LRP6 (NM_002336) Human Tagged ORF Clone

CAT#: RC218918

LRP6 (Myc-DDK-tagged)-Human low density lipoprotein receptor-related protein 6 (LRP6)

Product Images

Circular map for RC218918

• 

  Western blot validation of overexpression lysate (Cat# LY419396) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC218918 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified LRP6 protein (Cat# TP318918). The protein was produced from HEK293T cells transfected with LRP6 cDNA clone (Cat# RC218918) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: LRP6
• Synonyms: ADCAD2; STHAG7
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC218918 representing NM_002336
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGGCCGTCCTGAGGAGCCTCCTGGCCTGCAGCTTCTGTGTGCTCCTGAGAGCGGCCCCTTTGTTGC
  TTTATGCAAACAGACGGGACTTGCGATTGGTTGATGCTACAAATGGCAAAGAGAATGCTACGATTGTAGT
  TGGAGGCTTGGAGGATGCAGCTGCGGTGGACTTTGTGTTTAGTCATGGCTTGATATACTGGAGTGATGTC
  AGCGAAGAAGCCATTAAACGAACAGAATTTAACAAAACTGAGAGTGTGCAGAATGTTGTTGTTTCTGGAT
  TATTGTCCCCCGATGGGCTGGCATGTGATTGGCTTGGAGAAAAATTGTACTGGACAGATTCTGAAACTAA
  TCGGATTGAAGTTTCTAATTTAGATGGATCTTTACGAAAAGTTTTATTTTGGCAAGAGTTGGATCAACCC
  AGAGCTATTGCCTTAGATCCTTCAAGTGGGTTCATGTACTGGACAGACTGGGGAGAAGTGCCAAAGATAG
  AACGTGCTGGAATGGATGGTTCAAGTCGCTTCATTATAATAAACAGTGAAATTTACTGGCCAAATGGACT
  GACTTTGGATTATGAAGAACAAAAGCTTTATTGGGCAGATGCAAAACTTAATTTCATCCACAAATCAAAT
  CTGGATGGAACAAATCGGCAGGCAGTGGTTAAAGGTTCCCTTCCACATCCTTTTGCCTTGACGTTATTTG
  AGGACATATTGTACTGGACTGACTGGAGCACACACTCCATTTTGGCTTGCAACAAGTATACTGGTGAGGG
  TCTGCGTGAAATCCATTCTGACATCTTCTCTCCCATGGATATACATGCCTTCAGCCAACAGAGGCAGCCA
  AATGCCACAAATCCATGTGGAATTGACAATGGGGGTTGTTCCCATTTGTGTTTGATGTCTCCAGTCAAGC
  CTTTTTATCAGTGTGCTTGCCCCACTGGGGTCAAACTCCTGGAGAATGGAAAAACCTGCAAAGATGGTGC
  CACAGAATTATTGCTTTTAGCTCGAAGGACAGACTTGAGACGCATTTCTTTGGATACACCAGATTTTACA
  GACATTGTTCTGCAGTTAGAAGACATCCGTCATGCCATTGCCATAGATTACGATCCTGTGGAAGGCTACA
  TCTACTGGACTGATGATGAAGTGAGGGCCATACGCCGTTCATTTATAGATGGATCTGGCAGTCAGTTTGT
  GGTCACTGCTCAAATTGCCCATCCTGATGGTATTGCTGTGGACTGGGTTGCACGAAATCTTTATTGGACA
  GACACTGGCACTGATCGAATAGAAGTGACAAGGCTCAATGGGACCATGAGGAAGATCTTGATTTCAGAGG
  ACTTAGAGGAACCCCGGGCTATTGTGTTAGATCCCATGGTTGGGTACATGTATTGGACTGACTGGGGAGA
  AATTCCGAAAATTGAGCGAGCAGCTCTGGATGGTTCTGACCGTGTAGTATTGGTTAACACTTCTCTTGGT
  TGGCCAAATGGTTTAGCCTTGGATTATGATGAAGGCAAAATATACTGGGGAGATGCCAAAACAGACAAGA
  TTGAGGTTATGAATACTGATGGCACTGGGAGACGAGTACTAGTGGAAGACAAAATTCCTCACATATTTGG
  ATTTACTTTGTTGGGTGACTATGTTTACTGGACTGACTGGCAGAGGCGTAGCATTGAAAGAGTTCATAAA
  CGAAGTGCAGAGAGGGAAGTGATCATAGATCAGCTGCCTGACCTCATGGGCCTAAAGGCTACAAATGTTC
  ATCGAGTGATTGGTTCCAACCCCTGTGCTGAGGAAAACGGGGGATGTAGCCATCTCTGCCTCTATAGACC
  TCAGGGCCTTCGCTGTGCTTGCCCTATTGGCTTTGAACTCATCAGTGACATGAAGACCTGCATTGTCCCA
  GAGGCTTTCCTTTTGTTTTCACGGAGAGCAGATATCAGACGAATTTCTCTGGAAACAAACAATAATAATG
  TGGCTATTCCACTCACTGGTGTCAAAGAAGCTTCTGCTTTGGATTTTGATGTGACAGACAACCGAATTTA
  TTGGACTGATATATCACTCAAGACCATCAGCAGAGCCTTTATGAATGGCAGTGCACTGGAACATGTGGTA
  GAATTCGGCTTAGATTATCCAGAAGGCATGGCAGTAGACTGGCTTGGGAAGAACTTGTACTGGGCAGACA
  CAGGAACGAATCGAATTGAGGTGTCAAAGTTGGATGGGCAGCACCGACAAGTTTTGGTGTGGAAAGACCT
  AGATAGTCCCAGAGCTCTCGCGTTGGACCCTGCCGAAGGATTTATGTATTGGACTGAATGGGGTGGAAAA
  CCTAAGATAGACAGAGCTGCAATGGATGGAAGTGAACGTACTACCTTAGTTCCAAATGTGGGGCGGGCAA
  ACGGCCTAACTATTGATTATGCTAAAAGGAGGCTTTATTGGACAGACCTGGACACCAACTTAATAGAATC
  TTCAAATATGCTTGGGCTCAACCGTGAAGTTATAGCAGATGACTTGCCTCATCCTTTTGGCTTAACTCAG
  TACCAAGATTATATCTACTGGACGGACTGGAGCCGACGCAGCATTGAGCGTGCCAACAAAACCAGTGGCC
  AAAACCGCACCATCATTCAGGGCCATTTGGATTATGTGATGGACATCCTCGTCTTTCACTCATCTCGACA
  GTCAGGGTGGAATGAATGTGCTTCCAGCAATGGGCACTGCTCCCACCTCTGCTTGGCTGTGCCAGTTGGG
  GGTTTTGTTTGTGGATGCCCTGCCCACTACTCTCTTAATGCTGACAACAGGACTTGTAGTGCTCCTACGA
  CTTTCCTGCTCTTCAGTCAAAAGAGTGCCATCAACCGCATGGTGATTGATGAACAACAGAGCCCCGACAT
  CATCCTTCCCATCCACAGCCTTCGGAATGTCCGGGCCATTGACTATGACCCACTGGACAAGCAACTCTAT
  TGGATTGACTCACGACAAAACATGATCCGAAAGGCACAAGAAGATGGCAGCCAGGGCTTTACTGTGGTTG
  TGAGCTCAGTTCCGAGTCAGAACCTGGAAATACAACCCTATGACCTCAGCATTGATATTTACAGCCGCTA
  CATCTACTGGACTTGTGAGGCTACCAATGTCATTAATGTGACAAGATTAGATGGGAGATCAGTTGGAGTG
  GTGCTGAAAGGCGAGCAGGACAGACCTCGAGCCATTGTGGTAAACCCAGAGAAAGGGTATATGTATTTTA
  CCAATCTTCAGGAAAGGTCTCCTAAAATTGAACGGGCTGCTTTGGATGGGACAGAACGGGAGGTCCTCTT
  TTTCAGTGGCTTAAGTAAACCAATTGCTTTAGCCCTTGATAGCAGGCTGGGCAAGCTCTTTTGGGCTGAT
  TCAGATCTCCGGCGAATTGAAAGCAGTGATCTCTCAGGTGCTAACCGGATAGTATTAGAAGACTCCAATA
  TCTTGCAGCCTGTGGGACTTACTGTGTTTGAAAACTGGCTCTATTGGATTGATAAACAGCAGCAAATGAT
  TGAAAAAATTGACATGACAGGTCGAGAGGGTAGAACCAAAGTCCAAGCTCGAATTGCCCAGCTTAGTGAC
  ATTCATGCAGTAAAGGAGCTGAACCTTCAAGAATACAGACAGCACCCTTGTGCTCAGGATAATGGTGGCT
  GTTCACATATTTGTCTTGTAAAGGGGGATGGTACTACAAGGTGTTCTTGCCCCATGCACCTGGTTCTACT
  TCAAGATGAGCTATCATGTGGAGAACCTCCAACATGTTCTCCTCAGCAGTTTACTTGTTTCACGGGGGAA
  ATTGACTGTATCCCTGTGGCTTGGCGGTGCGATGGGTTTACTGAATGTGAAGACCACAGTGATGAACTCA
  ATTGTCCTGTATGCTCAGAGTCCCAGTTCCAGTGTGCCAGTGGGCAGTGTATTGATGGTGCCCTCCGATG
  CAATGGAGATGCAAACTGCCAGGACAAATCAGATGAGAAGAACTGTGAAGTGCTTTGTTTAATTGATCAG
  TTCCGCTGTGCCAATGGTCAGTGCATTGGAAAGCACAAGAAGTGTGATCATAATGTGGATTGCAGTGACA
  AGTCAGATGAACTGGATTGTTATCCGACTGAAGAACCAGCACCACAGGCCACCAATACAGTTGGTTCTGT
  TATTGGCGTAATTGTCACCATTTTTGTGTCTGGAACTGTATACTTTATCTGCCAGAGGATGTTGTGTCCA
  CGTATGAAGGGAGATGGGGAAACTATGACTAATGACTATGTAGTTCATGGACCAGCTTCTGTGCCTCTTG
  GTTATGTGCCACACCCAAGTTCTTTGTCAGGATCTCTTCCAGGAATGTCTCGAGGTAAATCAATGATCAG
  CTCCCTCAGTATCATGGGGGGAAGCAGTGGACCCCCCTATGACCGAGCCCATGTTACAGGAGCATCATCA
  AGTAGTTCTTCAAGCACCAAAGGCACTTACTTCCCTGCAATTTTGAACCCTCCACCATCCCCAGCCACAG
  AGCGATCACATTACACTATGGAATTTGGATATTCTTCAAACAGTCCTTCCACTCATAGGTCATACAGCTA
  CAGGCCATATAGCTACCGGCACTTTGCACCCCCCACCACACCCTGCAGCACAGATGTTTGTGACAGTGAC
  TATGCTCCTAGTCGGAGAATGACCTCAGTGGCAACAGCCAAGGGCTATACCAGTGACTTGAACTATGATT
  CAGAACCTGTGCCCCCACCTCCCACACCCCGAAGCCAATACTTGTCAGCAGAGGAGAACTATGAAAGCTG
  CCCACCTTCTCCATACACAGAGAGGAGCTATTCTCATCACCTCTACCCACCGCCACCCTCTCCCTGTACA
  GACTCCTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC218918 representing NM_002336
  Red=Cloning site Green=Tags(s)
  
  MGAVLRSLLACSFCVLLRAAPLLLYANRRDLRLVDATNGKENATIVVGGLEDAAAVDFVFSHGLIYWSDV
  SEEAIKRTEFNKTESVQNVVVSGLLSPDGLACDWLGEKLYWTDSETNRIEVSNLDGSLRKVLFWQELDQP
  RAIALDPSSGFMYWTDWGEVPKIERAGMDGSSRFIIINSEIYWPNGLTLDYEEQKLYWADAKLNFIHKSN
  LDGTNRQAVVKGSLPHPFALTLFEDILYWTDWSTHSILACNKYTGEGLREIHSDIFSPMDIHAFSQQRQP
  NATNPCGIDNGGCSHLCLMSPVKPFYQCACPTGVKLLENGKTCKDGATELLLLARRTDLRRISLDTPDFT
  DIVLQLEDIRHAIAIDYDPVEGYIYWTDDEVRAIRRSFIDGSGSQFVVTAQIAHPDGIAVDWVARNLYWT
  DTGTDRIEVTRLNGTMRKILISEDLEEPRAIVLDPMVGYMYWTDWGEIPKIERAALDGSDRVVLVNTSLG
  WPNGLALDYDEGKIYWGDAKTDKIEVMNTDGTGRRVLVEDKIPHIFGFTLLGDYVYWTDWQRRSIERVHK
  RSAEREVIIDQLPDLMGLKATNVHRVIGSNPCAEENGGCSHLCLYRPQGLRCACPIGFELISDMKTCIVP
  EAFLLFSRRADIRRISLETNNNNVAIPLTGVKEASALDFDVTDNRIYWTDISLKTISRAFMNGSALEHVV
  EFGLDYPEGMAVDWLGKNLYWADTGTNRIEVSKLDGQHRQVLVWKDLDSPRALALDPAEGFMYWTEWGGK
  PKIDRAAMDGSERTTLVPNVGRANGLTIDYAKRRLYWTDLDTNLIESSNMLGLNREVIADDLPHPFGLTQ
  YQDYIYWTDWSRRSIERANKTSGQNRTIIQGHLDYVMDILVFHSSRQSGWNECASSNGHCSHLCLAVPVG
  GFVCGCPAHYSLNADNRTCSAPTTFLLFSQKSAINRMVIDEQQSPDIILPIHSLRNVRAIDYDPLDKQLY
  WIDSRQNMIRKAQEDGSQGFTVVVSSVPSQNLEIQPYDLSIDIYSRYIYWTCEATNVINVTRLDGRSVGV
  VLKGEQDRPRAIVVNPEKGYMYFTNLQERSPKIERAALDGTEREVLFFSGLSKPIALALDSRLGKLFWAD
  SDLRRIESSDLSGANRIVLEDSNILQPVGLTVFENWLYWIDKQQQMIEKIDMTGREGRTKVQARIAQLSD
  IHAVKELNLQEYRQHPCAQDNGGCSHICLVKGDGTTRCSCPMHLVLLQDELSCGEPPTCSPQQFTCFTGE
  IDCIPVAWRCDGFTECEDHSDELNCPVCSESQFQCASGQCIDGALRCNGDANCQDKSDEKNCEVLCLIDQ
  FRCANGQCIGKHKKCDHNVDCSDKSDELDCYPTEEPAPQATNTVGSVIGVIVTIFVSGTVYFICQRMLCP
  RMKGDGETMTNDYVVHGPASVPLGYVPHPSSLSGSLPGMSRGKSMISSLSIMGGSSGPPYDRAHVTGASS
  SSSSSTKGTYFPAILNPPPSPATERSHYTMEFGYSSNSPSTHRSYSYRPYSYRHFAPPTTPCSTDVCDSD
  YAPSRRMTSVATAKGYTSDLNYDSEPVPPPPTPRSQYLSAEENYESCPPSPYTERSYSHHLYPPPPSPCT
  DSS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002336
• ORF Size: 4839 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002336.1, NP_002327.1
• RefSeq Size: 5301 bp
• RefSeq ORF: 4842 bp
• Locus ID: 4040
• UniProt ID: O75581
• Cytogenetics: 12p13.2
• Domains: ldl_recept_b, ldl_recept_a, EGF, EGF
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Wnt signaling pathway
• MW: 180.44 kDa
• Gene Summary: This gene encodes a member of the low density lipoprotein (LDL) receptor gene family. LDL receptors are transmembrane cell surface proteins involved in receptor-mediated endocytosis of lipoprotein and protein ligands. The protein encoded by this gene functions as a receptor or, with Frizzled, a co-receptor for Wnt and thereby transmits the canonical Wnt/beta-catenin signaling cascade. Through its interaction with the Wnt/beta-catenin signaling cascade this gene plays a role in the regulation of cell differentiation, proliferation, and migration and the development of many cancer types. This protein undergoes gamma-secretase dependent RIP- (regulated intramembrane proteolysis) processing but the precise locations of the cleavage sites have not been determined.[provided by RefSeq, Dec 2009]