MEK1 (MAP2K1) (NM_002755) Human Tagged ORF Clone
Lenti ORF clone of Human mitogen-activated protein kinase kinase 1 (MAP2K1), Myc-DDK-tagged
|Product Name||MEK1 (MAP2K1) (NM_002755) Human Tagged ORF Clone|
|Type||Human Tagged ORF Clone|
|Synonyms||CFC3; MAPKK1; MEK1; MKK1; PRKMK1|
|E. coli Selection||Chloramphenicol (34 ug/mL)|
|Restriction Sites||SgfI-MluI Cloning Scheme for this gene|
|ORF Size||1179 bp|
|OTI Disclaimer||Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at firstname.lastname@example.org or by calling 301.340.3188 option 3 for pricing and delivery.
The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
|OTI Annotation||This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.|
|Product Components||The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.|
|Reconstitution||1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
|RefSeq Size||2222 bp|
|RefSeq ORF||1182 bp|
|Domains||pkinase, TyrKc, S_TKc|
|Protein Families||Druggable Genome, Protein Kinase|
|Protein Pathways||Acute myeloid leukemia, B cell receptor signaling pathway, Bladder cancer, Chemokine signaling pathway, Chronic myeloid leukemia, Colorectal cancer, Dorso-ventral axis formation, Endometrial cancer, ErbB signaling pathway, Fc epsilon RI signaling pathway, Fc gamma R-mediated phagocytosis, Focal adhesion, Gap junction, Glioma, GnRH signaling pathway, Insulin signaling pathway, Long-term depression, Long-term potentiation, MAPK signaling pathway, Melanogenesis, Melanoma, Natural killer cell mediated cytotoxicity, Neurotrophin signaling pathway, Non-small cell lung cancer, Oocyte meiosis, Pancreatic cancer, Pathways in cancer, Prion diseases, Progesterone-mediated oocyte maturation, Prostate cancer, Regulation of actin cytoskeleton, Renal cell carcinoma, T cell receptor signaling pathway, Thyroid cancer, Toll-like receptor signaling pathway, Vascular smooth muscle contraction, VEGF signaling pathway|
|Gene Summary||The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development. [provided by RefSeq, Jul 2008]|
|cDNA Clone Resources|
|SC118424||MAP2K1 (untagged)-Human mitogen-activated protein kinase kinase 1 (MAP2K1)||
|SC323696||MAP2K1 (untagged)-Kinase deficient mutant (K97M) of Human mitogen-activated protein kinase kinase 1 (MAP2K1)||
|RC218460||MAP2K1 (Myc-DDK-tagged)-Human mitogen-activated protein kinase kinase 1 (MAP2K1)||
|RG218460||MAP2K1 (GFP-tagged) - Human mitogen-activated protein kinase kinase 1 (MAP2K1)||
|RC218460L1||Lenti ORF clone of Human mitogen-activated protein kinase kinase 1 (MAP2K1), Myc-DDK-tagged||
|RC218460L2||Lenti ORF clone of Human mitogen-activated protein kinase kinase 1 (MAP2K1), mGFP tagged||
|RC218460L4||Lenti ORF clone of Human mitogen-activated protein kinase kinase 1 (MAP2K1), mGFP tagged||