P2X3 (P2RX3) (NM_002559) Human Tagged ORF Clone

CAT#: RC218626

P2RX3 (Myc-DDK-tagged)-Human purinergic receptor P2X, ligand-gated ion channel, 3 (P2RX3)

Product Images

Circular map for RC218626

• 

  Western blot validation of overexpression lysate (Cat# LY419263) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC218626 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified P2RX3 protein (Cat# TP318626). The protein was produced from HEK293T cells transfected with P2RX3 cDNA clone (Cat# RC218626) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: P2X3
• Synonyms: P2X3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC218626 representing NM_002559
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACTGCATATCCGACTTCTTCACCTATGAGACCACCAAGTCGGTGGTTGTGAAGAGCTGGACCATCG
  GGATCATCAACCGAGTAGTTCAGCTTCTGATCATCTCCTACTTTGTAGGGTGGGTTTTCTTGCACGAGAA
  GGCTTACCAGGTACGGGACACAGCCATTGAGTCCTCGGTGGTAACCAAGGTGAAGGGCTCCGGACTCTAC
  GCCAACAGAGTCATGGATGTGTCTGATTACGTGACGCCACCTCAGGGCACCTCGGTCTTTGTCATCATCA
  CCAAGATGATTGTTACTGAAAATCAGATGCAAGGATTCTGCCCAGAGAGTGAGGAGAAATACCGCTGTGT
  ATCAGACAGCCAGTGCGGGCCTGAGCGCTTGCCAGGTGGGGGGATCCTCACTGGCCGCTGCGTGAACTAC
  AGCTCTGTGCTCCGGACCTGTGAGATCCAGGGCTGGTGCCCCACGGAGGTGGACACAGTGGAAACGCCCA
  TCATGATGGAAGCTGAGAACTTCACTATTTTCATCAAGAACAGCATCCGTTTCCCCCTCTTCAACTTTGA
  GAAGGGAAACCTCCTTCCCAACCTGACAGCCAGGGACATGAAGACCTGCCGCTTCCACCCGGACAAGGAC
  CCTTTCTGCCCCATCTTGCGGGTAGGGGACGTGGTCAAGTTTGCGGGGCAGGATTTTGCCAAACTGGCGC
  GCACGGGGGGAGTTCTGGGCATTAAGATCGGCTGGGTGTGCGACTTGGACAAGGCCTGGGACCAGTGCAT
  CCCCAAATACTCCTTCACCCGGCTCGACAGCGTTTCTGAGAAAAGCAGCGTGTCCCCAGGCTACAACTTC
  AGGTTTGCCAAGTACTACAAAATGGAAAATGGCAGTGAGTACCGCACCCTCCTGAAGGCTTTTGGCATCC
  GCTTCGACGTGCTGGTATACGGGAATGCTGGCAAGTTCAACATCATCCCCACCATCATCAGCTCTGTGGC
  GGCCTTTACTTCTGTGGGAGTGGGAACTGTTCTCTGTGACATCATCCTGCTCAACTTCCTCAAGGGGGCC
  GACCAGTACAAAGCCAAGAAGTTTGAGGAGGTGAATGAGACTACACTGAAAATCGCGGCTTTGACCAACC
  CAGTGTACCCCAGCGACCAGACCACAGTGGAGAAGCAGTCCACCGATTCGGGGGCCTTCTCCATAGGCCA
  C
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC218626 representing NM_002559
  Red=Cloning site Green=Tags(s)
  
  MNCISDFFTYETTKSVVVKSWTIGIINRVVQLLIISYFVGWVFLHEKAYQVRDTAIESSVVTKVKGSGLY
  ANRVMDVSDYVTPPQGTSVFVIITKMIVTENQMQGFCPESEEKYRCVSDSQCGPERLPGGGILTGRCVNY
  SSVLRTCEIQGWCPTEVDTVETPIMMEAENFTIFIKNSIRFPLFNFEKGNLLPNLTARDMKTCRFHPDKD
  PFCPILRVGDVVKFAGQDFAKLARTGGVLGIKIGWVCDLDKAWDQCIPKYSFTRLDSVSEKSSVSPGYNF
  RFAKYYKMENGSEYRTLLKAFGIRFDVLVYGNAGKFNIIPTIISSVAAFTSVGVGTVLCDIILLNFLKGA
  DQYKAKKFEEVNETTLKIAALTNPVYPSDQTTVEKQSTDSGAFSIGH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002559
• ORF Size: 1191 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002559.5
• RefSeq Size: 1349 bp
• RefSeq ORF: 1194 bp
• Locus ID: 5024
• UniProt ID: P56373
• Cytogenetics: 11q12.1
• Protein Families: Druggable Genome, Ion Channels: ATP Receptors, Transmembrane
• Protein Pathways: Calcium signaling pathway, Neuroactive ligand-receptor interaction
• MW: 44.1 kDa
• Gene Summary: This gene encodes a member of the P2X purinergic receptor (purinoceptor) gene family which includes seven members (P2RX1 - P2RX7). P2X purinoceptors are a family of cation-permeable, ligand-gated ion channels that open in response to the binding of extracellular adenosine 5'-triphosphate (ATP). The encoded protein is a subunit of the trimeric P2X3 receptor ion channel which is expressed by sensory or autonomic neurons. A deficiency of the orthologous protein in mice is associated with reduced pain-related behavior and urinary bladder hyporeflexia. [provided by RefSeq, Aug 2017]