Kinesin 5C (KIF5C) (NM_004522) Human Tagged ORF Clone

CAT#: RC218796

KIF5C (Myc-DDK-tagged)-Human kinesin family member 5C (KIF5C)

Product Images

Western blot validation of overexpression lysate (Cat# LY417922) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC218796 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified KIF5C protein (Cat# TP318796). The protein was produced from HEK293T cells transfected with KIF5C cDNA clone (Cat# RC218796) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: KIF5C
• Synonyms: CDCBM2; KINN; NKHC; NKHC-2; NKHC2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC218796 representing NM_004522
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGATCCAGCCGAATGCAGCATCAAAGTGATGTGCCGGTTCCGGCCCCTCAACGAAGCGGAGATCC
  TCCGCGGGGACAAATTCATCCCCAAATTTAAAGGCGATGAGACCGTGGTGATCGGGCAAGGGAAGCCATA
  TGTCTTCGACAGAGTGCTACCTCCCAACACGACCCAAGAGCAGGTTTACAATGCATGTGCGAAGCAAATT
  GTCAAAGATGTCCTTGAAGGTTATAACGGGACGATTTTTGCGTATGGGCAGACTTCATCAGGAAAAACCC
  ACACCATGGAGGGGAAGCTGCATGACCCCCAGCTCATGGGGATCATCCCACGAATTGCCCATGATATCTT
  TGACCATATCTACTCCATGGATGAGAACCTGGAGTTTCACATAAAGGTTTCCTATTTTGAGATCTACTTG
  GACAAAATAAGGGACTTACTTGATGTATCCAAGACCAACTTGGCTGTTCATGAAGATAAAAACAGAGTCC
  CGTATGTAAAGGGGTGCACTGAGCGGTTTGTGTCGAGCCCTGAGGAAGTCATGGATGTAATAGATGAAGG
  CAAAGCAAACCGACACGTGGCTGTGACAAACATGAATGAACACAGCTCTAGAAGTCACAGTATCTTCCTG
  ATAAATATTAAACAAGAGAATGTAGAGACTGAAAAAAAACTCAGTGGGAAACTTTATTTGGTTGATTTGG
  CTGGGAGCGAAAAGGTCAGCAAAACTGGTGCCGAGGGAGCTGTTCTTGACGAAGCTAAAAATATCAATAA
  GTCTTTGTCTGCTCTTGGAAATGTGATCTCTGCTTTGGCAGAAGGGACAAAAACACATGTGCCATACCGG
  GACAGCAAGATGACTCGGATTCTTCAGGACTCTTTGGGTGGGAACTGCAGAACCACCATCGTCATTTGCT
  GTTCTCCTTCTGTCTTCAATGAGGCTGAGACCAAGTCCACACTGATGTTCGGACAGAGAGCTAAGACCAT
  CAAGAATACAGTCTCTGTGAACCTAGAACTGACAGCAGAAGAATGGAAGAAGAAATATGAAAAAGAGAAA
  GAGAAAAACAAGACTTTGAAGAATGTTATCCAGCATCTGGAGATGGAGCTAAACAGGTGGAGGAATGGAG
  AAGCTGTGCCTGAGGATGAACAGATCAGTGCCAAGGACCAGAAGAACCTGGAGCCTTGTGATAACACCCC
  CATCATAGACAATATTGCTCCTGTTGTTGCTGGCATCTCTACAGAGGAGAAAGAGAAGTACGATGAGGAG
  ATCTCCAGTCTCTACAGACAACTGGATGACAAGGATGATGAAATTAACCAGCAGAGCCAGCTGGCTGAAA
  AGCTGAAGCAACAGATGTTGGATCAGGATGAGCTTTTAGCTTCCACAAGAAGAGACTATGAGAAGATACA
  GGAGGAGCTGACACGTCTCCAGATTGAAAATGAGGCAGCCAAGGATGAGGTGAAAGAAGTTCTCCAGGCC
  CTGGAGGAGCTGGCTGTCAATTATGACCAGAAATCACAGGAAGTGGAGGATAAGACCCGGGCCAATGAGC
  AGCTGACAGACGAGCTGGCCCAGAAAACGACTACATTGACAACCACACAGAGAGAGCTGAGCCAGCTACA
  AGAGCTTAGCAACCACCAGAAGAAAAGGGCAACTGAGATCCTGAATTTGCTGTTGAAAGATCTGGGGGAG
  ATAGGTGGAATTATTGGCACCAATGATGTGAAAACTTTGGCAGATGTGAATGGAGTCATTGAGGAGGAGT
  TTACCATGGCCCGCCTGTACATCAGCAAGATGAAGTCAGAGGTCAAGTCCCTGGTGAACCGCAGCAAACA
  GCTCGAGAGCGCCCAGATGGACTCCAACAGGAAGATGAATGCCAGCGAGCGGGAGCTGGCAGCCTGCCAG
  CTGCTCATCTCCCAGCACGAAGCCAAGATCAAGTCTCTGACAGACTACATGCAGAACATGGAACAGAAGA
  GGAGGCAGCTAGAAGAGTCCCAGGACTCGCTCAGCGAAGAGCTGGCAAAGCTCCGAGCCCAGGAAAAAAT
  GCACGAAGTCAGCTTCCAGGATAAGGAGAAGGAACATCTGACGCGGTTGCAGGATGCTGAAGAAATGAAG
  AAGGCGCTGGAGCAGCAGATGGAGAGCCACCGGGAAGCTCACCAGAAGCAGCTGTCCAGACTCCGAGACG
  AAATTGAGGAGAAGCAGAAAATCATTGATGAGATTCGGGATTTGAATCAGAAACTGCAACTGGAACAGGA
  GAAGCTTAGTTCTGATTATAACAAGCTGAAAATAGAGGACCAAGAGAGAGAAATGAAGCTGGAAAAGCTC
  TTATTGCTCAACGATAAAAGGGAACAAGCCAGAGAAGACCTCAAAGGGCTGGAGGAGACAGTGTCTAGAG
  AATTGCAGACACTGCACAACCTTCGGAAACTCTTTGTCCAGGATCTGACCACCCGAGTTAAAAAAAGTGT
  GGAGTTGGACAACGATGATGGAGGGGGCAGTGCTGCCCAGAAGCAGAAAATTTCCTTCTTGGAGAATAAC
  CTGGAGCAGCTCACCAAAGTTCACAAGCAGCTGGTCCGGGACAACGCAGACCTGCGCTGTGAACTGCCCA
  AGCTGGAGAAGCGGCTGCGTGCCACGGCGGAGCGCGTCAAGGCTCTGGAGAGCGCGCTGAAGGAGGCCAA
  GGAGAACGCCATGCGGGACCGTAAGCGCTACCAGCAGGAGGTGGATCGTATCAAGGAGGCCGTGCGGGCC
  AAGAACATGGCCAGAAGGGCCCATTCAGCCCAGATCGCCAAGCCCATCCGCCCCGGACACTACCCGGCCT
  CATCTCCAACGGCCGTCCATGCCATTCGAGGGGGAGGAGGCAGCTCTTCAAATTCCACTCACTACCAGAA
  A
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC218796 representing NM_004522
  Red=Cloning site Green=Tags(s)
  
  MADPAECSIKVMCRFRPLNEAEILRGDKFIPKFKGDETVVIGQGKPYVFDRVLPPNTTQEQVYNACAKQI
  VKDVLEGYNGTIFAYGQTSSGKTHTMEGKLHDPQLMGIIPRIAHDIFDHIYSMDENLEFHIKVSYFEIYL
  DKIRDLLDVSKTNLAVHEDKNRVPYVKGCTERFVSSPEEVMDVIDEGKANRHVAVTNMNEHSSRSHSIFL
  INIKQENVETEKKLSGKLYLVDLAGSEKVSKTGAEGAVLDEAKNINKSLSALGNVISALAEGTKTHVPYR
  DSKMTRILQDSLGGNCRTTIVICCSPSVFNEAETKSTLMFGQRAKTIKNTVSVNLELTAEEWKKKYEKEK
  EKNKTLKNVIQHLEMELNRWRNGEAVPEDEQISAKDQKNLEPCDNTPIIDNIAPVVAGISTEEKEKYDEE
  ISSLYRQLDDKDDEINQQSQLAEKLKQQMLDQDELLASTRRDYEKIQEELTRLQIENEAAKDEVKEVLQA
  LEELAVNYDQKSQEVEDKTRANEQLTDELAQKTTTLTTTQRELSQLQELSNHQKKRATEILNLLLKDLGE
  IGGIIGTNDVKTLADVNGVIEEEFTMARLYISKMKSEVKSLVNRSKQLESAQMDSNRKMNASERELAACQ
  LLISQHEAKIKSLTDYMQNMEQKRRQLEESQDSLSEELAKLRAQEKMHEVSFQDKEKEHLTRLQDAEEMK
  KALEQQMESHREAHQKQLSRLRDEIEEKQKIIDEIRDLNQKLQLEQEKLSSDYNKLKIEDQEREMKLEKL
  LLLNDKREQAREDLKGLEETVSRELQTLHNLRKLFVQDLTTRVKKSVELDNDDGGGSAAQKQKISFLENN
  LEQLTKVHKQLVRDNADLRCELPKLEKRLRATAERVKALESALKEAKENAMRDRKRYQQEVDRIKEAVRA
  KNMARRAHSAQIAKPIRPGHYPASSPTAVHAIRGGGGSSSNSTHYQK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004522
• ORF Size: 2871 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004522.3
• RefSeq Size: 6927 bp
• RefSeq ORF: 2874 bp
• Locus ID: 3800
• UniProt ID: O60282
• Cytogenetics: 2q23.1-q23.2
• Domains: kinesin
• Protein Families: Druggable Genome
• MW: 109.3 kDa
• Gene Summary: The protein encoded by this gene is a kinesin heavy chain subunit involved in the transport of cargo within the central nervous system. The encoded protein, which acts as a tetramer by associating with another heavy chain and two light chains, interacts with protein kinase CK2. Mutations in this gene have been associated with complex cortical dysplasia with other brain malformations-2. Two transcript variants, one protein-coding and the other non-protein coding, have been found for this gene. [provided by RefSeq, Jul 2015]