HNF6 (ONECUT1) (NM_004498) Human Tagged ORF Clone

CAT#: RC218219

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ONECUT1 (Myc-DDK-tagged)-Human one cut homeobox 1 (ONECUT1)



  "NM_004498" in other vectors (6)

Reconstitution Protocol

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 477.00


Availability*
In Stock

Size
    • 10 ug


Product images

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol ONECUT1
Synonyms HNF-6; HNF6; HNF6A
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC218219 representing NM_004498
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAACGCGCAGCTGACCATGGAAGCGATCGGCGAGCTGCACGGGGTGAGCCATGAGCCGGTGCCCGCCC
CTGCCGACCTGCTGGGCGGCAGCCCCCACGCGCGCAGCTCCGTGGCGCACCGCGGCAGCCACCTGCCCCC
CGCGCACCCGCGCTCCATGGGCATGGCGTCCCTGCTGGACGGCGGCAGCGGCGGCGGAGATTACCACCAC
CACCACCGGGCCCCTGAGCACAGCCTGGCCGGCCCCCTGCATCCCACCATGACCATGGCCTGCGAGACTC
CCCCAGGTATGAGCATGCCCACCACCTACACCACCTTGACCCCTCTGCAGCCGCTGCCTCCCATCTCCAC
AGTCTCGGACAAGTTCCCCCACCATCACCACCACCACCATCACCACCACCACCCGCACCACCACCAGCGC
CTGGCGGGCAACGTGAGCGGTAGCTTCACGCTCATGCGGGATGAGCGCGGGCTGGCCTCCATGAATAACC
TCTATACCCCCTACCACAAGGACGTGGCCGGCATGGGCCAGAGCCTCTCGCCCCTCTCCAGCTCCGGTCT
GGGCAGCATCCACAACTCCCAGCAAGGGCTCCCCCACTATGCCCACCCGGGGGCCGCCATGCCCACCGAC
AAGATGCTCACCCCCAACGGCTTCGAAGCCCACCACCCGGCCATGCTCGGCCGCCACGGGGAGCAGCACC
TCACGCCCACCTCGGCCGGCATGGTGCCCATCAACGGCCTTCCTCCGCACCATCCCCACGCCCACCTGAA
CGCCCAGGGCCACGGGCAACTCCTGGGCACAGCCCGGGAGCCCAACCCTTCGGTGACCGGCGCGCAGGTC
AGCAATGGAAGTAATTCAGGTCAGATGGAAGAGATCAATACCAAAGAGGTGGCGCAGCGTATCACCACCG
AGCTCAAGCGCTACAGCATCCCACAGGCCATCTTCGCGCAGAGGGTGCTCTGCCGCTCCCAGGGGACCCT
CTCGGACCTGCTGCGCAACCCCAAACCCTGGAGCAAACTCAAATCCGGCCGGGAGACCTTCCGGAGGATG
TGGAAGTGGCTGCAGGAGCCGGAGTTCCAGCGCATGTCCGCGCTCCGCTTAGCAGCATGCAAAAGGAAAG
AACAAGAACATGGGAAGGATAGAGGCAACACACCCAAAAAGCCCAGGTTGGTCTTCACAGATGTCCAGCG
TCGAACTCTACATGCAATATTCAAGGAAAATAAGCGTCCATCCAAAGAATTGCAAATCACCATTTCCCAG
CAGCTGGGGTTGGAGCTGAGCACTGTCAGCAACTTCTTCATGAACGCAAGAAGGAGGAGTCTGGACAAGT
GGCAGGACGAGGGCAGCTCCAATTCAGGCAACTCATCTTCTTCATCAAGCACTTGTACCAAAGCA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC218219 representing NM_004498
Red=Cloning site Green=Tags(s)

MNAQLTMEAIGELHGVSHEPVPAPADLLGGSPHARSSVAHRGSHLPPAHPRSMGMASLLDGGSGGGDYHH
HHRAPEHSLAGPLHPTMTMACETPPGMSMPTTYTTLTPLQPLPPISTVSDKFPHHHHHHHHHHHPHHHQR
LAGNVSGSFTLMRDERGLASMNNLYTPYHKDVAGMGQSLSPLSSSGLGSIHNSQQGLPHYAHPGAAMPTD
KMLTPNGFEAHHPAMLGRHGEQHLTPTSAGMVPINGLPPHHPHAHLNAQGHGQLLGTAREPNPSVTGAQV
SNGSNSGQMEEINTKEVAQRITTELKRYSIPQAIFAQRVLCRSQGTLSDLLRNPKPWSKLKSGRETFRRM
WKWLQEPEFQRMSALRLAACKRKEQEHGKDRGNTPKKPRLVFTDVQRRTLHAIFKENKRPSKELQITISQ
QLGLELSTVSNFFMNARRRSLDKWQDEGSSNSGNSSSSSSTCTKA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_004498
ORF Size 1395 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_004498.4
RefSeq Size 1925 bp
RefSeq ORF 1398 bp
Locus ID 3175
UniProt ID Q9UBC0
Cytogenetics 15q21.3
Protein Families ES Cell Differentiation/IPS, Transcription Factors
Protein Pathways Maturity onset diabetes of the young
MW 50.8 kDa
Gene Summary This gene encodes a member of the Cut homeobox family of transcription factors. Expression of the encoded protein is enriched in the liver, where it stimulates transcription of liver-expressed genes, and antagonizes glucocorticoid-stimulated gene transcription. This gene may influence a variety of cellular processes including glucose metabolism, cell cycle regulation, and it may also be associated with cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2012]

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