CACNA2D1 (NM_000722) Human Tagged ORF Clone

CAT#: RC219081

CACNA2D1 (Myc-DDK-tagged)-Human calcium channel, voltage-dependent, alpha 2/delta subunit 1 (CACNA2D1)

Product Images

Circular map for RC219081

• 

  Western blot validation of overexpression lysate (Cat# LY424551) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC219081 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CACNA2D1
• Synonyms: CACNA2; CACNL2A; CCHL2A; LINC01112; lncRNA-N3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC219081 representing NM_000722
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGCTGGCTGCCTGCTGGCCTTGACTCTGACACTTTTCCAATCTTTGCTCATCGGCCCCTCGTCGG
  AGGAGCCGTTCCCTTCGGCCGTCACTATCAAATCATGGGTGGATAAGATGCAAGAAGACCTTGTCACACT
  GGCAAAAACAGCAAGTGGAGTCAATCAGCTTGTTGATATTTATGAGAAATATCAAGATTTGTATACTGTG
  GAACCAAATAATGCACGCCAGCTGGTAGAAATTGCAGCCAGGGATATTGAGAAACTTCTGAGCAACAGAT
  CTAAAGCCCTGGTGCGCCTGGCATTGGAAGCGGAGAAAGTTCAAGCAGCTCACCAGTGGAGAGAAGATTT
  TGCAAGCAATGAAGTTGTCTACTACAATGCAAAGGATGATCTCGATCCTGAGAAAAATGACAGTGAGCCA
  GGCAGCCAGAGGATAAAACCTGTTTTCATTGAAGATGCTAATTTTGGACGACAAATATCTTATCAGCACG
  CAGCAGTCCATATTCCTACTGACATCTATGAGGGCTCAACAATTGTGTTAAATGAACTCAACTGGACAAG
  TGCCTTAGATGAAGTTTTCAAAAAGAATCGCGAGGAAGACCCTTCATTATTGTGGCAGGTTTTTGGCAGT
  GCCACTGGCCTAGCTCGATATTATCCAGCTTCACCATGGGTTGATAATAGTAGAACTCCAAATAAGATTG
  ACCTTTATGATGTACGCAGAAGACCATGGTACATCCAAGGAGCTGCATCTCCTAAAGACATGCTTATTCT
  GGTGGATGTGAGTGGAAGTGTTAGTGGATTGACACTTAAACTGATCCGAACATCTGTCTCCGAAATGTTA
  GAAACCCTCTCAGATGATGATTTCGTGAATGTAGCTTCATTTAACAGCAATGCTCAGGATGTAAGCTGTT
  TTCAGCACCTTGTCCAAGCAAATGTAAGAAATAAAAAAGTGTTGAAAGACGCGGTGAATAATATCACAGC
  CAAAGGAATTACAGATTATAAGAAGGGCTTTAGTTTTGCTTTTGAACAGCTGCTTAATTATAATGTTTCC
  AGAGCAAACTGCAATAAGATTATTATGCTATTCACGGATGGAGGAGAAGAGAGAGCCCAGGAGATATTTA
  ACAAATACAATAAAGATAAAAAAGTACGTGTATTCACGTTTTCAGTTGGTCAACACAATTATGACAGAGG
  ACCTATTCAGTGGATGGCCTGTGAAAACAAAGGTTATTATTATGAAATTCCTTCCATTGGTGCAATAAGA
  ATCAATACTCAGGAATATTTGGATGTTTTGGGAAGACCAATGGTTTTAGCAGGAGACAAAGCTAAGCAAG
  TCCAATGGACAAATGTGTACCTGGATGCATTGGAACTGGGACTTGTCATTACTGGAACTCTTCCGGTCTT
  CAACATAACCGGCCAATTTGAAAATAAGACAAACTTAAAGAACCAGCTGATTCTTGGTGTGATGGGAGTA
  GATGTGTCTTTGGAAGATATTAAAAGACTGACACCACGTTTTACACTGTGCCCCAATGGGTATTACTTTG
  CAATCGATCCTAATGGTTATGTTTTATTACATCCAAATCTTCAGCCAAAGAACCCCAAATCTCAGGAGCC
  AGTAACATTGGATTTCCTTGATGCAGAGTTAGAGAATGATATTAAAGTGGAGATTCGAAATAAGATGATT
  GATGGGGAAAGTGGAGAAAAAACATTCAGAACTCTGGTTAAATCTCAAGATGAGAGATATATTGACAAAG
  GAAACAGGACATACACATGGACACCTGTCAATGGCACAGATTACAGTTTGGCCTTGGTATTACCAACCTA
  CAGTTTTTACTATATAAAAGCCAAACTAGAAGAGACAATAACTCAGGCCAGATCAAAAAAGGGCAAAATG
  AAGGATTCGGAAACCCTGAAGCCAGATAATTTTGAAGAATCTGGCTATACATTCATAGCACCAAGAGATT
  ACTGCAATGACCTGAAAATATCGGATAATAACACTGAATTTCTTTTAAATTTCAACGAGTTTATTGATAG
  AAAAACTCCAAACAACCCATCATGTAACGCGGATTTGATTAATAGAGTCTTGCTTGATGCAGGCTTTACA
  AATGAACTTGTCCAAAATTACTGGAGTAAGCAGAAAAATATCAAGGGAGTGAAAGCACGATTTGTTGTGA
  CTGATGGTGGGATTACCAGAGTTTATCCCAAAGAGGCTGGAGAAAATTGGCAAGAAAACCCAGAGACATA
  TGAGGACAGCTTCTATAAAAGGAGCCTAGATAATGATAACTATGTTTTCACTGCTCCCTACTTTAACAAA
  AGTGGACCTGGTGCCTATGAATCGGGCATTATGGTAAGCAAAGCTGTAGAAATATATATTCAAGGGAAAC
  TTCTTAAACCTGCAGTTGTTGGAATTAAAATTGATGTAAATTCCTGGATAGAGAATTTCACCAAAACCTC
  AATCAGAGATCCGTGTGCTGGTCCAGTTTGTGACTGCAAAAGAAACAGTGACGTAATGGATTGTGTGATT
  CTGGATGATGGTGGGTTTCTTCTGATGGCAAATCATGATGATTATACTAATCAGATTGGAAGATTTTTTG
  GAGAGATTGATCCCAGCTTGATGAGACACCTGGTTAATATATCAGTTTATGCTTTTAACAAATCTTATGA
  TTATCAGTCAGTATGTGAGCCCGGTGCTGCACCAAAACAAGGAGCAGGACATCGCTCAGCATATGTGCCA
  TCAGTAGCAGACATATTACAAATTGGCTGGTGGGCCACTGCTGCTGCCTGGTCTATTCTACAGCAGTTTC
  TCTTGAGTTTGACCTTTCCACGACTCCTTGAGGCAGTTGAGATGGAGGATGATGACTTCACGGCCTCCCT
  GTCCAAGCAGAGCTGCATTACTGAACAAACCCAGTATTTCTTCGATAACGACAGTAAATCATTCAGTGGT
  GTATTAGACTGTGGAAACTGTTCCAGAATCTTTCATGGAGAAAAGCTTATGAACACCAACTTAATATTCA
  TAATGGTTGAGAGCAAAGGGACATGTCCATGTGACACACGACTGCTCATACAAGCGGAGCAGACTTCTGA
  CGGTCCAAATCCTTGTGACATGGTTAAGCAACCTAGATACCGAAAAGGGCCTGATGTCTGCTTTGATAAC
  AATGTCTTGGAGGATTATACTGACTGTGGTGGTGTTTCTGGATTAAATCCCTCCCTGTGGTATATCATTG
  GAATCCAGTTTCTACTACTTTGGCTGGTATCTGGCAGCACACACCGCCTGTTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC219081 representing NM_000722
  Red=Cloning site Green=Tags(s)
  
  MAAGCLLALTLTLFQSLLIGPSSEEPFPSAVTIKSWVDKMQEDLVTLAKTASGVNQLVDIYEKYQDLYTV
  EPNNARQLVEIAARDIEKLLSNRSKALVRLALEAEKVQAAHQWREDFASNEVVYYNAKDDLDPEKNDSEP
  GSQRIKPVFIEDANFGRQISYQHAAVHIPTDIYEGSTIVLNELNWTSALDEVFKKNREEDPSLLWQVFGS
  ATGLARYYPASPWVDNSRTPNKIDLYDVRRRPWYIQGAASPKDMLILVDVSGSVSGLTLKLIRTSVSEML
  ETLSDDDFVNVASFNSNAQDVSCFQHLVQANVRNKKVLKDAVNNITAKGITDYKKGFSFAFEQLLNYNVS
  RANCNKIIMLFTDGGEERAQEIFNKYNKDKKVRVFTFSVGQHNYDRGPIQWMACENKGYYYEIPSIGAIR
  INTQEYLDVLGRPMVLAGDKAKQVQWTNVYLDALELGLVITGTLPVFNITGQFENKTNLKNQLILGVMGV
  DVSLEDIKRLTPRFTLCPNGYYFAIDPNGYVLLHPNLQPKNPKSQEPVTLDFLDAELENDIKVEIRNKMI
  DGESGEKTFRTLVKSQDERYIDKGNRTYTWTPVNGTDYSLALVLPTYSFYYIKAKLEETITQARSKKGKM
  KDSETLKPDNFEESGYTFIAPRDYCNDLKISDNNTEFLLNFNEFIDRKTPNNPSCNADLINRVLLDAGFT
  NELVQNYWSKQKNIKGVKARFVVTDGGITRVYPKEAGENWQENPETYEDSFYKRSLDNDNYVFTAPYFNK
  SGPGAYESGIMVSKAVEIYIQGKLLKPAVVGIKIDVNSWIENFTKTSIRDPCAGPVCDCKRNSDVMDCVI
  LDDGGFLLMANHDDYTNQIGRFFGEIDPSLMRHLVNISVYAFNKSYDYQSVCEPGAAPKQGAGHRSAYVP
  SVADILQIGWWATAAAWSILQQFLLSLTFPRLLEAVEMEDDDFTASLSKQSCITEQTQYFFDNDSKSFSG
  VLDCGNCSRIFHGEKLMNTNLIFIMVESKGTCPCDTRLLIQAEQTSDGPNPCDMVKQPRYRKGPDVCFDN
  NVLEDYTDCGGVSGLNPSLWYIIGIQFLLLWLVSGSTHRLL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_000722
• ORF Size: 3273 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000722.4
• RefSeq Size: 3822 bp
• RefSeq ORF: 3276 bp
• Locus ID: 781
• UniProt ID: P54289
• Cytogenetics: 7q21.11
• Domains: VWA, Cache
• Protein Families: Druggable Genome, Ion Channels: Other
• Protein Pathways: Arrhythmogenic right ventricular cardiomyopathy (ARVC), Cardiac muscle contraction, Dilated cardiomyopathy, Hypertrophic cardiomyopathy (HCM), MAPK signaling pathway
• MW: 123 kDa
• Gene Summary: The preproprotein encoded by this gene is cleaved into multiple chains that comprise the alpha-2 and delta subunits of the voltage-dependent calcium channel complex. Calcium channels mediate the influx of calcium ions into the cell upon membrane polarization. Mutations in this gene can cause cardiac deficiencies, including Brugada syndrome and short QT syndrome. Alternate splicing results in multiple transcript variants, some of which may lack the delta subunit portion. [provided by RefSeq, Nov 2014]