KLHL3 (NM_017415) Human Tagged ORF Clone

CAT#: RC218393

KLHL3 (Myc-DDK-tagged)-Human kelch-like 3 (Drosophila) (KLHL3)

Product Images

Western blot validation of overexpression lysate (Cat# LY413773) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC218393 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified KLHL3 protein (Cat# TP318393). The protein was produced from HEK293T cells transfected with KLHL3 cDNA clone (Cat# RC218393) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: KLHL3
• Synonyms: PHA2D
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC218393 representing NM_017415
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGGTGAAAGTGTCAAGCTGAGCTCCCAGACTCTGATACAGGCTGGGGATGATGAGAAGAACCAGA
  GGACGATCACTGTCAACCCTGCCCACATGGGGAAAGCATTCAAGGTTATGAATGAACTGCGGAGTAAACA
  GCTGTTGTGTGACGTGATGATTGTGGCAGAAGATGTCGAGATAGAAGCCCACCGTGTGGTCCTGGCAGCC
  TGCAGCCCCTACTTCTGTGCGATGTTCACAGGTGACATGTCTGAGAGTAAAGCCAAAAAGATAGAAATCA
  AGGACGTGGATGGGCAGACGCTGAGTAAGCTGATTGACTACATCTATACTGCTGAAATCGAGGTGACTGA
  AGAGAATGTCCAGGTGCTGCTCCCGGCAGCCAGCTTGCTGCAGCTCATGGATGTTCGGCAGAACTGCTGT
  GACTTCCTGCAGTCTCAGTTGCATCCCACCAATTGCCTGGGCATCCGTGCATTTGCAGATGTACACACCT
  GCACTGACCTTCTGCAGCAGGCCAATGCCTACGCAGAGCAGCACTTTCCAGAGGTGATGCTAGGAGAAGA
  ATTTCTTAGCCTGAGTCTGGACCAGGTGTGCAGCTTGATATCCAGCGACAAGCTGACCGTTTCTTCAGAA
  GAGAAGGTGTTTGAAGCTGTGATCTCATGGATCAATTATGAGAAAGAAACCCGTTTAGAGCACATGGCAA
  AGCTGATGGAACATGTCCGACTTCCTCTCTTACCTAGGGACTACCTAGTCCAAACGGTTGAAGAAGAAGC
  TTTGATAAAGAATAACAACACCTGTAAAGACTTCCTCATTGAGGCCATGAAATACCATCTCCTCCCTCTG
  GATCAGAGACTATTGATTAAGAACCCAAGGACCAAGCCCAGGACTCCAGTCAGCCTTCCCAAGGTCATGA
  TTGTGGTTGGCGGCCAGGCACCCAAGGCAATCCGCAGTGTGGAGTGCTATGATTTCGAGGAGGACCGGTG
  GGATCAGATTGCTGAGCTTCCTTCCAGAAGATGCAGAGCAGGTGTGGTGTTCATGGCTGGCCACGTGTAT
  GCCGTGGGAGGGTTTAATGGCTCACTGCGGGTGCGGACAGTGGATGTGTATGACGGCGTGAAGGACCAGT
  GGACGTCCATTGCCAGCATGCAGGAGCGCCGGAGCACACTGGGCGCAGCGGTGCTCAATGACTTGCTCTA
  CGCAGTGGGAGGCTTTGATGGCAGTACTGGCCTAGCATCGGTGGAAGCCTACAGCTACAAGACCAACGAG
  TGGTTCTTTGTGGCCCCGATGAACACGCGGCGGAGCAGTGTGGGTGTGGGCGTTGTGGAGGGGAAGCTAT
  ATGCTGTTGGGGGTTATGATGGAGCTTCCCGCCAGTGTCTGAGCACTGTGGAGCAGTACAACCCAGCGAC
  CAATGAATGGATATACGTGGCGGACATGAGCACCCGCCGCAGTGGCGCAGGGGTTGGAGTGCTTAGCGGA
  CAGCTGTACGCCACAGGTGGGCATGATGGGCCTTTGGTGAGGAAGAGCGTTGAGGTTTACGATCCTGGAA
  CAAATACCTGGAAGCAAGTGGCAGACATGAACATGTGCCGGCGCAACGCAGGGGTCTGTGCAGTAAATGG
  GCTCCTGTATGTGGTTGGAGGGGATGATGGATCCTGCAACTTGGCTTCGGTGGAGTACTACAATCCTGTC
  ACTGACAAATGGACGCTGCTTCCAACGAACATGAGCACGGGGCGGAGCTATGCAGGTGTTGCCGTGATTC
  ACAAGTCCTTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC218393 representing NM_017415
  Red=Cloning site Green=Tags(s)
  
  MEGESVKLSSQTLIQAGDDEKNQRTITVNPAHMGKAFKVMNELRSKQLLCDVMIVAEDVEIEAHRVVLAA
  CSPYFCAMFTGDMSESKAKKIEIKDVDGQTLSKLIDYIYTAEIEVTEENVQVLLPAASLLQLMDVRQNCC
  DFLQSQLHPTNCLGIRAFADVHTCTDLLQQANAYAEQHFPEVMLGEEFLSLSLDQVCSLISSDKLTVSSE
  EKVFEAVISWINYEKETRLEHMAKLMEHVRLPLLPRDYLVQTVEEEALIKNNNTCKDFLIEAMKYHLLPL
  DQRLLIKNPRTKPRTPVSLPKVMIVVGGQAPKAIRSVECYDFEEDRWDQIAELPSRRCRAGVVFMAGHVY
  AVGGFNGSLRVRTVDVYDGVKDQWTSIASMQERRSTLGAAVLNDLLYAVGGFDGSTGLASVEAYSYKTNE
  WFFVAPMNTRRSSVGVGVVEGKLYAVGGYDGASRQCLSTVEQYNPATNEWIYVADMSTRRSGAGVGVLSG
  QLYATGGHDGPLVRKSVEVYDPGTNTWKQVADMNMCRRNAGVCAVNGLLYVVGGDDGSCNLASVEYYNPV
  TDKWTLLPTNMSTGRSYAGVAVIHKSL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017415
• ORF Size: 1761 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017415.3
• RefSeq Size: 6485 bp
• RefSeq ORF: 1764 bp
• Locus ID: 26249
• UniProt ID: Q9UH77
• Cytogenetics: 5q31.2
• Domains: BTB, Kelch
• MW: 64.8 kDa
• Gene Summary: This gene is ubiquitously expressed and encodes a full-length protein which has an N-terminal BTB domain followed by a BACK domain and six kelch-like repeats in the C-terminus. These kelch-like repeats promote substrate ubiquitination of bound proteins via interaction of the BTB domain with the CUL3 (cullin 3) component of a cullin-RING E3 ubiquitin ligase (CRL) complex. Muatations in this gene cause pseudohypoaldosteronism type IID (PHA2D); a rare Mendelian syndrome featuring hypertension, hyperkalaemia and metabolic acidosis. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Mar 2012]