CACNA2D1 (NM_000722) Human Tagged ORF Clone

CAT#: RC219081

Reviews ()
Write a review

CACNA2D1 (Myc-DDK-tagged)-Human calcium channel, voltage-dependent, alpha 2/delta subunit 1 (CACNA2D1)



  "NM_000722" in other vectors (6)

Reconstitution Protocol

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 680.00


Availability*
In Stock

Size
    • 10 ug


Product images

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol CACNA2D1
Synonyms CACNA2; CACNL2A; CCHL2A; LINC01112; lncRNA-N3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC219081 representing NM_000722
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTGCTGGCTGCCTGCTGGCCTTGACTCTGACACTTTTCCAATCTTTGCTCATCGGCCCCTCGTCGG
AGGAGCCGTTCCCTTCGGCCGTCACTATCAAATCATGGGTGGATAAGATGCAAGAAGACCTTGTCACACT
GGCAAAAACAGCAAGTGGAGTCAATCAGCTTGTTGATATTTATGAGAAATATCAAGATTTGTATACTGTG
GAACCAAATAATGCACGCCAGCTGGTAGAAATTGCAGCCAGGGATATTGAGAAACTTCTGAGCAACAGAT
CTAAAGCCCTGGTGCGCCTGGCATTGGAAGCGGAGAAAGTTCAAGCAGCTCACCAGTGGAGAGAAGATTT
TGCAAGCAATGAAGTTGTCTACTACAATGCAAAGGATGATCTCGATCCTGAGAAAAATGACAGTGAGCCA
GGCAGCCAGAGGATAAAACCTGTTTTCATTGAAGATGCTAATTTTGGACGACAAATATCTTATCAGCACG
CAGCAGTCCATATTCCTACTGACATCTATGAGGGCTCAACAATTGTGTTAAATGAACTCAACTGGACAAG
TGCCTTAGATGAAGTTTTCAAAAAGAATCGCGAGGAAGACCCTTCATTATTGTGGCAGGTTTTTGGCAGT
GCCACTGGCCTAGCTCGATATTATCCAGCTTCACCATGGGTTGATAATAGTAGAACTCCAAATAAGATTG
ACCTTTATGATGTACGCAGAAGACCATGGTACATCCAAGGAGCTGCATCTCCTAAAGACATGCTTATTCT
GGTGGATGTGAGTGGAAGTGTTAGTGGATTGACACTTAAACTGATCCGAACATCTGTCTCCGAAATGTTA
GAAACCCTCTCAGATGATGATTTCGTGAATGTAGCTTCATTTAACAGCAATGCTCAGGATGTAAGCTGTT
TTCAGCACCTTGTCCAAGCAAATGTAAGAAATAAAAAAGTGTTGAAAGACGCGGTGAATAATATCACAGC
CAAAGGAATTACAGATTATAAGAAGGGCTTTAGTTTTGCTTTTGAACAGCTGCTTAATTATAATGTTTCC
AGAGCAAACTGCAATAAGATTATTATGCTATTCACGGATGGAGGAGAAGAGAGAGCCCAGGAGATATTTA
ACAAATACAATAAAGATAAAAAAGTACGTGTATTCACGTTTTCAGTTGGTCAACACAATTATGACAGAGG
ACCTATTCAGTGGATGGCCTGTGAAAACAAAGGTTATTATTATGAAATTCCTTCCATTGGTGCAATAAGA
ATCAATACTCAGGAATATTTGGATGTTTTGGGAAGACCAATGGTTTTAGCAGGAGACAAAGCTAAGCAAG
TCCAATGGACAAATGTGTACCTGGATGCATTGGAACTGGGACTTGTCATTACTGGAACTCTTCCGGTCTT
CAACATAACCGGCCAATTTGAAAATAAGACAAACTTAAAGAACCAGCTGATTCTTGGTGTGATGGGAGTA
GATGTGTCTTTGGAAGATATTAAAAGACTGACACCACGTTTTACACTGTGCCCCAATGGGTATTACTTTG
CAATCGATCCTAATGGTTATGTTTTATTACATCCAAATCTTCAGCCAAAGAACCCCAAATCTCAGGAGCC
AGTAACATTGGATTTCCTTGATGCAGAGTTAGAGAATGATATTAAAGTGGAGATTCGAAATAAGATGATT
GATGGGGAAAGTGGAGAAAAAACATTCAGAACTCTGGTTAAATCTCAAGATGAGAGATATATTGACAAAG
GAAACAGGACATACACATGGACACCTGTCAATGGCACAGATTACAGTTTGGCCTTGGTATTACCAACCTA
CAGTTTTTACTATATAAAAGCCAAACTAGAAGAGACAATAACTCAGGCCAGATCAAAAAAGGGCAAAATG
AAGGATTCGGAAACCCTGAAGCCAGATAATTTTGAAGAATCTGGCTATACATTCATAGCACCAAGAGATT
ACTGCAATGACCTGAAAATATCGGATAATAACACTGAATTTCTTTTAAATTTCAACGAGTTTATTGATAG
AAAAACTCCAAACAACCCATCATGTAACGCGGATTTGATTAATAGAGTCTTGCTTGATGCAGGCTTTACA
AATGAACTTGTCCAAAATTACTGGAGTAAGCAGAAAAATATCAAGGGAGTGAAAGCACGATTTGTTGTGA
CTGATGGTGGGATTACCAGAGTTTATCCCAAAGAGGCTGGAGAAAATTGGCAAGAAAACCCAGAGACATA
TGAGGACAGCTTCTATAAAAGGAGCCTAGATAATGATAACTATGTTTTCACTGCTCCCTACTTTAACAAA
AGTGGACCTGGTGCCTATGAATCGGGCATTATGGTAAGCAAAGCTGTAGAAATATATATTCAAGGGAAAC
TTCTTAAACCTGCAGTTGTTGGAATTAAAATTGATGTAAATTCCTGGATAGAGAATTTCACCAAAACCTC
AATCAGAGATCCGTGTGCTGGTCCAGTTTGTGACTGCAAAAGAAACAGTGACGTAATGGATTGTGTGATT
CTGGATGATGGTGGGTTTCTTCTGATGGCAAATCATGATGATTATACTAATCAGATTGGAAGATTTTTTG
GAGAGATTGATCCCAGCTTGATGAGACACCTGGTTAATATATCAGTTTATGCTTTTAACAAATCTTATGA
TTATCAGTCAGTATGTGAGCCCGGTGCTGCACCAAAACAAGGAGCAGGACATCGCTCAGCATATGTGCCA
TCAGTAGCAGACATATTACAAATTGGCTGGTGGGCCACTGCTGCTGCCTGGTCTATTCTACAGCAGTTTC
TCTTGAGTTTGACCTTTCCACGACTCCTTGAGGCAGTTGAGATGGAGGATGATGACTTCACGGCCTCCCT
GTCCAAGCAGAGCTGCATTACTGAACAAACCCAGTATTTCTTCGATAACGACAGTAAATCATTCAGTGGT
GTATTAGACTGTGGAAACTGTTCCAGAATCTTTCATGGAGAAAAGCTTATGAACACCAACTTAATATTCA
TAATGGTTGAGAGCAAAGGGACATGTCCATGTGACACACGACTGCTCATACAAGCGGAGCAGACTTCTGA
CGGTCCAAATCCTTGTGACATGGTTAAGCAACCTAGATACCGAAAAGGGCCTGATGTCTGCTTTGATAAC
AATGTCTTGGAGGATTATACTGACTGTGGTGGTGTTTCTGGATTAAATCCCTCCCTGTGGTATATCATTG
GAATCCAGTTTCTACTACTTTGGCTGGTATCTGGCAGCACACACCGCCTGTTA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC219081 representing NM_000722
Red=Cloning site Green=Tags(s)

MAAGCLLALTLTLFQSLLIGPSSEEPFPSAVTIKSWVDKMQEDLVTLAKTASGVNQLVDIYEKYQDLYTV
EPNNARQLVEIAARDIEKLLSNRSKALVRLALEAEKVQAAHQWREDFASNEVVYYNAKDDLDPEKNDSEP
GSQRIKPVFIEDANFGRQISYQHAAVHIPTDIYEGSTIVLNELNWTSALDEVFKKNREEDPSLLWQVFGS
ATGLARYYPASPWVDNSRTPNKIDLYDVRRRPWYIQGAASPKDMLILVDVSGSVSGLTLKLIRTSVSEML
ETLSDDDFVNVASFNSNAQDVSCFQHLVQANVRNKKVLKDAVNNITAKGITDYKKGFSFAFEQLLNYNVS
RANCNKIIMLFTDGGEERAQEIFNKYNKDKKVRVFTFSVGQHNYDRGPIQWMACENKGYYYEIPSIGAIR
INTQEYLDVLGRPMVLAGDKAKQVQWTNVYLDALELGLVITGTLPVFNITGQFENKTNLKNQLILGVMGV
DVSLEDIKRLTPRFTLCPNGYYFAIDPNGYVLLHPNLQPKNPKSQEPVTLDFLDAELENDIKVEIRNKMI
DGESGEKTFRTLVKSQDERYIDKGNRTYTWTPVNGTDYSLALVLPTYSFYYIKAKLEETITQARSKKGKM
KDSETLKPDNFEESGYTFIAPRDYCNDLKISDNNTEFLLNFNEFIDRKTPNNPSCNADLINRVLLDAGFT
NELVQNYWSKQKNIKGVKARFVVTDGGITRVYPKEAGENWQENPETYEDSFYKRSLDNDNYVFTAPYFNK
SGPGAYESGIMVSKAVEIYIQGKLLKPAVVGIKIDVNSWIENFTKTSIRDPCAGPVCDCKRNSDVMDCVI
LDDGGFLLMANHDDYTNQIGRFFGEIDPSLMRHLVNISVYAFNKSYDYQSVCEPGAAPKQGAGHRSAYVP
SVADILQIGWWATAAAWSILQQFLLSLTFPRLLEAVEMEDDDFTASLSKQSCITEQTQYFFDNDSKSFSG
VLDCGNCSRIFHGEKLMNTNLIFIMVESKGTCPCDTRLLIQAEQTSDGPNPCDMVKQPRYRKGPDVCFDN
NVLEDYTDCGGVSGLNPSLWYIIGIQFLLLWLVSGSTHRLL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_000722
ORF Size 3273 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_000722.4
RefSeq Size 3822 bp
RefSeq ORF 3276 bp
Locus ID 781
UniProt ID P54289
Cytogenetics 7q21.11
Domains VWA, Cache
Protein Families Druggable Genome, Ion Channels: Other
Protein Pathways Arrhythmogenic right ventricular cardiomyopathy (ARVC), Cardiac muscle contraction, Dilated cardiomyopathy, Hypertrophic cardiomyopathy (HCM), MAPK signaling pathway
MW 123 kDa
Gene Summary The preproprotein encoded by this gene is cleaved into multiple chains that comprise the alpha-2 and delta subunits of the voltage-dependent calcium channel complex. Calcium channels mediate the influx of calcium ions into the cell upon membrane polarization. Mutations in this gene can cause cardiac deficiencies, including Brugada syndrome and short QT syndrome. Alternate splicing results in multiple transcript variants, some of which may lack the delta subunit portion. [provided by RefSeq, Nov 2014]

Other Versions

Other products for "CACNA2D1"
Frequently bought together (5)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 30 ul

USD 165.00


pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

USD 627.00


Rabbit Polyclonal Anti-CACNA2D1 Antibody
    • 100 ul

USD 475.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

USD 149.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

USD 55.00

Customer Reviews 
Loading...
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.