MLIP (NM_138569) Human Tagged ORF Clone

CAT#: RC218122

MLIP (Myc-DDK-tagged)-Human muscular LMNA-interacting protein (MLIP)

Product Images

Circular map for RC218122

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  Western blot validation of overexpression lysate (Cat# LY408572) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC218122 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified MLIP protein (Cat# TP318122). The protein was produced from HEK293T cells transfected with MLIP cDNA clone (Cat# RC218122) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MLIP
• Synonyms: C6orf142; CIP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC218122 representing NM_138569
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAACTTGAAAAGCGTGAAAAAAGAAGCTTATTAAACAAGAATTTAGAGGAGAAACTGACGGTCTCTG
  CTGGTGGTTCTGAAGCCAAACCTCTGATCTTCACATTTGTCCCCACTGTCAGAAGACTACCAACCCATAC
  TCAGTTGGCTGACACCTCTAAATTCCTTGTTAAAATTCCAGAAGAATCAAGTGATAAGAGTCCAGAAACT
  GTAAATAGGTCTAAATCCAATGACTACTTGACCTTGAATGCTGGGAGCCAACAAGAGAGAGACCAAGCGA
  AATTGACTTGTCCTTCAGAGGTCAGTGGAACGATTTTACAAGAAAGGGAATTCGAAGCAAACAAACTTCA
  AGGGATGCAGCAAAGTGACCTCTTCAAAGCTGAATATGTCCTTATTGTGGACTCCGAAGGGGAAGATGAG
  GCTGCAAGCAGAAAAGTTGAACAAGGCCCCCCAGGGGGGATTGGCACCGCAGCTGTCCGGCCCAAGTCTC
  TAGCTATCTCGTCCAGTCTGGTCTCTGATGTAGTGCGTCCCAAAACACAGGGGACTGATCTCAAGACCTC
  ATCACATCCTGAAATGCTTCATGGGATGGCCCCTCAGCAAAAGCATGGGCAGCAATACAAGACCAAGTCA
  AGCTACAAGGCTTTTGCAGCAATCCCTACAAACACATTGCTTTTGGAACAGAAGGCACTAGATGAACCAG
  CCAAGACTGAAAGTGTCTCCAAGGACAACACATTAGAACCACCAGTGGAGCTCTATTTTCCTGCACAGCT
  CAGGCAGCAAACTGAAGAGCTCTGTGCTACCATTGATAAGGTCTTACAGGATTCCTTGTCTATGCATTCT
  TCTGATTCTCCTTCAAGGTCCCCAAAGACATTGTTGGGTTCTGACACAGTCAAAACTCCTACAACTCTTC
  CAAGAGCAGCTGGTCGAGAAACCAAATATGCAAATCTCTCCTCACCATCTTCTACAGTATCTGAGAGTCA
  GCTGACTAAGCCTGGAGTAATTCGCCCAGTACCTGTAAAATCCAGAATATTACTGAAAAAAGAGGAGGAA
  GTCTATGAACCCAACCCTTTCAGTAAATACTTGGAAGATAACAGCGACCTCTTTTCTGAACAGGATGTAA
  CAGTCCCTCCCAAGCCTGTCTCGCTCCATCCTTTATATCAGACTAAACTCTATCCTCCTGCTAAGTCACT
  GCTGCATCCACAGACCCTCTCACATGCTGACTGTCTTGCCCCAGGACCCTTCAGTCATCTGTCCTTCTCC
  TTGAGTGATGAACAGGAGAATTCTCACACCCTCCTCAGTCACAACGCATGCAACAAGCTGAGTCATCCAA
  TGGTGGCTATTCCTGAACATGAAGCTCTTGATTCCAAAGAGCAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC218122 representing NM_138569
  Red=Cloning site Green=Tags(s)
  
  MELEKREKRSLLNKNLEEKLTVSAGGSEAKPLIFTFVPTVRRLPTHTQLADTSKFLVKIPEESSDKSPET
  VNRSKSNDYLTLNAGSQQERDQAKLTCPSEVSGTILQEREFEANKLQGMQQSDLFKAEYVLIVDSEGEDE
  AASRKVEQGPPGGIGTAAVRPKSLAISSSLVSDVVRPKTQGTDLKTSSHPEMLHGMAPQQKHGQQYKTKS
  SYKAFAAIPTNTLLLEQKALDEPAKTESVSKDNTLEPPVELYFPAQLRQQTEELCATIDKVLQDSLSMHS
  SDSPSRSPKTLLGSDTVKTPTTLPRAAGRETKYANLSSPSSTVSESQLTKPGVIRPVPVKSRILLKKEEE
  VYEPNPFSKYLEDNSDLFSEQDVTVPPKPVSLHPLYQTKLYPPAKSLLHPQTLSHADCLAPGPFSHLSFS
  LSDEQENSHTLLSHNACNKLSHPMVAIPEHEALDSKEQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_138569
• ORF Size: 1374 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_138569.2, NP_612636.2
• RefSeq Size: 1740 bp
• RefSeq ORF: 1377 bp
• Locus ID: 90523
• UniProt ID: Q5VWP3
• Cytogenetics: 6p12.1
• MW: 50.2 kDa
• Gene Summary: Required for precocious cardiac adaptation to stress through integrated regulation of the AKT/mTOR pathways and FOXO1. Regulates cardiac homeostasis and plays an important role in protection against cardiac hypertrophy. Acts as a transcriptional cofactor, represses transactivator activity of ISL1 and MYOCD.[UniProtKB/Swiss-Prot Function]