GAB1 (NM_002039) Human Tagged ORF Clone

CAT#: RC219005

GAB1 (Myc-DDK-tagged)-Human GRB2-associated binding protein 1 (GAB1), transcript variant 2

Product Images

Circular map for RC219005

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  Western blot validation of overexpression lysate (Cat# LY400746) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC219005 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified GAB1 protein (Cat# TP319005). The protein was produced from HEK293T cells transfected with GAB1 cDNA clone (Cat# RC219005) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GAB1
• Synonyms: DFNB26
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC219005 representing NM_002039
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCGGTGGTGAAGTGGTCTGCTCCGGATGGCTCCGCAAGTCCCCCCCGGAGAAAAAGTTGAAGCGTT
  ATGCATGGAAGAGGAGATGGTTCGTGTTACGCAGTGGCCGTTTAACTGGAGATCCAGATGTTTTGGAATA
  TTACAAAAATGATCATGCCAAGAAGCCTATTCGTATTATTGATTTAAATTTATGTCAACAAGTAGATGCT
  GGATTGACATTTAACAAAAAAGAGTTTGAAAACAGCTACATTTTTGATATCAACACTATTGACCGGATTT
  TCTACTTGGTAGCAGACAGCGAGGAGGAGATGAATAAGTGGGTTCGTTGTATTTGTGACATCTGTGGGTT
  TAATCCAACAGAAGAAGATCCTGTGAAGCCACCTGGCAGCTCTTTACAAGCACCAGCTGATTTACCTTTA
  GCTATAAATACAGCACCACCATCCACCCAGGCAGATTCATCCTCTGCTACTCTACCTCCTCCATATCAGC
  TAATCAATGTTCCACCACACCTGGAAACTCTTGGCATTCAGGAGGATCCTCAAGACTACCTGTTGCTCAT
  CAACTGTCAAAGCAAGAAGCCCGAACCCACCAGAACGCATGCTGATTCTGCAAAATCCACCTCTTCTGAA
  ACAGACTGCAATGATAACGTCCCTTCTCATAAAAATCCTGCTTCCTCCCAGAGCAAACATGGAATGAATG
  GCTTTTTTCAGCAGCAAATGATATACGACTCTCCACCTTCACGTGCCCCATCTGCTTCAGTTGACTCCAG
  CCTTTATAACCTGCCCAGGAGTTATTCCCATGATGTTTTACCAAAGGTGTCTCCATCAAGTACTGAAGCA
  GATGGAGAACTCTATGTTTTTAATACCCCATCTGGGACATCGAGTGTAGAGACTCAAATGAGGCATGTAT
  CTATTAGTTATGACATTCCTCCAACACCTGGTAATACTTATCAGATTCCACGAACATTTCCAGAAGGAAC
  CTTGGGACAGACATCAAAGCTAGACACTATTCCAGATATTCCTCCACCTCGGCCACCGAAACCACATCCA
  GCTCATGACCGATCTCCTGTGGAAACGTGTAGTATCCCACGCACCGCCTCAGACACTGACAGTAGTTACT
  GTATCCCTACAGCAGGGATGTCGCCTTCACGTAGTAATACCATTTCCACTGTGGATTTAAACAAATTGCG
  AAAAGATGCTAGTTCTCAAGACTGCTATGATATTCCACGAGCATTTCCAAGTGATAGATCTAGTTCACTT
  GAAGGCTTCCATAACCACTTTAAAGTCAAAAATGTGTTGACAGTGGGAAGTGTTTCAAGTGAAGAACTGG
  ATGAAAATTACGTCCCAATGAATCCCAATTCACCACCACGACAACATTCCAGCAGTTTTACAGAACCAAT
  TCAGGAAGCAAATTATGTGCCAATGACTCCAGGAACATTTGATTTTTCCTCATTTGGAATGCAAGTTCCT
  CCTCCTGCTCATATGGGCTTCAGGTCCAGCCCAAAAACCCCTCCCAGAAGGCCAGTTCCTGTTGCAGACT
  GTGAACCACCCCCCGTGGATAGGAACCTCAAGCCAGACAGAAAAGTCAAGCCAGCGCCTTTAGAAATAAA
  ACCTTTGCCAGAATGGGAAGAATTACAAGCCCCAGTTAGATCTCCCATCACTAGGAGTTTTGCTCGAGAC
  TCTTCCAGGTTTCCCATGTCCCCCCGACCAGATTCAGTGCATAGCACAACTTCAAGCAGTGACTCACACG
  ACAGTGAAGAGAATTATGTTCCCATGAACCCAAACCTGTCCAGTGAAGACCCAAATCTCTTTGGCAGTAA
  CAGTCTTGATGGAGGAAGCAGCCCTATGATCAAGCCCAAAGGAGACAAACAGGTGGAATACTTAGATCTC
  GACTTAGATTCTGGGAAATCCACACCACCACGTAAGCAAAAGAGCAGTGGCTCAGGCAGCAGTGTAGCAG
  ATGAGAGAGTGGATTATGTTGTTGTTGACCAACAGAAGACCTTGGCTCTAAAGAGTACCCGGGAAGCCTG
  GACAGATGGGAGACAGTCCACAGAATCAGAAACGCCAGCGAAGAGTGTGAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC219005 representing NM_002039
  Red=Cloning site Green=Tags(s)
  
  MSGGEVVCSGWLRKSPPEKKLKRYAWKRRWFVLRSGRLTGDPDVLEYYKNDHAKKPIRIIDLNLCQQVDA
  GLTFNKKEFENSYIFDINTIDRIFYLVADSEEEMNKWVRCICDICGFNPTEEDPVKPPGSSLQAPADLPL
  AINTAPPSTQADSSSATLPPPYQLINVPPHLETLGIQEDPQDYLLLINCQSKKPEPTRTHADSAKSTSSE
  TDCNDNVPSHKNPASSQSKHGMNGFFQQQMIYDSPPSRAPSASVDSSLYNLPRSYSHDVLPKVSPSSTEA
  DGELYVFNTPSGTSSVETQMRHVSISYDIPPTPGNTYQIPRTFPEGTLGQTSKLDTIPDIPPPRPPKPHP
  AHDRSPVETCSIPRTASDTDSSYCIPTAGMSPSRSNTISTVDLNKLRKDASSQDCYDIPRAFPSDRSSSL
  EGFHNHFKVKNVLTVGSVSSEELDENYVPMNPNSPPRQHSSSFTEPIQEANYVPMTPGTFDFSSFGMQVP
  PPAHMGFRSSPKTPPRRPVPVADCEPPPVDRNLKPDRKVKPAPLEIKPLPEWEELQAPVRSPITRSFARD
  SSRFPMSPRPDSVHSTTSSSDSHDSEENYVPMNPNLSSEDPNLFGSNSLDGGSSPMIKPKGDKQVEYLDL
  DLDSGKSTPPRKQKSSGSGSSVADERVDYVVVDQQKTLALKSTREAWTDGRQSTESETPAKSVK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002039
• ORF Size: 2082 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002039.4
• RefSeq Size: 3323 bp
• RefSeq ORF: 2085 bp
• Locus ID: 2549
• UniProt ID: Q13480
• Cytogenetics: 4q31.21
• Domains: PH
• Protein Families: Druggable Genome
• Protein Pathways: ErbB signaling pathway, Neurotrophin signaling pathway, Renal cell carcinoma
• MW: 76.4 kDa
• Gene Summary: The protein encoded by this gene is a member of the IRS1-like multisubstrate docking protein family. It is an important mediator of branching tubulogenesis and plays a central role in cellular growth response, transformation and apoptosis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]