MRP2 (ABCC2) (NM_000392) Human Tagged ORF Clone

CAT#: RC218976

ABCC2 (Myc-DDK-tagged)-Human ATP-binding cassette, sub-family C (CFTR/MRP), member 2 (ABCC2)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY ABCC2 (Cat# RC218976, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ABCC2 (Cat# TA812520)(1:500).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ABCC2
• Synonyms: ABC30; CMOAT; cMRP; DJS; MRP2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC218976 representing NM_000392
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGGAGAAGTTCTGCAACTCTACTTTTTGGAATTCCTCATTCCTGGACAGTCCGGAGGCAGACCTGC
  CACTTTGTTTTGAGCAAACTGTTCTGGTGTGGATTCCCTTGGGCTTCCTATGGCTCCTGGCCCCCTGGCA
  GCTTCTCCACGTGTATAAATCCAGGACCAAGAGATCCTCTACCACCAAACTCTATCTTGCTAAGCAGGTA
  TTCGTTGGTTTTCTTCTTATTCTAGCAGCCATAGAGCTGGCCCTTGTACTCACAGAAGACTCTGGACAAG
  CCACAGTCCCTGCTGTTCGATATACCAATCCAAGCCTCTACCTAGGCACATGGCTCCTGGTTTTGCTGAT
  CCAATACAGCAGACAATGGTGTGTACAGAAAAACTCCTGGTTCCTGTCCCTATTCTGGATTCTCTCGATA
  CTCTGTGGCACTTTCCAATTTCAGACTCTGATCCGGACACTCTTACAGGGTGACAATTCTAATCTAGCCT
  ACTCCTGCCTGTTCTTCATCTCCTACGGATTCCAGATCCTGATCCTGATCTTTTCAGCATTTTCAGAAAA
  TAATGAGTCATCAAATAATCCATCATCCATAGCTTCATTCCTGAGTAGCATTACCTACAGCTGGTATGAC
  AGCATCATTCTGAAAGGCTACAAGCGTCCTCTGACACTCGAGGATGTCTGGGAAGTTGATGAAGAGATGA
  AAACCAAGACATTAGTGAGCAAGTTTGAAACGCACATGAAGAGAGAGCTGCAGAAAGCCAGGCGGGCACT
  CCAGAGACGGCAGGAGAAGAGCTCCCAGCAGAACTCTGGAGCCAGGCTGCCTGGCTTGAACAAGAATCAG
  AGTCAAAGCCAAGATGCCCTTGTCCTGGAAGATGTTGAAAAGAAAAAAAAGAAGTCTGGGACCAAAAAAG
  ATGTTCCAAAATCCTGGTTGATGAAGGCTCTGTTCAAAACTTTCTACATGGTGCTCCTGAAATCATTCCT
  ACTGAAGCTAGTGAATGACATCTTCACGTTTGTGAGTCCTCAGCTGCTGAAATTGCTGATCTCCTTTGCA
  AGTGACCGTGACACATATTTGTGGATTGGATATCTCTGTGCAATCCTCTTATTCACTGCGGCTCTCATTC
  AGTCTTTCTGCCTTCAGTGTTATTTCCAACTGTGCTTCAAGCTGGGTGTAAAAGTACGGACAGCTATCAT
  GGCTTCTGTATATAAGAAGGCATTGACCCTATCCAACTTGGCCAGGAAGGAGTACACCGTTGGAGAAACA
  GTGAACCTGATGTCTGTGGATGCCCAGAAGCTCATGGATGTGACCAACTTCATGCACATGCTGTGGTCAA
  GTGTTCTACAGATTGTCTTATCTATCTTCTTCCTATGGAGAGAGTTGGGACCCTCAGTCTTAGCAGGTGT
  TGGGGTGATGGTGCTTGTAATCCCAATTAATGCGATACTGTCCACCAAGAGTAAGACCATTCAGGTCAAA
  AATATGAAGAATAAAGACAAACGTTTAAAGATCATGAATGAGATTCTTAGTGGAATCAAGATCCTGAAAT
  ATTTTGCCTGGGAACCTTCATTCAGAGACCAAGTACAAAACCTCCGGAAGAAAGAGCTCAAGAACCTGCT
  GGCCTTTAGTCAACTACAGTGTGTAGTAATATTCGTCTTCCAGTTAACTCCAGTCCTGGTATCTGTGGTC
  ACATTTTCTGTTTATGTCCTGGTGGATAGCAACAATATTTTGGATGCACAAAAGGCCTTCACCTCCATTA
  CCCTCTTCAATATCCTGCGCTTTCCCCTGAGCATGCTTCCCATGATGATCTCCTCCATGCTCCAGGCCAG
  TGTTTCCACAGAGCGGCTAGAGAAGTACTTGGGAGGGGATGACTTGGACACATCTGCCATTCGACATGAC
  TGCAATTTTGACAAAGCCATGCAGTTTTCTGAGGCCTCCTTTACCTGGGAACATGATTCGGAAGCCACAG
  TCCGAGATGTGAACCTGGACATTATGGCAGGCCAACTTGTGGCTGTGATAGGCCCTGTCGGCTCTGGGAA
  ATCCTCCTTGATATCAGCCATGCTGGGAGAAATGGAAAATGTCCACGGGCACATCACCATCAAGGGCACC
  ACTGCCTATGTCCCACAGCAGTCCTGGATTCAGAATGGCACCATAAAGGACAACATCCTTTTTGGAACAG
  AGTTTAATGAAAAGAGGTACCAGCAAGTACTGGAGGCCTGTGCTCTCCTCCCAGACTTGGAAATGCTGCC
  TGGAGGAGATTTGGCTGAGATTGGAGAGAAGGGTATAAATCTTAGTGGGGGTCAGAAGCAGCGGATCAGC
  CTGGCCAGAGCTACCTACCAAAATTTAGACATCTATCTTCTAGATGACCCCCTGTCTGCAGTGGATGCTC
  ATGTAGGAAAACATATTTTTAATAAGGTCTTGGGCCCCAATGGCCTGTTGAAAGGCAAGACTCGACTCTT
  GGTTACACATAGCATGCACTTTCTTCCTCAAGTGGATGAGATTGTAGTTCTGGGGAATGGAACAATTGTA
  GAGAAAGGATCCTACAGTGCTCTCCTGGCCAAAAAAGGAGAGTTTGCTAAGAATCTGAAGACATTTCTAA
  GACATACAGGCCCTGAAGAGGAAGCCACAGTCCATGATGGCAGTGAAGAAGAAGACGATGACTATGGGCT
  GATATCCAGTGTGGAAGAGATCCCCGAAGATGCAGCCTCCATAACCATGAGAAGAGAGAACAGCTTTCGT
  CGAACACTTAGCCGCAGTTCTAGGTCCAATGGCAGGCATCTGAAGTCCCTGAGAAACTCCTTGAAAACTC
  GGAATGTGAATAGCCTGAAGGAAGACGAAGAACTAGTGAAAGGACAAAAACTAATTAAGAAGGAATTCAT
  AGAAACTGGAAAGGTGAAGTTCTCCATCTACCTGGAGTACCTACAAGCAATAGGATTGTTTTCGATATTC
  TTCATCATCCTTGCGTTTGTGATGAATTCTGTGGCTTTTATTGGATCCAACCTCTGGCTCAGTGCTTGGA
  CCAGTGACTCTAAAATCTTCAATAGCACCGACTATCCAGCATCTCAGAGGGACATGAGAGTTGGAGTCTA
  CGGAGCTCTGGGATTAGCCCAAGGTATATTTGTGTTCATAGCACATTTCTGGAGTGCCTTTGGTTTCGTC
  CATGCATCAAATATCTTGCACAAGCAACTGCTGAACAATATCCTTCGAGCACCTATGAGATTTTTTGACA
  CAACACCCACAGGCCGGATTGTGAACAGGTTTGCCGGCGATATTTCCACAGTGGATGACACCCTGCCTCA
  GTCCTTGCGCAGCTGGATTACATGCTTCCTGGGGATAATCAGCACCCTTGTCATGATCTGCATGGCCACT
  CCTGTCTTCACCATCATCGTCATTCCTCTTGGCATTATTTATGTATCTGTTCAGATGTTTTATGTGTCTA
  CCTCCCGCCAGCTGAGGCGTCTGGACTCTGTCACCAGGTCCCCAATCTACTCTCACTTCAGCGAGACCGT
  ATCAGGTTTGCCAGTTATCCGTGCCTTTGAGCACCAGCAGCGATTTCTGAAACACAATGAGGTGAGGATT
  GACACCAACCAGAAATGTGTCTTTTCCTGGATCACCTCCAACAGGTGGCTTGCAATTCGCCTGGAGCTGG
  TTGGGAACCTGACTGTCTTCTTTTCAGCCTTGATGATGGTTATTTATAGAGATACCCTAAGTGGGGACAC
  TGTTGGCTTTGTTCTGTCCAATGCACTCAATATCACACAAACCCTGAACTGGCTGGTGAGGATGACATCA
  GAAATAGAGACCAACATTGTGGCTGTTGAGCGAATAACTGAGTACACAAAAGTGGAAAATGAGGCACCCT
  GGGTGACTGATAAGAGGCCTCCGCCAGATTGGCCCAGCAAAGGCAAGATCCAGTTTAACAACTACCAAGT
  GCGGTACCGACCTGAGCTGGATCTGGTCCTCAGAGGGATCACTTGTGACATCGGTAGCATGGAGAAGATT
  GGTGTGGTGGGCAGGACAGGAGCTGGAAAGTCATCCCTCACAAACTGCCTCTTCAGAATCTTAGAGGCTG
  CCGGTGGTCAGATTATCATTGATGGAGTAGATATTGCTTCCATTGGGCTCCACGACCTCCGAGAGAAGCT
  GACCATCATCCCCCAGGACCCCATCCTGTTCTCTGGAAGCCTGAGGATGAATCTCGACCCTTTCAACAAC
  TACTCAGATGAGGAGATTTGGAAGGCCTTGGAGCTGGCTCACCTCAAGTCTTTTGTGGCCAGCCTGCAAC
  TTGGGTTATCCCACGAAGTGACAGAGGCTGGTGGCAACCTGAGCATAGGCCAGAGGCAGCTGCTGTGCCT
  GGGCAGGGCTCTGCTTCGGAAATCCAAGATCCTGGTCCTGGATGAGGCCACTGCTGCGGTGGATCTAGAG
  ACAGACAACCTCATTCAGACGACCATCCAAAACGAGTTCGCCCACTGCACAGTGATCACCATCGCCCACA
  GGCTGCACACCATCATGGACAGTGACAAGGTAATGGTCCTAGACAACGGGAAGATTATAGAGTGCGGCAG
  CCCTGAAGAACTGCTACAAATCCCTGGACCCTTTTACTTTATGGCTAAGGAAGCTGGCATTGAGAATGTG
  AACAGCACAAAATTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC218976 representing NM_000392
  Red=Cloning site Green=Tags(s)
  
  MLEKFCNSTFWNSSFLDSPEADLPLCFEQTVLVWIPLGFLWLLAPWQLLHVYKSRTKRSSTTKLYLAKQV
  FVGFLLILAAIELALVLTEDSGQATVPAVRYTNPSLYLGTWLLVLLIQYSRQWCVQKNSWFLSLFWILSI
  LCGTFQFQTLIRTLLQGDNSNLAYSCLFFISYGFQILILIFSAFSENNESSNNPSSIASFLSSITYSWYD
  SIILKGYKRPLTLEDVWEVDEEMKTKTLVSKFETHMKRELQKARRALQRRQEKSSQQNSGARLPGLNKNQ
  SQSQDALVLEDVEKKKKKSGTKKDVPKSWLMKALFKTFYMVLLKSFLLKLVNDIFTFVSPQLLKLLISFA
  SDRDTYLWIGYLCAILLFTAALIQSFCLQCYFQLCFKLGVKVRTAIMASVYKKALTLSNLARKEYTVGET
  VNLMSVDAQKLMDVTNFMHMLWSSVLQIVLSIFFLWRELGPSVLAGVGVMVLVIPINAILSTKSKTIQVK
  NMKNKDKRLKIMNEILSGIKILKYFAWEPSFRDQVQNLRKKELKNLLAFSQLQCVVIFVFQLTPVLVSVV
  TFSVYVLVDSNNILDAQKAFTSITLFNILRFPLSMLPMMISSMLQASVSTERLEKYLGGDDLDTSAIRHD
  CNFDKAMQFSEASFTWEHDSEATVRDVNLDIMAGQLVAVIGPVGSGKSSLISAMLGEMENVHGHITIKGT
  TAYVPQQSWIQNGTIKDNILFGTEFNEKRYQQVLEACALLPDLEMLPGGDLAEIGEKGINLSGGQKQRIS
  LARATYQNLDIYLLDDPLSAVDAHVGKHIFNKVLGPNGLLKGKTRLLVTHSMHFLPQVDEIVVLGNGTIV
  EKGSYSALLAKKGEFAKNLKTFLRHTGPEEEATVHDGSEEEDDDYGLISSVEEIPEDAASITMRRENSFR
  RTLSRSSRSNGRHLKSLRNSLKTRNVNSLKEDEELVKGQKLIKKEFIETGKVKFSIYLEYLQAIGLFSIF
  FIILAFVMNSVAFIGSNLWLSAWTSDSKIFNSTDYPASQRDMRVGVYGALGLAQGIFVFIAHFWSAFGFV
  HASNILHKQLLNNILRAPMRFFDTTPTGRIVNRFAGDISTVDDTLPQSLRSWITCFLGIISTLVMICMAT
  PVFTIIVIPLGIIYVSVQMFYVSTSRQLRRLDSVTRSPIYSHFSETVSGLPVIRAFEHQQRFLKHNEVRI
  DTNQKCVFSWITSNRWLAIRLELVGNLTVFFSALMMVIYRDTLSGDTVGFVLSNALNITQTLNWLVRMTS
  EIETNIVAVERITEYTKVENEAPWVTDKRPPPDWPSKGKIQFNNYQVRYRPELDLVLRGITCDIGSMEKI
  GVVGRTGAGKSSLTNCLFRILEAAGGQIIIDGVDIASIGLHDLREKLTIIPQDPILFSGSLRMNLDPFNN
  YSDEEIWKALELAHLKSFVASLQLGLSHEVTEAGGNLSIGQRQLLCLGRALLRKSKILVLDEATAAVDLE
  TDNLIQTTIQNEFAHCTVITIAHRLHTIMDSDKVMVLDNGKIIECGSPEELLQIPGPFYFMAKEAGIENV
  NSTKF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_000392
• ORF Size: 4635 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000392.4
• RefSeq Size: 4868 bp
• RefSeq ORF: 4638 bp
• Locus ID: 1244
• UniProt ID: Q92887
• Cytogenetics: 10q24.2
• Domains: ABC_membrane, ABC_tran, AAA
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: ABC transporters
• MW: 174 kDa
• Gene Summary: The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MRP subfamily which is involved in multi-drug resistance. This protein is expressed in the canalicular (apical) part of the hepatocyte and functions in biliary transport. Substrates include anticancer drugs such as vinblastine; therefore, this protein appears to contribute to drug resistance in mammalian cells. Several different mutations in this gene have been observed in patients with Dubin-Johnson syndrome (DJS), an autosomal recessive disorder characterized by conjugated hyperbilirubinemia. [provided by RefSeq, Jul 2008]