NANOS1 (NM_199461) Human Tagged ORF Clone

CAT#: RC219123

NANOS1 (Myc-DDK-tagged)-Human nanos homolog 1 (Drosophila) (NANOS1)

Product Images

Circular map for RC219123

• 

  Western blot validation of overexpression lysate (Cat# LY404576) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC219123 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NANOS1
• Synonyms: EC_Rep1a; NOS-1; NOS1; SPGF12; ZC2HC12A
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC219123 representing NM_199461
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGCTTTCCCCTGGGCGCCCCGCTCGCCCCGCCGCGGCCGCGCCCCCCCGCCCATGGCGCTCGTGC
  CCAGCGCCCGCTACGTGAGCGCCCCGGGCCCGGCGCACCCGCAGCCCTTCAGCTCCTGGAACGACTACCT
  GGGGCTCGCCACGCTCATCACCAAAGCGGTGGACGGCGAGCCGCGCTTCGGCTGCGCCCGCGGTGGGAAC
  GGCGGCGGCGGCTCCCCGCCCTCCTCCTCCTCGTCGTCCTGCTGCTCCCCCCACACGGGGGCCGGGCCTG
  GGGCGCTGGGGCCGGCGCTGGGGCCGCCCGACTACGACGAGGACGACGACGACGACAGCGACGAGCCGGG
  GTCCCGGGGCCGCTACCTGGGGAGCGCGCTGGAATTGCGCGCGCTGGAGCTGTGCGCGGGCCCCGCCGAG
  GCCGGGCTGCTGGAGGAGCGCTTCGCCGAGCTGAGCCCGTTCGCGGGTCGTGCCGCCGCCGTGCTGCTGG
  GCTGCGCGCCCGCCGCCGCCGCCGCCGCCACCACCACCAGCGAGGCGACGCCGCGCGAGGAGCGGGCCCC
  GGCGTGGGCGGCCGAGCCCCGGCTGCACGCGGCCTCCGGGGCGGCGGCCGCCCGGCTGCTGAAGCCCGAG
  CTGCAGGTGTGCGTGTTCTGCCGGAACAACAAGGAGGCGATGGCGCTCTACACCACCCATATCCTCAAGG
  GCCCCGACGGGCGAGTGCTGTGTCCCGTGCTGCGCCGCTACACGTGTCCCCTGTGCGGCGCCAGCGGCGA
  CAACGCGCACACCATCAAGTACTGCCCGCTCTCCAAAGTGCCGCCGCCGCCCGCCCGCCCGCCGCCCCGC
  AGCGCCAGGGACGGCCCGCCTGGCAAGAAGCTGCGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC219123 representing NM_199461
  Red=Cloning site Green=Tags(s)
  
  MEAFPWAPRSPRRGRAPPPMALVPSARYVSAPGPAHPQPFSSWNDYLGLATLITKAVDGEPRFGCARGGN
  GGGGSPPSSSSSSCCSPHTGAGPGALGPALGPPDYDEDDDDDSDEPGSRGRYLGSALELRALELCAGPAE
  AGLLEERFAELSPFAGRAAAVLLGCAPAAAAAATTTSEATPREERAPAWAAEPRLHAASGAAAARLLKPE
  LQVCVFCRNNKEAMALYTTHILKGPDGRVLCPVLRRYTCPLCGASGDNAHTIKYCPLSKVPPPPARPPPR
  SARDGPPGKKLR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_199461
• ORF Size: 876 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.

Reference Data

• RefSeq: NM_199461.4
• RefSeq Size: 4627 bp
• RefSeq ORF: 879 bp
• Locus ID: 340719
• UniProt ID: Q8WY41
• Cytogenetics: 10q26.11
• MW: 30 kDa
• Gene Summary: This gene encodes a CCHC-type zinc finger protein that is a member of the nanos family. This protein co-localizes with the RNA-binding protein pumilio RNA-binding family member 2 and may be involved in regulating translation as a post-transcriptional repressor. Mutations in this gene are associated with spermatogenic impairment. [provided by RefSeq, Sep 2015]