OTUD3 (NM_015207) Human Tagged ORF Clone

CAT#: RC218984

OTUD3 (Myc-DDK-tagged)-Human OTU domain containing 3 (OTUD3)

Product Images

Western blot validation of overexpression lysate (Cat# LY414729) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC218984 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified OTUD3 protein (Cat# TP318984). The protein was produced from HEK293T cells transfected with OTUD3 cDNA clone (Cat# RC218984) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: OTUD3
• Synonyms: DUBA4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC218984 representing NM_015207
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCCGAAAGCAGGCGGCGAAGAGCCGGCCGGGCAGCGGCAGCCGGAAAGCCGAGGCCGAGCGCAAGC
  GGGACGAGCGGGCGGCGCGCCGGGCCCTGGCCAAGGAGCGGCGGAATCGGCCGGAGTCTGGCGGCGGCGG
  CGGCTGCGAGGAGGAGTTCGTCAGCTTCGCCAACCAGCTGCAGGCCCTGGGGCTGAAGCTGCGGGAGGTG
  CCGGGGGACGGCAATTGCTTGTTCAGAGCTCTTGGTGATCAATTGGAGGGACACTCACGAAATCATCTCA
  AGCACAGACAGGAGACAGTGGACTACATGATAAAGCAGCGGGAAGATTTTGAACCCTTTGTAGAAGATGA
  CATTCCTTTTGAGAAGCATGTGGCCAGTTTGGCAAAGCCTGGTACTTTTGCTGGCAATGATGCAATTGTA
  GCCTTTGCAAGAAATCATCAGTTGAATGTAGTGATTCATCAACTTAATGCCCCTTTGTGGCAGATTCGTG
  GTACAGAGAAAAGCAGCGTGAGGGAGTTACACATCGCATATCGGTATGGAGAGCACTACGACAGTGTTCG
  GAGGATCAATGACAACTCAGAGGCACCTGCACATCTCCAGACGGATTTTCAGATGCTTCATCAAGATGAA
  TCAAATAAAAGAGAAAAGATCAAGACAAAGGGAATGGACTCTGAAGACGACCTGAGAGATGAAGTAGAGG
  ATGCTGTCCAGAAAGTTTGTAATGCAACTGGATGTTCAGATTTTAATTTAATAGTCCAGAACCTGGAAGC
  TGAAAATTATAATATTGAATCTGCAATAATTGCCGTGCTTCGGATGAACCAAGGGAAGAGAAATAATGCA
  GAAGAGAATCTTGAGCCCAGTGGTCGAGTGCTGAAGCAGTGTGGCCCTTTGTGGGAGGAGGGTGGCAGTG
  GTGCCAGAATCTTTGGAAATCAGGGCTTAAATGAAGGCAGGACCGAAAACAATAAGGCACAGGCCAGCCC
  TAGTGAAGAAAACAAAGCAAATAAAAACCAGCTCGCAAAGGTCACAAACAAACAGAGGCGAGAACAGCAG
  TGGATGGAGAAGAAGAAGCGGCAGGAGGAGAGGCACCGCCACAAAGCCCTGGAGAGCAGAGGTAGCCACA
  GGGACAATAACAGAAGCGAAGCAGAGGCGAACACGCAGGTCACCTTGGTGAAGACCTTCGCCGCTCTCAA
  CATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC218984 representing NM_015207
  Red=Cloning site Green=Tags(s)
  
  MSRKQAAKSRPGSGSRKAEAERKRDERAARRALAKERRNRPESGGGGGCEEEFVSFANQLQALGLKLREV
  PGDGNCLFRALGDQLEGHSRNHLKHRQETVDYMIKQREDFEPFVEDDIPFEKHVASLAKPGTFAGNDAIV
  AFARNHQLNVVIHQLNAPLWQIRGTEKSSVRELHIAYRYGEHYDSVRRINDNSEAPAHLQTDFQMLHQDE
  SNKREKIKTKGMDSEDDLRDEVEDAVQKVCNATGCSDFNLIVQNLEAENYNIESAIIAVLRMNQGKRNNA
  EENLEPSGRVLKQCGPLWEEGGSGARIFGNQGLNEGRTENNKAQASPSEENKANKNQLAKVTNKQRREQQ
  WMEKKKRQEERHRHKALESRGSHRDNNRSEAEANTQVTLVKTFAALNI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015207
• ORF Size: 1194 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015207.2
• RefSeq Size: 6523 bp
• RefSeq ORF: 1197 bp
• Locus ID: 23252
• UniProt ID: Q5T2D3
• Cytogenetics: 1p36.13
• MW: 44.9 kDa
• Gene Summary: Deubiquitinating enzyme that hydrolyzes 'Lys-6'- and 'Lys-11'-linked polyubiquitin. Also hydrolyzes heterotypic (mixed and branched) and homotypic chains.[UniProtKB/Swiss-Prot Function]