Eg5 (KIF11) (NM_004523) Human Tagged ORF Clone

CAT#: RC218842

KIF11 (Myc-DDK-tagged)-Human kinesin family member 11 (KIF11)

Product Images

Circular map for RC218842

• 

  Western blot validation of overexpression lysate (Cat# LY401441) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC218842 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified KIF11 protein (Cat# TP318842). The protein was produced from HEK293T cells transfected with KIF11 cDNA clone (Cat# RC218842) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Eg5
• Synonyms: EG5; HKSP; KNSL1; MCLMR; TRIP5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC218842 representing NM_004523
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGTCGCAGCCAAATTCGTCTGCGAAGAAGAAAGAGGAGAAGGGGAAGAACATCCAGGTGGTGGTGA
  GATGCAGACCATTTAATTTGGCAGAGCGGAAAGCTAGCGCCCATTCAATAGTAGAATGTGATCCTGTACG
  AAAAGAAGTTAGTGTACGAACTGGAGGATTGGCTGACAAGAGCTCAAGGAAAACATACACTTTTGATATG
  GTGTTTGGAGCATCTACTAAACAGATTGATGTTTACCGAAGTGTTGTTTGTCCAATTCTGGATGAAGTTA
  TTATGGGCTATAATTGCACTATCTTTGCGTATGGCCAAACTGGCACTGGAAAAACTTTTACAATGGAAGG
  TGAAAGGTCACCTAATGAAGAGTATACCTGGGAAGAGGATCCCTTGGCTGGTATAATTCCACGTACCCTT
  CATCAAATTTTTGAGAAACTTACTGATAATGGTACTGAATTTTCAGTCAAAGTGTCTCTGTTGGAGATCT
  ATAATGAAGAGCTTTTTGATCTTCTTAATCCATCATCTGATGTTTCTGAGAGACTACAGATGTTTGATGA
  TCCCCGTAACAAGAGAGGAGTGATAATTAAAGGTTTAGAAGAAATTACAGTACACAACAAGGATGAAGTC
  TATCAAATTTTAGAAAAGGGGGCAGCAAAAAGGACAACTGCAGCTACTCTGATGAATGCATACTCTAGTC
  GTTCCCACTCAGTTTTCTCTGTTACAATACATATGAAAGAAACTACGATTGATGGAGAAGAGCTTGTTAA
  AATCGGAAAGTTGAACTTGGTTGATCTTGCAGGAAGTGAAAACATTGGCCGTTCTGGAGCTGTTGATAAG
  AGAGCTCGGGAAGCTGGAAATATAAATCAATCCCTGTTGACTTTGGGAAGGGTCATTACTGCCCTTGTAG
  AAAGAACACCTCATGTTCCTTATCGAGAATCTAAACTAACTAGAATCCTCCAGGATTCTCTTGGAGGGCG
  TACAAGAACATCTATAATTGCAACAATTTCTCCTGCATCTCTCAATCTTGAGGAAACTCTGAGTACATTG
  GAATATGCTCATAGAGCAAAGAACATATTGAATAAGCCTGAAGTGAATCAGAAACTCACCAAAAAAGCTC
  TTATTAAGGAGTATACGGAGGAGATAGAACGTTTAAAACGAGATCTTGCTGCAGCCCGTGAGAAAAATGG
  AGTGTATATTTCTGAAGAAAATTTTAGAGTCATGAGTGGAAAATTAACTGTTCAAGAAGAGCAGATTGTA
  GAATTGATTGAAAAAATTGGTGCTGTTGAGGAGGAGCTGAATAGGGTTACAGAGTTGTTTATGGATAATA
  AAAATGAACTTGACCAGTGTAAATCTGACCTGCAAAATAAAACACAAGAACTTGAAACCACTCAAAAACA
  TTTGCAAGAAACTAAATTACAACTTGTTAAAGAAGAATATATCACATCAGCTTTGGAAAGTACTGAGGAG
  AAACTTCATGATGCTGCCAGCAAGCTGCTTAACACAGTTGAAGAAACTACAAAAGATGTATCTGGTCTCC
  ATTCCAAACTGGATCGTAAGAAGGCAGTTGACCAACACAATGCAGAAGCTCAGGATATTTTTGGCAAAAA
  CCTGAATAGTCTGTTTAATAATATGGAAGAATTAATTAAGGATGGCAGCTCAAAGCAAAAGGCCATGCTA
  GAAGTACATAAGACCTTATTTGGTAATCTGCTGTCTTCCAGTGTCTCTGCATTAGATACCATTACTACAG
  TAGCACTTGGATCTCTCACATCTATTCCAGAAAATGTGTCTACTCATGTTTCTCAGATTTTTAATATGAT
  ACTAAAAGAACAATCATTAGCAGCAGAAAGTAAAACTGTACTACAGGAATTGATTAATGTACTCAAGACT
  GATCTTCTAAGTTCACTGGAAATGATTTTATCCCCAACTGTGGTGTCTATACTGAAAATCAATAGTCAAC
  TAAAGCATATTTTCAAGACTTCATTGACAGTGGCCGATAAGATAGAAGATCAAAAAAAGGAACTAGATGG
  CTTTCTCAGTATACTGTGTAACAATCTACATGAACTACAAGAAAATACCATTTGTTCCTTGGTTGAGTCA
  CAAAAGCAATGTGGAAACCTAACTGAAGACCTGAAGACAATAAAGCAGACCCATTCCCAGGAACTTTGCA
  AGTTAATGAATCTTTGGACAGAGAGATTCTGTGCTTTGGAGGAAAAGTGTGAAAATATACAGAAACCACT
  TAGTAGTGTCCAGGAAAATATACAGCAGAAATCTAAGGATATAGTCAACAAAATGACTTTTCACAGTCAA
  AAATTTTGTGCTGATTCTGATGGCTTCTCACAGGAACTCAGAAATTTTAACCAAGAAGGTACAAAATTGG
  TTGAAGAATCTGTGAAACACTCTGATAAACTCAATGGCAACCTGGAAAAAATATCTCAAGAGACTGAACA
  GAGATGTGAATCTCTGAACACAAGAACAGTTTATTTTTCTGAACAGTGGGTATCTTCCTTAAATGAAAGG
  GAACAGGAACTTCACAACTTATTGGAGGTTGTAAGCCAATGTTGTGAGGCTTCAAGTTCAGACATCACTG
  AGAAATCAGATGGACGTAAGGCAGCTCATGAGAAACAGCATAACATTTTTCTTGATCAGATGACTATTGA
  TGAAGATAAATTGATAGCACAAAATCTAGAACTTAATGAAACCATAAAAATTGGTTTGACTAAGCTTAAT
  TGCTTTCTGGAACAGGATCTGAAACTGGATATCCCAACAGGTACGACACCACAGAGGAAAAGTTATTTAT
  ACCCATCAACACTGGTAAGAACTGAACCACGTGAACATCTCCTTGATCAGCTGAAAAGGAAACAGCCTGA
  GCTGTTAATGATGCTAAACTGTTCAGAAAACAACAAAGAAGAGACAATTCCGGATGTGGATGTAGAAGAG
  GCAGTTCTGGGGCAGTATACTGAAGAACCTCTAAGTCAAGAGCCATCTGTAGATGCTGGTGTGGATTGTT
  CATCAATTGGCGGGGTTCCATTTTTCCAGCATAAAAAATCACATGGAAAAGACAAAGAAAACAGAGGCAT
  TAACACACTGGAGAGGTCTAAAGTGGAAGAAACTACAGAGCACTTGGTTACAAAGAGCAGATTACCTCTG
  CGAGCCCAGATCAACCTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC218842 representing NM_004523
  Red=Cloning site Green=Tags(s)
  
  MASQPNSSAKKKEEKGKNIQVVVRCRPFNLAERKASAHSIVECDPVRKEVSVRTGGLADKSSRKTYTFDM
  VFGASTKQIDVYRSVVCPILDEVIMGYNCTIFAYGQTGTGKTFTMEGERSPNEEYTWEEDPLAGIIPRTL
  HQIFEKLTDNGTEFSVKVSLLEIYNEELFDLLNPSSDVSERLQMFDDPRNKRGVIIKGLEEITVHNKDEV
  YQILEKGAAKRTTAATLMNAYSSRSHSVFSVTIHMKETTIDGEELVKIGKLNLVDLAGSENIGRSGAVDK
  RAREAGNINQSLLTLGRVITALVERTPHVPYRESKLTRILQDSLGGRTRTSIIATISPASLNLEETLSTL
  EYAHRAKNILNKPEVNQKLTKKALIKEYTEEIERLKRDLAAAREKNGVYISEENFRVMSGKLTVQEEQIV
  ELIEKIGAVEEELNRVTELFMDNKNELDQCKSDLQNKTQELETTQKHLQETKLQLVKEEYITSALESTEE
  KLHDAASKLLNTVEETTKDVSGLHSKLDRKKAVDQHNAEAQDIFGKNLNSLFNNMEELIKDGSSKQKAML
  EVHKTLFGNLLSSSVSALDTITTVALGSLTSIPENVSTHVSQIFNMILKEQSLAAESKTVLQELINVLKT
  DLLSSLEMILSPTVVSILKINSQLKHIFKTSLTVADKIEDQKKELDGFLSILCNNLHELQENTICSLVES
  QKQCGNLTEDLKTIKQTHSQELCKLMNLWTERFCALEEKCENIQKPLSSVQENIQQKSKDIVNKMTFHSQ
  KFCADSDGFSQELRNFNQEGTKLVEESVKHSDKLNGNLEKISQETEQRCESLNTRTVYFSEQWVSSLNER
  EQELHNLLEVVSQCCEASSSDITEKSDGRKAAHEKQHNIFLDQMTIDEDKLIAQNLELNETIKIGLTKLN
  CFLEQDLKLDIPTGTTPQRKSYLYPSTLVRTEPREHLLDQLKRKQPELLMMLNCSENNKEETIPDVDVEE
  AVLGQYTEEPLSQEPSVDAGVDCSSIGGVPFFQHKKSHGKDKENRGINTLERSKVEETTEHLVTKSRLPL
  RAQINL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004523
• ORF Size: 3168 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004523.4
• RefSeq Size: 4908 bp
• RefSeq ORF: 3171 bp
• Locus ID: 3832
• UniProt ID: P52732
• Cytogenetics: 10q23.33
• Domains: kinesin
• Protein Families: Druggable Genome
• MW: 119 kDa
• Gene Summary: This gene encodes a motor protein that belongs to the kinesin-like protein family. Members of this protein family are known to be involved in various kinds of spindle dynamics. The function of this gene product includes chromosome positioning, centrosome separation and establishing a bipolar spindle during cell mitosis. [provided by RefSeq, Jul 2008]