NLRP8 (NM_176811) Human Tagged ORF Clone

CAT#: RC218775

NLRP8 (Myc-DDK-tagged)-Human NLR family, pyrin domain containing 8 (NLRP8)

Product Images

Circular map for RC218775

• 

  Western blot validation of overexpression lysate (Cat# LY406131) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC218775 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified NLRP8 protein (Cat# TP318775). The protein was produced from HEK293T cells transfected with NLRP8 cDNA clone (Cat# RC218775) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NLRP8
• Synonyms: CLR19.2; NALP8; NOD16; PAN4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC218775 representing NM_176811
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTGACGTGAATCCACCCTCTGACACCCCCATTCCCTTTTCATCCTCCTCCACTCACAGTTCTCATA
  TTCCGCCCTGGACATTCTCTTGCTACCCCGGCTCCCCATGTGAAAATGGGGTCATGCTGTACATGAGAAA
  CGTGAGCCATGAGGAGCTACAACGGTTCAAGCAGCTCTTACTGACTGAGCTCAGTACTGGCACCATGCCC
  ATCACCTGGGACCAGGTCGAGACAGCCAGCTGGGCAGAGGTGGTTCATCTCTTGATAGAGCGTTTCCCTG
  GACGACGCGCTTGGGATGTGACTTCGAACATCTTTGCCATTATGAACTGTGATAAAATGTGTGTTGTAGT
  CCGCAGAGAGATAAATGCCATTCTGCCTACCTTGGAACCAGAGGACTTGAATGTGGGAGAAACACAGGTG
  AATCTGGAGGAAGGAGAATCTGGTAAAATACGGCGGTATAAATCGAATGTGATGGAAAAGTTTTTCCCCA
  TATGGGACATTACGACTTGGCCTGGAAACCAGAGGGACTTCTTCTACCAAGGTGTACACAGGCACGAGGA
  GTACTTACCATGTCTGCTTCTGCCCAAAAGACCCCAGGGTAGACAGCCCAAGACCGTGGCCATACAGGGA
  GCTCCTGGGATCGGAAAAACAATCCTGGCCAAAAAGGTGATGTTTGAGTGGGCCAGAAACAAGTTCTACG
  CCCACAAGCGCTGGTGTGCTTTCTACTTCCATTGCCAAGAGGTGAACCAGACGACAGACCAGAGCTTCTC
  CGAGCTGATTGAGCAAAAGTGGCCTGGATCTCAGGACCTCGTGTCAAAGATTATGTCCAAACCCGACCAA
  CTTCTGCTGCTCTTGGATGGCTTTGAGGAGCTCACATCTACCCTCATTGACAGACTGGAGGACCTGAGTG
  AAGACTGGAGGCAGAAATTGCCTGGGTCTGTCCTACTGAGCAGTTTGCTGAGCAAAACGATGCTTCCAGA
  GGCCACGCTACTGATCATGATAAGATTTACCTCTTGGCAGACATGCAAGCCCTTGCTGAAATGTCCCTCT
  CTCGTAACCCTTCCGGGGTTTAATACGATGGAAAAAATCAAGTATTTCCAGATGTATTTTGGACACACAG
  AGGAGGGAGACCAAGTCTTGAGTTTCGCCATGGAAAACACCATTCTCTTCTCCATGTGCCGGGTCCCTGT
  GGTTTGCTGGATGGTCTGCTCTGGTCTGAAACAGCAAATGGAGAGAGGAAACAATCTCACACAGTCATGT
  CCAAATGCCACCTCTGTGTTCGTCCGGTATATTTCTAGCTTGTTTCCCACCAGAGCTGAGAACTTTTCCA
  GAAAGATCCACCAAGCACAACTGGAAGGTCTGTGTCACTTGGCCGCAGACAGCATGTGGCACAGGAAATG
  GGTGTTAGGTAAAGAAGATCTTGAGGAAGCCAAGCTGGATCAGACGGGAGTCACCGCCTTCCTTGGCATG
  AGTATTCTTCGGAGAATTGCAGGTGAGGAAGACCACTATGTCTTTACCCTCGTGACTTTTCAGGAATTTT
  TTGCGGCCTTGTTTTATGTTCTCTGTTTCCCACAAAGACTCAAAAATTTTCATGTGTTGAGCCACGTGAA
  TATCCAGCGCCTGATAGCGAGTCCCAGAGGAAGCAAAAGCTATCTCTCTCACATGGGACTTTTCTTATTC
  GGTTTTCTGAACGAGGCCTGCGCTTCGGCCGTGGAACAGTCATTCCAATGCAAGGTGTCTTTCGGTAATA
  AGAGGAAACTGCTGAAAGTCATACCTCTGTTGCATAAATGTGACCCACCTTCTCCGGGCAGTGGGGTCCC
  GCAGTTATTCTACTGTCTGCATGAAATCCGGGAGGAAGCCTTTGTAAGCCAAGCCCTAAATGATTATCAT
  AAAGTTGTCTTGAGAATTGGCAACAACAAAGAAGTTCAAGTGTCTGCTTTTTGCCTGAAGCGGTGTCAAT
  ATTTGCATGAGGTGGAACTGACCGTCACCCTGAACTTCATGAACGTGTGGAAGCTCAGCTCCAGCTCCCA
  TCCTGGCTCTGAAGCGCCAGAGAGCAATGGGCTGCATCGTTGGTGGCAAGACTTATGCTCTGTGTTTGCA
  ACGAATGATAAGCTGGAAGTCCTGACTATGACCAACAGTGTTTTGGGGCCTCCTTTTTTGAAGGCTCTCG
  CGGCCGCACTGAGGCACCCTCAGTGCAAACTGCAAAAGCTACTCCTAAGGCGTGTGAATAGCACCATGTT
  GAACCAGGACTTAATCGGTGTTTTGACGGGGAACCAGCATCTGAGATACTTGGAAATACAACATGTGGAA
  GTGGAGTCCAAAGCTGTGAAGCTTCTATGCAGGGTGCTGAGATCCCCCCGGTGCCGTCTGCAGTGTCTCA
  GGTTGGAAGACTGCTTGGCCACCCCTAGAATTTGGACTGATCTTGGCAATAATCTTCAAGGTAACGGGCA
  TCTAAAGACTCTCATACTAAGAAAAAACTCCCTGGAGAACTGTGGGGCGTATTACCTGTCTGTGGCCCAG
  CTGGAGAGGCTGTCGATAGAGAACTGCAACCTTACACAGCTTACTTGTGAAAGCCTTGCCTCCTGTCTCA
  GGCAGAGTAAGATGCTGACCCACCTGAGCTTGGCAGAAAACGCCTTGAAAGATGAAGGGGCCAAGCATAT
  TTGGAATGCCCTGCCACACCTGAGATGTCCTCTGCAGAGGCTGGTACTGAGAAAGTGTGACTTGACCTTT
  AATTGCTGTCAGGATATGATCTCTGCGCTCTGTAAAAATAAAACCCTGAAAAGTCTTGACCTAAGTTTTA
  ATAGCCTGAAGGATGATGGGGTGATCCTGCTGTGTGAGGCCCTGAAGAACCCTGACTGTACATTACAGAT
  CCTGGAGCTGGAAAACTGCCTGTTCACCTCCATCTGCTGCCAGGCCATGGCTTCCATGCTCCGCAAAAAC
  CAACATCTGAGACATCTGGACTTGAGCAAGAATGCGATTGGAGTCTATGGTATTCTGACCTTGTGCGAGG
  CCTTCTCAAGCCAAAAGAAGAGAGAAGAGGTCATTTTCTGTATTCCTGCCTGGACTCGAATAACTAGCTT
  CTCCCCAACTCCTCACCCACCCGACTTCACGGGAAAAAGTGACTGCCTATCCCAGATTAATCCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC218775 representing NM_176811
  Red=Cloning site Green=Tags(s)
  
  MSDVNPPSDTPIPFSSSSTHSSHIPPWTFSCYPGSPCENGVMLYMRNVSHEELQRFKQLLLTELSTGTMP
  ITWDQVETASWAEVVHLLIERFPGRRAWDVTSNIFAIMNCDKMCVVVRREINAILPTLEPEDLNVGETQV
  NLEEGESGKIRRYKSNVMEKFFPIWDITTWPGNQRDFFYQGVHRHEEYLPCLLLPKRPQGRQPKTVAIQG
  APGIGKTILAKKVMFEWARNKFYAHKRWCAFYFHCQEVNQTTDQSFSELIEQKWPGSQDLVSKIMSKPDQ
  LLLLLDGFEELTSTLIDRLEDLSEDWRQKLPGSVLLSSLLSKTMLPEATLLIMIRFTSWQTCKPLLKCPS
  LVTLPGFNTMEKIKYFQMYFGHTEEGDQVLSFAMENTILFSMCRVPVVCWMVCSGLKQQMERGNNLTQSC
  PNATSVFVRYISSLFPTRAENFSRKIHQAQLEGLCHLAADSMWHRKWVLGKEDLEEAKLDQTGVTAFLGM
  SILRRIAGEEDHYVFTLVTFQEFFAALFYVLCFPQRLKNFHVLSHVNIQRLIASPRGSKSYLSHMGLFLF
  GFLNEACASAVEQSFQCKVSFGNKRKLLKVIPLLHKCDPPSPGSGVPQLFYCLHEIREEAFVSQALNDYH
  KVVLRIGNNKEVQVSAFCLKRCQYLHEVELTVTLNFMNVWKLSSSSHPGSEAPESNGLHRWWQDLCSVFA
  TNDKLEVLTMTNSVLGPPFLKALAAALRHPQCKLQKLLLRRVNSTMLNQDLIGVLTGNQHLRYLEIQHVE
  VESKAVKLLCRVLRSPRCRLQCLRLEDCLATPRIWTDLGNNLQGNGHLKTLILRKNSLENCGAYYLSVAQ
  LERLSIENCNLTQLTCESLASCLRQSKMLTHLSLAENALKDEGAKHIWNALPHLRCPLQRLVLRKCDLTF
  NCCQDMISALCKNKTLKSLDLSFNSLKDDGVILLCEALKNPDCTLQILELENCLFTSICCQAMASMLRKN
  QHLRHLDLSKNAIGVYGILTLCEAFSSQKKREEVIFCIPAWTRITSFSPTPHPPDFTGKSDCLSQINP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_176811
• ORF Size: 3144 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_176811.2, NP_789781.2
• RefSeq Size: 3934 bp
• RefSeq ORF: 3147 bp
• Locus ID: 126205
• UniProt ID: Q86W28
• Cytogenetics: 19q13.43
• MW: 119.2 kDa
• Gene Summary: This gene encodes a member of the nucleotide-binding oligomerization domain/ leucine rich repeat/ pyrin domain containing (NLRP) subfamily, which belongs to the Nod-like receptor family of proteins. NLRP genes play roles in the mammalian innate immune system through inflammasome formation and activation of caspases. In addition, NLRP genes have been found to function during mammalian reproduction. Consistent with a function during human preimplantation development, this gene is expressed at high levels in oocytes with decreased levels in embryos. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2016]