KLHL3 (NM_017415) Human Tagged ORF Clone

CAT#: RC218393

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KLHL3 (Myc-DDK-tagged)-Human kelch-like 3 (Drosophila) (KLHL3)



  "NM_017415" in other vectors (4)

Reconstitution Protocol

USD 546.00


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In Stock

Size
    • 10 ug


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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol KLHL3
Synonyms PHA2D
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC218393 representing NM_017415
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGGGTGAAAGTGTCAAGCTGAGCTCCCAGACTCTGATACAGGCTGGGGATGATGAGAAGAACCAGA
GGACGATCACTGTCAACCCTGCCCACATGGGGAAAGCATTCAAGGTTATGAATGAACTGCGGAGTAAACA
GCTGTTGTGTGACGTGATGATTGTGGCAGAAGATGTCGAGATAGAAGCCCACCGTGTGGTCCTGGCAGCC
TGCAGCCCCTACTTCTGTGCGATGTTCACAGGTGACATGTCTGAGAGTAAAGCCAAAAAGATAGAAATCA
AGGACGTGGATGGGCAGACGCTGAGTAAGCTGATTGACTACATCTATACTGCTGAAATCGAGGTGACTGA
AGAGAATGTCCAGGTGCTGCTCCCGGCAGCCAGCTTGCTGCAGCTCATGGATGTTCGGCAGAACTGCTGT
GACTTCCTGCAGTCTCAGTTGCATCCCACCAATTGCCTGGGCATCCGTGCATTTGCAGATGTACACACCT
GCACTGACCTTCTGCAGCAGGCCAATGCCTACGCAGAGCAGCACTTTCCAGAGGTGATGCTAGGAGAAGA
ATTTCTTAGCCTGAGTCTGGACCAGGTGTGCAGCTTGATATCCAGCGACAAGCTGACCGTTTCTTCAGAA
GAGAAGGTGTTTGAAGCTGTGATCTCATGGATCAATTATGAGAAAGAAACCCGTTTAGAGCACATGGCAA
AGCTGATGGAACATGTCCGACTTCCTCTCTTACCTAGGGACTACCTAGTCCAAACGGTTGAAGAAGAAGC
TTTGATAAAGAATAACAACACCTGTAAAGACTTCCTCATTGAGGCCATGAAATACCATCTCCTCCCTCTG
GATCAGAGACTATTGATTAAGAACCCAAGGACCAAGCCCAGGACTCCAGTCAGCCTTCCCAAGGTCATGA
TTGTGGTTGGCGGCCAGGCACCCAAGGCAATCCGCAGTGTGGAGTGCTATGATTTCGAGGAGGACCGGTG
GGATCAGATTGCTGAGCTTCCTTCCAGAAGATGCAGAGCAGGTGTGGTGTTCATGGCTGGCCACGTGTAT
GCCGTGGGAGGGTTTAATGGCTCACTGCGGGTGCGGACAGTGGATGTGTATGACGGCGTGAAGGACCAGT
GGACGTCCATTGCCAGCATGCAGGAGCGCCGGAGCACACTGGGCGCAGCGGTGCTCAATGACTTGCTCTA
CGCAGTGGGAGGCTTTGATGGCAGTACTGGCCTAGCATCGGTGGAAGCCTACAGCTACAAGACCAACGAG
TGGTTCTTTGTGGCCCCGATGAACACGCGGCGGAGCAGTGTGGGTGTGGGCGTTGTGGAGGGGAAGCTAT
ATGCTGTTGGGGGTTATGATGGAGCTTCCCGCCAGTGTCTGAGCACTGTGGAGCAGTACAACCCAGCGAC
CAATGAATGGATATACGTGGCGGACATGAGCACCCGCCGCAGTGGCGCAGGGGTTGGAGTGCTTAGCGGA
CAGCTGTACGCCACAGGTGGGCATGATGGGCCTTTGGTGAGGAAGAGCGTTGAGGTTTACGATCCTGGAA
CAAATACCTGGAAGCAAGTGGCAGACATGAACATGTGCCGGCGCAACGCAGGGGTCTGTGCAGTAAATGG
GCTCCTGTATGTGGTTGGAGGGGATGATGGATCCTGCAACTTGGCTTCGGTGGAGTACTACAATCCTGTC
ACTGACAAATGGACGCTGCTTCCAACGAACATGAGCACGGGGCGGAGCTATGCAGGTGTTGCCGTGATTC
ACAAGTCCTTG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC218393 representing NM_017415
Red=Cloning site Green=Tags(s)

MEGESVKLSSQTLIQAGDDEKNQRTITVNPAHMGKAFKVMNELRSKQLLCDVMIVAEDVEIEAHRVVLAA
CSPYFCAMFTGDMSESKAKKIEIKDVDGQTLSKLIDYIYTAEIEVTEENVQVLLPAASLLQLMDVRQNCC
DFLQSQLHPTNCLGIRAFADVHTCTDLLQQANAYAEQHFPEVMLGEEFLSLSLDQVCSLISSDKLTVSSE
EKVFEAVISWINYEKETRLEHMAKLMEHVRLPLLPRDYLVQTVEEEALIKNNNTCKDFLIEAMKYHLLPL
DQRLLIKNPRTKPRTPVSLPKVMIVVGGQAPKAIRSVECYDFEEDRWDQIAELPSRRCRAGVVFMAGHVY
AVGGFNGSLRVRTVDVYDGVKDQWTSIASMQERRSTLGAAVLNDLLYAVGGFDGSTGLASVEAYSYKTNE
WFFVAPMNTRRSSVGVGVVEGKLYAVGGYDGASRQCLSTVEQYNPATNEWIYVADMSTRRSGAGVGVLSG
QLYATGGHDGPLVRKSVEVYDPGTNTWKQVADMNMCRRNAGVCAVNGLLYVVGGDDGSCNLASVEYYNPV
TDKWTLLPTNMSTGRSYAGVAVIHKSL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_017415
ORF Size 1761 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_017415.3
RefSeq Size 6485 bp
RefSeq ORF 1764 bp
Locus ID 26249
UniProt ID Q9UH77
Cytogenetics 5q31.2
Domains BTB, Kelch
MW 64.8 kDa
Gene Summary This gene is ubiquitously expressed and encodes a full-length protein which has an N-terminal BTB domain followed by a BACK domain and six kelch-like repeats in the C-terminus. These kelch-like repeats promote substrate ubiquitination of bound proteins via interaction of the BTB domain with the CUL3 (cullin 3) component of a cullin-RING E3 ubiquitin ligase (CRL) complex. Muatations in this gene cause pseudohypoaldosteronism type IID (PHA2D); a rare Mendelian syndrome featuring hypertension, hyperkalaemia and metabolic acidosis. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Mar 2012]

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