HNF6 (ONECUT1) (NM_004498) Human Tagged ORF Clone

CAT#: RC218219

ONECUT1 (Myc-DDK-tagged)-Human one cut homeobox 1 (ONECUT1)

Product Images

Circular map for RC218219

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY HNF6 (Cat# RC218219, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HNF6(Cat# TA500196). Positive lysates LY401429 (100ug) and LC401429 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY401429) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC218219 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: HNF6
• Synonyms: HNF-6; HNF6; HNF6A
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC218219 representing NM_004498
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACGCGCAGCTGACCATGGAAGCGATCGGCGAGCTGCACGGGGTGAGCCATGAGCCGGTGCCCGCCC
  CTGCCGACCTGCTGGGCGGCAGCCCCCACGCGCGCAGCTCCGTGGCGCACCGCGGCAGCCACCTGCCCCC
  CGCGCACCCGCGCTCCATGGGCATGGCGTCCCTGCTGGACGGCGGCAGCGGCGGCGGAGATTACCACCAC
  CACCACCGGGCCCCTGAGCACAGCCTGGCCGGCCCCCTGCATCCCACCATGACCATGGCCTGCGAGACTC
  CCCCAGGTATGAGCATGCCCACCACCTACACCACCTTGACCCCTCTGCAGCCGCTGCCTCCCATCTCCAC
  AGTCTCGGACAAGTTCCCCCACCATCACCACCACCACCATCACCACCACCACCCGCACCACCACCAGCGC
  CTGGCGGGCAACGTGAGCGGTAGCTTCACGCTCATGCGGGATGAGCGCGGGCTGGCCTCCATGAATAACC
  TCTATACCCCCTACCACAAGGACGTGGCCGGCATGGGCCAGAGCCTCTCGCCCCTCTCCAGCTCCGGTCT
  GGGCAGCATCCACAACTCCCAGCAAGGGCTCCCCCACTATGCCCACCCGGGGGCCGCCATGCCCACCGAC
  AAGATGCTCACCCCCAACGGCTTCGAAGCCCACCACCCGGCCATGCTCGGCCGCCACGGGGAGCAGCACC
  TCACGCCCACCTCGGCCGGCATGGTGCCCATCAACGGCCTTCCTCCGCACCATCCCCACGCCCACCTGAA
  CGCCCAGGGCCACGGGCAACTCCTGGGCACAGCCCGGGAGCCCAACCCTTCGGTGACCGGCGCGCAGGTC
  AGCAATGGAAGTAATTCAGGTCAGATGGAAGAGATCAATACCAAAGAGGTGGCGCAGCGTATCACCACCG
  AGCTCAAGCGCTACAGCATCCCACAGGCCATCTTCGCGCAGAGGGTGCTCTGCCGCTCCCAGGGGACCCT
  CTCGGACCTGCTGCGCAACCCCAAACCCTGGAGCAAACTCAAATCCGGCCGGGAGACCTTCCGGAGGATG
  TGGAAGTGGCTGCAGGAGCCGGAGTTCCAGCGCATGTCCGCGCTCCGCTTAGCAGCATGCAAAAGGAAAG
  AACAAGAACATGGGAAGGATAGAGGCAACACACCCAAAAAGCCCAGGTTGGTCTTCACAGATGTCCAGCG
  TCGAACTCTACATGCAATATTCAAGGAAAATAAGCGTCCATCCAAAGAATTGCAAATCACCATTTCCCAG
  CAGCTGGGGTTGGAGCTGAGCACTGTCAGCAACTTCTTCATGAACGCAAGAAGGAGGAGTCTGGACAAGT
  GGCAGGACGAGGGCAGCTCCAATTCAGGCAACTCATCTTCTTCATCAAGCACTTGTACCAAAGCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC218219 representing NM_004498
  Red=Cloning site Green=Tags(s)
  
  MNAQLTMEAIGELHGVSHEPVPAPADLLGGSPHARSSVAHRGSHLPPAHPRSMGMASLLDGGSGGGDYHH
  HHRAPEHSLAGPLHPTMTMACETPPGMSMPTTYTTLTPLQPLPPISTVSDKFPHHHHHHHHHHHPHHHQR
  LAGNVSGSFTLMRDERGLASMNNLYTPYHKDVAGMGQSLSPLSSSGLGSIHNSQQGLPHYAHPGAAMPTD
  KMLTPNGFEAHHPAMLGRHGEQHLTPTSAGMVPINGLPPHHPHAHLNAQGHGQLLGTAREPNPSVTGAQV
  SNGSNSGQMEEINTKEVAQRITTELKRYSIPQAIFAQRVLCRSQGTLSDLLRNPKPWSKLKSGRETFRRM
  WKWLQEPEFQRMSALRLAACKRKEQEHGKDRGNTPKKPRLVFTDVQRRTLHAIFKENKRPSKELQITISQ
  QLGLELSTVSNFFMNARRRSLDKWQDEGSSNSGNSSSSSSTCTKA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004498
• ORF Size: 1395 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004498.4
• RefSeq Size: 1925 bp
• RefSeq ORF: 1398 bp
• Locus ID: 3175
• UniProt ID: Q9UBC0
• Cytogenetics: 15q21.3
• Protein Families: ES Cell Differentiation/IPS, Transcription Factors
• Protein Pathways: Maturity onset diabetes of the young
• MW: 50.8 kDa
• Gene Summary: This gene encodes a member of the Cut homeobox family of transcription factors. Expression of the encoded protein is enriched in the liver, where it stimulates transcription of liver-expressed genes, and antagonizes glucocorticoid-stimulated gene transcription. This gene may influence a variety of cellular processes including glucose metabolism, cell cycle regulation, and it may also be associated with cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2012]