CSB (ERCC6) (NM_000124) Human Tagged Lenti ORF Clone

CAT#: RC219020L1

  • LentiORF®

Lenti ORF clone of Human excision repair cross-complementing rodent repair deficiency, complementation group 6 (ERCC6), Myc-DDK-tagged


  "NM_000124" in other vectors (6)

Reconstitution Protocol

USD 2,215.00

In Stock*

Size
    • 10 ug

Product Images

Frequently bought together (5)
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Reverse primer to sequence targets cloned in expression vectors.
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Rabbit polyclonal antibody to CSB (excision repair cross-complementing rodent repair deficiency, complementation group 6)
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DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol CSB
Synonyms ARMD5; CKN2; COFS; COFS1; CSB; CSB-PGBD3; POF11; RAD26; UVSS1
Vector pLenti-C-Myc-DDK
E. coli Selection Chloramphenicol (34 ug/mL)
Mammalian Cell Selection None
Sequence Data
The ORF insert of this clone is exactly the same as(RC219020).
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_000124
ORF Size 4479 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_000124.1
RefSeq Size 8993 bp
RefSeq ORF 4482 bp
Locus ID 2074
UniProt ID Q03468
Cytogenetics 10q11.23
Domains SNF2_N, DEAD, helicase_C
Protein Families Druggable Genome
Protein Pathways Nucleotide excision repair
MW 168.3 kDa
Gene Summary This gene encodes a DNA-binding protein that is important in transcription-coupled excision repair. The encoded protein has ATP-stimulated ATPase activity, interacts with several transcription and excision repair proteins, and may promote complex formation at DNA repair sites. Mutations in this gene are associated with Cockayne syndrome type B and cerebrooculofacioskeletal syndrome 1. Alternative splicing occurs between a splice site from exon 5 of this gene to the 3' splice site upstream of the open reading frame (ORF) of the adjacent gene, piggyback-derived-3 (GeneID:267004), which activates the alternative polyadenylation site downstream of the piggyback-derived-3 ORF. The resulting transcripts encode a fusion protein that shares sequence with the product of each individual gene. [provided by RefSeq, Mar 2016]

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.