAA
  AATCCCTGCCATCTGTGACATGCCCACGGAGTTGCACATTGCTTTGTTGCCTCCTTCTCAAAACTCAAAT
  ACTCTTTATTCATCTAAGGGTCTGGGAGAGTCGGGGGTGTTCCTGGGGTGTTCCGTGTTTTTCGCTATCC
  ATGACGCAGTGAGTGCAGCACGACAGGAGAGAGGCCTGCATGGACCCTTGACCCTTAATAGTCCACTGAC
  CCCGGAGAAGATTAGGATGGCCTGTGAAGACAAGTTCACAAAAATGATTCCGAGAGATGAACCTGGATCC
  TACGTTCCTTGGAATGTACCCATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC219221 representing NM_001159
  Red=Cloning site Green=Tags(s)
  
  MDRASELLFYVNGRKVIEKNVDPETMLLPYLRKKLRLTGTKYGCGGGGCGACTVMISRYNPITKRIRHHP
  ANACLIPICSLYGAAVTTVEGIGSTHTRIHPVQERIAKCHGTQCGFCTPGMVMSIYTLLRNHPEPTLDQL
  TDALGGNLCRCTGYRPIIDACKTFCKTSGCCQSKENGVCCLDQGINGLPEFEEGSKTSPKLFAEEEFLPL
  DPTQELIFPPELMIMAEKQSQRTRVFGSERMMWFSPVTLKELLEFKFKYPQAPVIMGNTSVGPEVKFKGV
  FHPVIISPDRIEELSVVNHAYNGLTLGAGLSLAQVKDILADVVQKLPEEKTQMYHALLKHLGTLAGSQIR
  NMASLGGHIISRHPDSDLNPILAVGNCTLNLLSKEGKRQIPLNEQFLSKCPNADLKPQEILVSVNIPYSR
  KWEFVSAFRQAQRQENALAIVNSGMRVFFGEGDGIIRELCISYGGVGPATICAKNSCQKLIGRHWNEQML
  DIACRLILNEVSLLGSAPGGKVEFKRTLIISFLFKFYLEVSQILKKMDPVHYPSLADKYESALEDLHSKH
  HCSTLKYQNIGPKQHPEDPIGHPIMHLSGVKHATGEAIYCDDMPLVDQELFLTFVTSSRAHAKIVSIDLS
  EALSMPGVVDIMTAEHLSDVNSFCFFTEAEKFLATDKVFCVGQLVCAVLADSEVQAKRAAKRVKIVYQDL
  EPLILTIEESIQHNSSFKPERKLEYGNVDEAFKVVDQILEGEIHMGGQEHFYMETQSMLVVPKGEDQEMD
  VYVSTQFPKYIQDIVASTLKLPANKVMCHVRRVGGAFGGKVLKTGIIAAVTAFAANKHGRAVRCVLERGE
  DMLITGGRHPYLGKYKAGFMNDGRILALDMEHYSNAGASLDESLFVIEMGLLKMDNAYKFPNLRCRGWAC
  RTNLPSNTAFRGFGFPQAALITESCITEVAAKCGLSPEKVRIINMYKEIDQTPYKQEINAKNLIQCWREC
  MAMSSYSLRKVAVEKFNAENYWKKKGLAMVPLKFPVGLGSRAAGQAAALVHIYLDGSVLVTHGGIEMGQG
  VHTKMIQVVSRELRMPMSNVHLRGTSTETVPNANISGGSVVADLNGLAVKDACQTLLKRLEPIISKNPKG
  TWKDWAQTAFDESINLSAVGYFRGYESDMNWEKGEGQPFEYFVYGAACSEVEIDCLTGDHKNIRTDIVMD
  VGCSINPAIDIGQIEGAFIQGMGLYTIEELNYSPQGILHTRGPDQYKIPAICDMPTELHIALLPPSQNSN
  TLYSSKGLGESGVFLGCSVFFAIHDAVSAARQERGLHGPLTLNSPLTPEKIRMACEDKFTKMIPRDEPGS
  YVPWNVPI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001159
• ORF Size: 4014 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001159.4
• RefSeq Size: 4949 bp
• RefSeq ORF: 4017 bp
• Locus ID: 316
• UniProt ID: Q06278
• Cytogenetics: 2q33.1
• Domains: Ald_Xan_dh_C, fer2, FAD_binding_5, fer2_2, CO_deh_flav_C
• Protein Families: Druggable Genome
• Protein Pathways: Drug metabolism - cytochrome P450, Metabolic pathways, Nicotinate and nicotinamide metabolism, Tryptophan metabolism, Tyrosine metabolism, Valine, leucine and isoleucine degradation, Vitamin B6 metabolism
• MW: 147.7 kDa
• Gene Summary: Aldehyde oxidase produces hydrogen peroxide and, under certain conditions, can catalyze the formation of superoxide. Aldehyde oxidase is a candidate gene for amyotrophic lateral sclerosis. [provided by RefSeq, Jul 2008]