LNK (SH2B3) (NM_005475) Human Tagged ORF Clone

CAT#: RC218359

SH2B3 (Myc-DDK-tagged)-Human SH2B adaptor protein 3 (SH2B3)

Product Images

Circular map for RC218359

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY SH2B3 (Cat# RC218359, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SH2B3(Cat# TA502933). Positive lysates LY401678 (100ug) and LC401678 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY401678) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC218359 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified SH2B3 protein (Cat# TP318359). The protein was produced from HEK293T cells transfected with SH2B3 cDNA clone (Cat# RC218359) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: LNK
• Synonyms: IDDM20; LNK
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC218359 representing NM_005475
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACGGGCCTGCCCTGCAGCCCTCCTCGCCCTCTTCCGCGCCCTCAGCCTCCCCGGCGGCGGCCCCGC
  GGGGCTGGAGCGAGTTCTGTGAGTTGCACGCCGTAGCGGCGGCCCGGGAGCTGGCCCGCCAGTACTGGCT
  GTTCGCCCGGGAGCATCCGCAGCACGCGCCGCTGCGCGCCGAGCTGGTGTCGCTGCAGTTCACCGACCTC
  TTCCAGCGCTACTTCTGCCGCGAGGTGCGCGACGGACGGGCGCCGGGCCGCGACTACCGGGACACAGGCC
  GTGGGCCCCCAGCCAAGGCCGAGGCGTCCCCGGAGCCAGGCCCCGGCCCCGCCGCCCCTGGCCTGCCCAA
  GGCCCGCAGCTCTGAGGAGCTGGCCCCGCCGCGGCCGCCCGGGCCCTGCTCCTTCCAGCACTTTCGCCGC
  AGCCTCCGCCACATCTTCCGCCGCCGCTCGGCCGGGGAGCTGCCAGCGGCCCACACCGCTGCCGCCCCCG
  GGACCCCCGGAGAGGCTGCTGAGACCCCCGCCCGGCCTGGCCTGGCCAAGAAGTTCCTGCCCTGGAGCCT
  GGCCCGGGAGCCGCCACCCGAGGCGCTGAAGGAGGCGGTGCTGCGCTACAGCCTGGCCGACGAGGCCTCC
  ATGGACAGCGGGGCACGCTGGCAGCGCGGGAGGCTGGCGCTGCGCCGGGCCCCGGGCCCCGATGGCCCCG
  ACCGCGTGCTGGAGCTCTTCGACCCACCCAAGAGTTCAAGGCCCAAGCTACAAGCAGCTTGCTCCAGCAT
  CCAGGAGGTCCGGCGGTGCACACGGCTTGAGATGCCTGACAACCTTTACACCTTTGTGCTGAAGGTGAAG
  GACCGGACAGACATCATCTTTGAGGTGGGAGACGAGCAGCAGCTGAATTCATGGATGGCTGAGCTCTCGG
  AGTGCACAGGCCGAGGGCTGGAGAGCACAGAAGCAGAGATGCATATTCCCTCAGCCCTAGAGCCTAGCAC
  GTCCAGCTCCCCAAGGGGCAGCACAGATTCCCTTAACCAAGGTGCTTCTCCTGGGGGGCTGCTGGACCCG
  GCCTGCCAGAAGACGGACCATTTCCTGTCCTGCTACCCCTGGTTCCACGGCCCCATCTCCAGAGTGAAAG
  CAGCTCAGCTGGTTCAGCTGCAGGGCCCTGATGCTCATGGAGTGTTCCTGGTGCGGCAGAGCGAGACGCG
  GCGTGGGGAATACGTGCTCACTTTCAACTTTCAGGGGATAGCCAAGCACCTGCGCCTGTCGCTGACAGAG
  CGGGGCCAGTGCCGTGTGCAGCACCTCCACTTTCCCTCGGTCGTGGACATGCTCCACCACTTCCAGCGCT
  CGCCCATCCCACTCGAGTGCGGCGCCGCCTGTGATGTCCGGCTCTCCAGCTACGTGGTAGTCGTCTCCCA
  ACCACCAGGTTCCTGCAACACGGTCCTCTTCCCTTTCTCCCTTCCTCACTGGGATTCAGAGTCCCTTCCT
  CACTGGGGTTCAGAGTTGGGCCTTCCCCACCTTAGTTCTTCTGGCTGTCCCCGGGGGCTCAGCCCAGAGG
  GTCTCCCAGGGCGATCCTCACCCCCCGAGCAGATCTTCCACCTGGTGCCTTCGCCCGAAGAACTGGCCAA
  CAGCCTGCAGCACCTGGAGCATGAGCCTGTGAATCGAGCCCGGGACTCGGACTACGAAATGGACTCATCC
  TCCCGGAGCCACCTGCGGGCCATAGACAATCAGTACACACCTCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC218359 representing NM_005475
  Red=Cloning site Green=Tags(s)
  
  MNGPALQPSSPSSAPSASPAAAPRGWSEFCELHAVAAARELARQYWLFAREHPQHAPLRAELVSLQFTDL
  FQRYFCREVRDGRAPGRDYRDTGRGPPAKAEASPEPGPGPAAPGLPKARSSEELAPPRPPGPCSFQHFRR
  SLRHIFRRRSAGELPAAHTAAAPGTPGEAAETPARPGLAKKFLPWSLAREPPPEALKEAVLRYSLADEAS
  MDSGARWQRGRLALRRAPGPDGPDRVLELFDPPKSSRPKLQAACSSIQEVRRCTRLEMPDNLYTFVLKVK
  DRTDIIFEVGDEQQLNSWMAELSECTGRGLESTEAEMHIPSALEPSTSSSPRGSTDSLNQGASPGGLLDP
  ACQKTDHFLSCYPWFHGPISRVKAAQLVQLQGPDAHGVFLVRQSETRRGEYVLTFNFQGIAKHLRLSLTE
  RGQCRVQHLHFPSVVDMLHHFQRSPIPLECGAACDVRLSSYVVVVSQPPGSCNTVLFPFSLPHWDSESLP
  HWGSELGLPHLSSSGCPRGLSPEGLPGRSSPPEQIFHLVPSPEELANSLQHLEHEPVNRARDSDYEMDSS
  SRSHLRAIDNQYTPL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005475
• ORF Size: 1725 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005475.3
• RefSeq Size: 5423 bp
• RefSeq ORF: 1728 bp
• Locus ID: 10019
• UniProt ID: Q9UQQ2
• Cytogenetics: 12q24.12
• Domains: SH2, PH
• Protein Pathways: Neurotrophin signaling pathway
• MW: 63 kDa
• Gene Summary: This gene encodes a member of the SH2B adaptor family of proteins, which are involved in a range of signaling activities by growth factor and cytokine receptors. The encoded protein is a key negative regulator of cytokine signaling and plays a critical role in hematopoiesis. Mutations in this gene have been associated with susceptibility to celiac disease type 13 and susceptibility to insulin-dependent diabetes mellitus. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2014]