TRIM47 (NM_033452) Human Tagged ORF Clone

CAT#: RC218521

TRIM47 (Myc-DDK-tagged)-Human tripartite motif containing 47 (TRIM47)

Product Images

Western blot validation of overexpression lysate (Cat# LY409515) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC218521 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified TRIM47 protein (Cat# TP318521). The protein was produced from HEK293T cells transfected with TRIM47 cDNA clone (Cat# RC218521) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TRIM47
• Synonyms: GOA; RNF100
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC218521 representing NM_033452
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACGGCAGTGGACCCTTCAGCTGCCCCATCTGCCTAGAGCCACTCCGGGAGCCGGTGACGCTGCCCT
  GCGGCCACAACTTCTGTCTCGCCTGCCTGGGCGCGCTCTGGCCGCATCGTGGCGCGAGTGGAGCCGGCGG
  ACCCGGAGGCGCGGCCCGCTGCCCGCTGTGCCAGGAGCCCTTCCCCGACGGCCTTCAGCTCCGCAAGAAC
  CACACGCTGTCCGAGCTGCTGCAGCTCCGCCAGGGCTCGGGCCCCGGGTCCGGCCCCGGCCCGGCCCCTG
  CCCTGGCCCCGGAGCCCTCGGCACCCAGCGCGCTGCCCAGTGTCCCGGAGCCGTCGGCCCCCTGCGCTCC
  CGAGCCGTGGCCCGCGGGCGAAGAGCCAGTGCGCTGCGACGCGTGCCCCGAGGGCGCGGCCCTGCCCGCC
  GCGCTGTCCTGCCTCTCCTGCCTCGCCTCCTTTTGCCCCGCGCACCTGGGCCCGCACGAGCGCAGCCCCG
  CCCTCCGCGGACACCGCCTGGTGCCGCCGCTGCGCCGGCTAGAGGAGAGCCTGTGCCCGCGCCACCTACG
  GCCGCTCGAGCGCTACTGCCGCGCGGAGCGCGTGTGTCTGTGCGAGGCCTGCGCCGCACAGGAGCACCGC
  GGCCACGAGCTGGTGCCGCTGGAGCAGGAGCGCGCGCTTCAGGAGGCTGAGCAGTCCAAAGTCCTGAGCG
  CCGTGGAGGACCGCATGGACGAGCTGGGTGCTGGCATTGCACAGTCCAGGCGCACAGTGGCCCTCATCAA
  GAGTGCAGCCGTAGCAGAGCGGGAGAGGGTGAGCCGGCTGTTTGCAGATGCTGCGGCCGCCCTGCAGGGC
  TTCCAGACCCAGGTGCTGGGCTTCATCGAGGAGGGGGAAGCTGCCATGCTAGGCCGCTCCCAGGGTGACC
  TGCGGCGACAGGAGGAACAGCGCAGCCGCCTGAGCCGAGCCCGCCAGAATCTCAGCCAGGTCCCTGAAGC
  TGACTCAGTCAGCTTCCTGCAGGAGCTGCTGGCACTAAGGCTGGCCCTGGAGGATGGGTGTGGCCCTGGG
  CCTGGACCCCCGAGGGAGCTCAGCTTCACCAAATCATCCCAAGCTGTCCGTGCAGTGAGAGACATGCTGG
  CCGTGGCCTGCGTCAACCAGTGGGAGCAGCTGAGGGGGCCGGGTGGCAACGAGGATGGGCCACAGAAGCT
  GGACTCGGAAGCTGATGCTGAGCCCCAAGACCTCGAGAGTACGAACCTCTTGGAGAGTGAAGCTCCCAGG
  GACTATTTCCTCAAGTTTGCCTATATTGTGGATTTGGACAGCGACACAGCAGACAAGTTCCTGCAGCTGT
  TTGGAACCAAAGGTGTCAAGAGGGTGCTGTGTCCTATCAACTACCCCTTGTCGCCCACCCGCTTCACCCA
  TTGTGAGCAGGTGCTGGGCGAGGGTGCCCTGGACCGAGGCACCTACTACTGGGAGGTGGAGATTATCGAG
  GGCTGGGTCAGCATGGGGGTCATGGCCGAAGACTTCTCCCCACAAGAGCCCTACGACCGCGGCCGGCTGG
  GCCGCAACGCCCACTCCTGCTGCCTGCAGTGGAATGGACGCAGCTTCTCCGTCTGGTTTCATGGGCTGGA
  GGCTCCCCTGCCCCACCCCTTCTCGCCCACGGTTGGGGTCTGCCTGGAATACGCTGACCGTGCCTTGGCC
  TTCTATGCTGTACGGGACGGCAAGATGAGCCTCCTGCGGAGGCTGAAGGCCTCCCGGCCCCGCCGGGGTG
  GCATCCCGGCCTCCCCCATTGACCCCTTCCAGAGCCGCCTGGACAGTCACTTTGCGGGGCTCTTCACCCA
  CAGACTCAAGCCTGCCTTCTTCCTGGAGAGTGTGGACGCCCACTTGCAGATCGGGCCCCTCAAGAAGTCC
  TGCATATCCGTGCTGAAGAGGAGG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC218521 representing NM_033452
  Red=Cloning site Green=Tags(s)
  
  MDGSGPFSCPICLEPLREPVTLPCGHNFCLACLGALWPHRGASGAGGPGGAARCPLCQEPFPDGLQLRKN
  HTLSELLQLRQGSGPGSGPGPAPALAPEPSAPSALPSVPEPSAPCAPEPWPAGEEPVRCDACPEGAALPA
  ALSCLSCLASFCPAHLGPHERSPALRGHRLVPPLRRLEESLCPRHLRPLERYCRAERVCLCEACAAQEHR
  GHELVPLEQERALQEAEQSKVLSAVEDRMDELGAGIAQSRRTVALIKSAAVAERERVSRLFADAAAALQG
  FQTQVLGFIEEGEAAMLGRSQGDLRRQEEQRSRLSRARQNLSQVPEADSVSFLQELLALRLALEDGCGPG
  PGPPRELSFTKSSQAVRAVRDMLAVACVNQWEQLRGPGGNEDGPQKLDSEADAEPQDLESTNLLESEAPR
  DYFLKFAYIVDLDSDTADKFLQLFGTKGVKRVLCPINYPLSPTRFTHCEQVLGEGALDRGTYYWEVEIIE
  GWVSMGVMAEDFSPQEPYDRGRLGRNAHSCCLQWNGRSFSVWFHGLEAPLPHPFSPTVGVCLEYADRALA
  FYAVRDGKMSLLRRLKASRPRRGGIPASPIDPFQSRLDSHFAGLFTHRLKPAFFLESVDAHLQIGPLKKS
  CISVLKRR
  
  SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_033452
• ORF Size: 1914 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_033452.3
• RefSeq Size: 2268 bp
• RefSeq ORF: 1917 bp
• Locus ID: 91107
• UniProt ID: Q96LD4
• Cytogenetics: 17q25.1
• Protein Families: Druggable Genome
• MW: 69.4 kDa
• Gene Summary: E3 ubiquitin-protein ligase that mediates the ubiquitination and proteasomal degradation of CYLD.[UniProtKB/Swiss-Prot Function]