OTUD3 (NM_015207) Human Tagged ORF Clone

CAT#: RC218984

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OTUD3 (Myc-DDK-tagged)-Human OTU domain containing 3 (OTUD3)



  "NM_015207" in other vectors (6)

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 477.00


Availability*
In Stock

Size
    • 10 ug


Product images

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol OTUD3
Synonyms DUBA4
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC218984 representing NM_015207
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCCCGAAAGCAGGCGGCGAAGAGCCGGCCGGGCAGCGGCAGCCGGAAAGCCGAGGCCGAGCGCAAGC
GGGACGAGCGGGCGGCGCGCCGGGCCCTGGCCAAGGAGCGGCGGAATCGGCCGGAGTCTGGCGGCGGCGG
CGGCTGCGAGGAGGAGTTCGTCAGCTTCGCCAACCAGCTGCAGGCCCTGGGGCTGAAGCTGCGGGAGGTG
CCGGGGGACGGCAATTGCTTGTTCAGAGCTCTTGGTGATCAATTGGAGGGACACTCACGAAATCATCTCA
AGCACAGACAGGAGACAGTGGACTACATGATAAAGCAGCGGGAAGATTTTGAACCCTTTGTAGAAGATGA
CATTCCTTTTGAGAAGCATGTGGCCAGTTTGGCAAAGCCTGGTACTTTTGCTGGCAATGATGCAATTGTA
GCCTTTGCAAGAAATCATCAGTTGAATGTAGTGATTCATCAACTTAATGCCCCTTTGTGGCAGATTCGTG
GTACAGAGAAAAGCAGCGTGAGGGAGTTACACATCGCATATCGGTATGGAGAGCACTACGACAGTGTTCG
GAGGATCAATGACAACTCAGAGGCACCTGCACATCTCCAGACGGATTTTCAGATGCTTCATCAAGATGAA
TCAAATAAAAGAGAAAAGATCAAGACAAAGGGAATGGACTCTGAAGACGACCTGAGAGATGAAGTAGAGG
ATGCTGTCCAGAAAGTTTGTAATGCAACTGGATGTTCAGATTTTAATTTAATAGTCCAGAACCTGGAAGC
TGAAAATTATAATATTGAATCTGCAATAATTGCCGTGCTTCGGATGAACCAAGGGAAGAGAAATAATGCA
GAAGAGAATCTTGAGCCCAGTGGTCGAGTGCTGAAGCAGTGTGGCCCTTTGTGGGAGGAGGGTGGCAGTG
GTGCCAGAATCTTTGGAAATCAGGGCTTAAATGAAGGCAGGACCGAAAACAATAAGGCACAGGCCAGCCC
TAGTGAAGAAAACAAAGCAAATAAAAACCAGCTCGCAAAGGTCACAAACAAACAGAGGCGAGAACAGCAG
TGGATGGAGAAGAAGAAGCGGCAGGAGGAGAGGCACCGCCACAAAGCCCTGGAGAGCAGAGGTAGCCACA
GGGACAATAACAGAAGCGAAGCAGAGGCGAACACGCAGGTCACCTTGGTGAAGACCTTCGCCGCTCTCAA
CATC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC218984 representing NM_015207
Red=Cloning site Green=Tags(s)

MSRKQAAKSRPGSGSRKAEAERKRDERAARRALAKERRNRPESGGGGGCEEEFVSFANQLQALGLKLREV
PGDGNCLFRALGDQLEGHSRNHLKHRQETVDYMIKQREDFEPFVEDDIPFEKHVASLAKPGTFAGNDAIV
AFARNHQLNVVIHQLNAPLWQIRGTEKSSVRELHIAYRYGEHYDSVRRINDNSEAPAHLQTDFQMLHQDE
SNKREKIKTKGMDSEDDLRDEVEDAVQKVCNATGCSDFNLIVQNLEAENYNIESAIIAVLRMNQGKRNNA
EENLEPSGRVLKQCGPLWEEGGSGARIFGNQGLNEGRTENNKAQASPSEENKANKNQLAKVTNKQRREQQ
WMEKKKRQEERHRHKALESRGSHRDNNRSEAEANTQVTLVKTFAALNI

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_015207
ORF Size 1194 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_015207.2
RefSeq Size 6523 bp
RefSeq ORF 1197 bp
Locus ID 23252
UniProt ID Q5T2D3
Cytogenetics 1p36.13
MW 44.9 kDa
Gene Summary Deubiquitinating enzyme that hydrolyzes 'Lys-6'- and 'Lys-11'-linked polyubiquitin. Also hydrolyzes heterotypic (mixed and branched) and homotypic chains.[UniProtKB/Swiss-Prot Function]

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